Hollow polymer microspheres coated with nano-hydroxyapatite and its preparation method and application
A nano-hydroxyapatite and polymer technology, which is applied to the preparation of microspheres, microcapsule preparations, medical preparations of non-active ingredients, etc., can solve the problem of poor biocompatibility and the lack of nano-hydroxyapatite hollow polymers. Microspheres, confinement and other problems, to achieve the effect of improving hydrophilicity, facilitating adhesion and growth, and making the preparation process simple and easy
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Embodiment 1
[0072] Preparation of Example 1 HAp-coated HPC-g-PCL microspheres
[0073] (1) Synthesis of hydroxypropyl cellulose grafted caprolactone copolymer (HPC-g-PCL)
[0074] After fully drying ε-CL (ε-caprolactone, Aladdin Reagent Company) and hydroxypropylcellulose (HPC) (vacuum drying at 37°C), weigh 1 g of liquid ε-CL, and add 0.1 g of HPC Add it into ε-CL, after the HPC is completely dissolved in ε-CL, raise the reaction temperature to 105°C, add 50 μL of stannous octoate as a catalyst, and react for 24 hours under the protection of nitrogen, and dissolve the obtained product in 10-20 mL of tetrahydrofuran ( THF), add n-hexane equal to the volume of THF to precipitate the generated HPC-g-PCL, and dry the collected precipitate after repeating three times. According to the weighing method, the product grafting rate is calculated, and the grafting rate GY=790%, wherein the calculation formula is as shown in formula 1-1:
[0075] GY=(W 2 -W 1 )×100 / W 1 (1-1)
[0076] Where: W...
Embodiment 2
[0083] The preparation of embodiment 2 indomethacin (Indomethacin) drug-loaded microspheres
[0084] (1) Synthesis of hydroxypropyl cellulose grafted caprolactone copolymer (HPC-g-PCL)
[0085] With embodiment 1 step (1).
[0086] (2) Preparation of HAp-coated HPC-g-PCL drug-loaded microspheres
[0087] ① Disperse 0.1g of nano-hydroxyapatite (Hydroxylapatite, HAp) in 100mL of water to prepare a 0.1wt% HAp dispersion as the water phase;
[0088] ②Dissolve 0.3g of HPC-g-PCL and 0.1g of oil-soluble drug indomethacin (Indomethacin) in 10mL of dichloromethane to obtain 3wt% (calculated by HPC-g-PCL) of HPC-g-PCL and indomethacin Domethacin mixed solution in dichloromethane, as the oil phase;
[0089] ③Take 1mL of the oil phase solution, add 5mL of the water phase dispersion (oil-water ratio 1:5), oscillate to obtain an emulsion, leave it to evaporate at room temperature (stand for 1-3 days), and dry it in vacuum at 37°C to obtain indomethacin drug microspheres. The cavity of t...
Embodiment 3
[0091] According to the method of Example 2, the difference is: the oil-water ratio in step (2) ③ is replaced by 1:3 (take 1mL oil phase solution, add 3mL water phase dispersion liquid), 1:4 (get 1mL oil phase solution, Add 4mL of the aqueous phase dispersion), 1:6 (take 1mL of the oil phase solution, add 6mL of the aqueous phase dispersion), and prepare indomethacin drug-loaded microspheres respectively.
[0092] The indomethacin drug-loaded microspheres prepared under different oil-water ratios were observed under a scanning electron microscope (SEM) and their particle sizes were counted. The results are as follows: Figure 4 Shown: 4a is the microspheres prepared when the oil-water ratio is 1:3, with an average diameter of 66.8 μm; 4b is the microspheres prepared with an oil-water ratio of 1:4, with an average diameter of 60.7 μm; The prepared microspheres have an average diameter of 35.9 μm; 4d is the microspheres prepared when the oil-water ratio is 1:6, with an average d...
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