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Paenarthrobacter nicotinovorans and application thereof in preventing and treating diseases

A technology of Bacteroides nicotinica and Bacteroides nicotine, applied in the direction of application, bacteria, and microbial-based methods, can solve problems such as food safety, antibiotic microbial resistance, environmental pollution, etc., and achieve the effect of reducing the problem of pesticide abuse

Active Publication Date: 2020-01-21
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the extensive use of chemical pesticides has brought about a series of serious problems such as environmental pollution, ecological balance disruption, pathogenic bacteria resistance and food safety, etc. In addition, the abuse of antibiotics has also caused the emergence of microbial resistance

Method used

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  • Paenarthrobacter nicotinovorans and application thereof in preventing and treating diseases
  • Paenarthrobacter nicotinovorans and application thereof in preventing and treating diseases
  • Paenarthrobacter nicotinovorans and application thereof in preventing and treating diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 The acquisition and identification of Bacteroides nicotinica strain D-9

[0041] 1. Isolation and screening of strain D-9

[0042] (1) Soil sample collection: Soil samples collected from the Arboretum of South China Agricultural University and the orchid greenhouse as microbial sources

[0043] Soil samples were collected from South China Agricultural University in Guangdong Province, bagged, stored, and taken back to the laboratory as a microbial source for strain isolation.

[0044] (2) Enrichment culture of bacterial strains: prepare basal salt (MSM) medium, and divide into 100 mL Erlenmeyer flasks, 50 mL for each bottle. Weigh 5g of soil sample, add soil sample (5g) and AHLs signal molecule (C6O3HSC, 100mmol, 12.5μL) into Erlenmeyer flask under sterile conditions, and place in 200rpm, 30℃ shaker for 7 days. After about a week, take out 1mL of the solution under sterile conditions, increase the concentration of AHLs signal molecules to twice the original ...

Embodiment 2

[0058] Antibiotic susceptibility analysis of embodiment 2 bacterial strain D-9

[0059] 1. In order to better study the biocontrol potential of the bacterial strain D-9 obtained in Example 1, we conducted in-depth research on the biological characteristics of the bacterial strain. The sensitivity of strain D-9 to different antibiotics was experimentally studied as follows:

[0060] Streak and activate the strain D-9 on a solid LB plate, pick a single colony and inoculate it into 1mL liquid LB, and culture it at 30°C and 200rpm until the bacterial liquid OD 600 The value is between 1.0 and 1.5; 13 concentration gradients are set for each antibiotic for detection, which are 5 μg / mL, 10 μg / mL, 20 μg / mL, 30 μg / mL, 40 μg / mL, 50 μg / mL, 100 μg / mL, 150 μg / mL , 200μg / mL, 250μg / mL, 300μg / mL, 350μg / mL, 400μg / mL, and a blank control. Six antibiotics including kanamycin (kan), ampicillin (amp), gentamicin (gen), streptomycin (str), carbenicillin (carb) and tetracycline (TC) were added to...

Embodiment 3

[0062] Example 3 Identification of Quenching Activity of Bacterial Strain D-9

[0063] 1. Strain cultivation and sample collection: Under the condition of 30°C, activate the strain D-2 on the LB solid plate. Pick a single colony and inoculate it into LB liquid medium, and culture overnight at 30°C and 200rpm to obtain a bacterial solution. Take a certain volume (V=1 / OD 600 ) was centrifuged at 4000rpm for 10min, the supernatant was discarded to obtain an OD 600 value bacteria. Will 1 OD 600 Value of the bacteria, inoculated into 1mL MSM inorganic salt medium with different AHLs as the sole carbon source. The reaction mixtures were placed in 2 mL centrifuge tubes, and the centrifuge tubes were incubated at 30° C. and 200 rpm for 24 h. After 24 hours, take 5 μL of the reaction mixture and spot it on the top of a 1 cm wide MM agar strip, and then spot the reporter strain (Agrobacterium tumefaciens NT1) bacteria solution below. Then place the agar strips with the reaction mi...

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Abstract

The present invention discloses paenarthrobacter nicotinovorans and an application thereof in preventing and treating diseases. The microorganism group induction signal quenching bacterium paenarthrobacter nicotinovorans strain D-9 is screened and preserved at Guangdong Culture Collection Center on June 14, 2019, and has a preservation number of GDMCC No.60691. The strain has activity of quenchingquorum sensing signal molecules of N-acyl homoserine lactones (AHLs), besides has stable and significant quenching effect, and thus has huge application potential in preventing and treating harms ofpectobacterium carotovorum subsp. carotovora (Pcc) and other pathogenic bacteria mediated by depending AHLs quorum sensing signals. The anti-pathogenic drug with quorum quenching as a mode of action has broad application prospects.

Description

technical field [0001] The invention belongs to the technical field of biological control. More specifically, it relates to a Bacteroides nicotinica and its application in disease control. Background technique [0002] There are many kinds of cruciferous vegetables, mainly including Chinese cabbage, small green vegetables, cabbage, radish, cauliflower, etc., which occupy a large share in my country's vegetable sales market. Soft rot, viral disease and downy mildew are the three major diseases of cruciferous vegetables worldwide (Yan Bangbin, Fan Ping. Comprehensive control of soft rot in cruciferous vegetables[J]. Sichuan Agricultural Science and Technology, 2001,11: 32). Among them, soft rot not only harms cruciferous vegetables, but also can infect crops such as lettuce, potato, tomato, eggplant, pepper, cucumber, carrot, celery, green onion, kiwi fruit, etc., but cabbage, radish and cabbage are the most serious victims, causing serious economic loss. In severe years o...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/20A01P1/00C12R1/01
CPCA01N63/00C12N1/20C12N1/205C12R2001/01
Inventor 陈少华李绮婷梁梓侨范兴辉李欣林媛张炼辉
Owner SOUTH CHINA AGRI UNIV
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