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Fusarium redolens fungus HNU066 and application thereof in degradation of hydrogen sulfide containing exhaust gas

A technology of Fusarium and fungus, which is applied in the field of environmental pollution control, can solve the problems of difficult preservation, short effective time, and high maintenance cost, and achieve the effects of shortening domestication time, good preservation, and improved removal efficiency

Active Publication Date: 2020-02-21
HANGZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current bio-trickling filter reactors have disadvantages such as low efficiency, short effective time, difficult storage and high maintenance costs, which limit the application of bio-trickling filter reactors in the field of exhaust gas purification to a certain extent.

Method used

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  • Fusarium redolens fungus HNU066 and application thereof in degradation of hydrogen sulfide containing exhaust gas
  • Fusarium redolens fungus HNU066 and application thereof in degradation of hydrogen sulfide containing exhaust gas
  • Fusarium redolens fungus HNU066 and application thereof in degradation of hydrogen sulfide containing exhaust gas

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Screening, isolation, and cultivation of the aromatic Fusarium fungus "HNU066"

[0031] The aromatic Fusarium fungus "HNU066" was isolated from the bioreactor of Rousselot (Wenzhou) Gelatin Co., Ltd. ( figure 1 ). The separation method is as follows: Take 5 g of domesticated biotrickling filter reactor sludge, dilute with distilled water to three different concentration gradients of 10 times, 100 times and 1000 times, and spread them on the PDA fungal solid medium respectively. Each petri dish was coated with one milliliter of diluted sludge leaching solution, and cultured at a constant temperature of 25 degrees in the dark. After cultivating for one week, the single colonies formed were observed. Pick the mycelium from the edge of the selected single colony, transfer to a fresh PDA plate for streak separation and culture, repeat the streak operation until a pure culture is obtained, and transfer to a PDA slant for storage.

Embodiment 2

[0032] Example 2: Morphological and molecular biological identification of the aromatic Fusarium fungus "HNU066"

[0033] Under a microscope, observe the characteristics of endophytic fungi such as colonies, hyphae, and spores, and identify them by referring to the "Handbook of Fungal Identification". Specifically, the screened aromatic Fusarium fungus "HNU066" strain was planted on a PDA plate by spot seeding method, placed at a constant temperature of 25 degrees for cultivation, and the morphological characteristics of the strain were observed with naked eyes, including colony shape, color, size, Edge characteristics, hyphae properties, growth speed and other characteristics. The hyphae on the surface of the colony were picked and placed on an optical microscope (Japan, OLYMPUS) to observe the presence or absence of spores and sporulation structures.

[0034] The morphological characteristics of the aromatic Fusarium fungus "HNU066" of the present invention are as follows:

...

Embodiment 3

[0036] Example 3: Molecular biological identification of the fungus Fusarium aromaticum "HNU066"

[0037] Genomic DNA of the fungus Fusarium aromaticum "HNU066" was extracted by a general fungal DNA extraction method. The extraction method is as follows: take 1.5mL bacterial liquid, 12000×g, 2min, collect the bacterial cells; add 500μL 5×CTAB (containing 1% β-mercaptoethanol), quick-freeze in liquid nitrogen for 30s, then transfer to 65°C for 30s, Repeat the above process 3 times; vortex at high speed for 3-5min, incubate at 65°C for 20min, add 2ml of phenol:chloroform:isoamyl alcohol (25:24:1, V / V / V), 12000×g, 10min, take supernatant; add 2 times the volume of absolute ethanol to the supernatant, and centrifuge again after resting for 20 minutes; pour off the supernatant, wash the precipitate with 70% alcohol; dry under natural conditions to obtain fungal DNA for later use.

[0038] Using fungal universal primers ITS5 (5'-GGAAGTAAAAGTCGTAACAAGG-3') and ITS4 (5'-TCCTCCGCTTATT...

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Abstract

The invention discloses a Fusarium redolens fungus HNU066 and an application thereof in degradation of hydrogen sulfide containing exhaust gas. The Fusarium redolens fungus HNU066 disclosed by the invention has a collection number of CCTCC No: M 2019500. The fungus HNU066 is applied to the degradation of the hydrogen sulfide containing exhaust gas. The richness of the fungus HNU066 in active fillers of a biological trickling filtration column reactor is improved, and the efficiency of clearing of the hydrogen sulfide containing exhaust gas by bioactive fillers can be increased. After the reactor added with the Fusarium redolens fungus HNU066 is in a stable stage, the hydrogen sulfide containing exhaust gas absorption clearing efficiency is increased by 34.5% compared with that of a controlgroup.

Description

technical field [0001] The invention belongs to the field of environmental pollution control, and relates to a sulfur fungus HNU066 of the genus Fusarium redolens and its application in biodegradation of industrial waste gas containing hydrogen sulfide. Background technique [0002] With the intensification of environmental pollution, industrial waste gas emissions have become a major culprit affecting people's healthy life. In the actual industrial waste gas emissions, the waste gas containing hydrogen sulfide occupies an important proportion, so it is listed as one of the gas pollutants that are prioritized by the country. Hydrogen sulfide is a malodorous gas with the smell of rotten eggs, which seriously affects the lives of surrounding residents. Therefore, the discharge of a large amount of hydrogen sulfide-containing waste gas seriously damages human health. In addition, hydrogen sulfide emitted into the atmosphere is oxidized to form sulfate ions, which is the main c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14B01D53/84B01D53/52C12R1/77
CPCB01D53/52B01D53/84B01D2251/95C12N1/14C12N1/145C12R2001/77Y02A50/20
Inventor 刘奇沈晨佳倪建国成卓韦宋欣欣杨华云李伟东
Owner HANGZHOU NORMAL UNIVERSITY
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