Tetracycline antibiotic degrading bacterium and application thereof in remediation of soil pollution

A tetracycline and soil pollution technology, which is applied in the field of biological treatment of environmental pollutants, can solve problems such as tetracycline antibiotic pollution, and achieve the effects of improving bioavailability, safe and effective pollution, and good environmental adaptability.

Inactive Publication Date: 2020-03-24
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a tetracycline antibiotic degrading bacterium in order to solve the pollution problem of tetracycline antibiotics in the soil environment

Method used

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  • Tetracycline antibiotic degrading bacterium and application thereof in remediation of soil pollution
  • Tetracycline antibiotic degrading bacterium and application thereof in remediation of soil pollution
  • Tetracycline antibiotic degrading bacterium and application thereof in remediation of soil pollution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0081] Example 1: The tolerance of tetracycline antibiotics degrading bacteria biological agent to oxytetracycline in soil and the remediation test of oxytetracycline contaminated soil

[0082] After activating Pseudomonas PN1, inoculate it in a solid medium, and culture it in a constant temperature and humidity incubator at 28°C for 48 hours until the biomass of the microorganism reaches the maximum, and then wash down the bacteria with sterile normal saline Make a bacterial suspension with an OD600 of 0.5±0.01 as the PN1 inoculum.

[0083]The red soil for testing was taken from the Arboretum of South China Agricultural University. Its texture is silty sandy soil, pH value (water / soil=2.5 / 1) is 4.52, organic matter is 0.57g / kg, moisture content is 0.85%, nitrogen, phosphorus Potassium is 0.71g / kg, 0.72g / kg, 2.61g / kg, oxytetracycline is not detected, pass through a 2mm sieve; add oxytetracycline solution dissolved in methanol to 100g of soil passed through a 2mm sieve, and sti...

Embodiment 2

[0091] Embodiment 2: the tolerance of tetracycline antibiotics degrading bacteria biological agent to other tetracycline antibiotics (such as tetracycline) in soil and the remediation test of other tetracycline antibiotic contaminated soil

[0092] After activating Pseudomonas PN1, inoculate it in a solid medium, and culture it in a constant temperature and humidity incubator at 28°C for 48 hours until the biomass of the microorganism reaches the maximum, and then wash the bacteria with sterile normal saline Then make a bacterial suspension with an OD600 of 0.5±0.01 as the inoculum.

[0093] The red soil for testing was taken from the Arboretum of South China Agricultural University. Its texture is silty sandy soil, pH value (water / soil=2.5 / 1) is 4.52, organic matter is 0.57g / kg, moisture content is 0.85%, nitrogen, phosphorus , Potassium is 0.71g / kg, 0.72g / kg, 2.61g / kg, tetracycline is not detected, pass through a 2mm sieve, add tetracycline solution dissolved in acetone to 1...

Embodiment 3

[0102] Embodiment 3: the impact test of non-sterilized and sterilized soil

[0103] The preparation of inoculum is the same as in Example 1, and the added bacteria of each treatment is an immobilized microbial inoculum, and the preparation of test soil and bagasse immobilized microbial inoculum is the same as in Example 1 and Example 2, and the soil concentration in the contaminated soil is measured before the experiment. The initial concentrations of mycin and tetracycline were 100.28 mg / kg and 83.13 mg / kg.

[0104] This embodiment sets the following 4 processes:

[0105] Treatment 1: Non-sterilized soil control treatment (without adding bacteria)

[0106] Treatment 2: Sterilized soil control treatment (without adding bacteria)

[0107] Treatment 3: Treatment with non-sterilized soil plus immobilized microbial agent

[0108] Treatment 4: treatment with sterilized soil plus immobilized microbial agent.

[0109] There were 21 beakers under each treatment, samples were taken...

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Abstract

The invention discloses a tetracycline antibiotic degrading bacterium and its application in remediation of soil pollution. The degrading bacterium is Pseudomonas nitroreducens. Each single strain, which is obtained after pig manure samples undergo screening, domestication, isolation and purification with tetracycline antibiotic tolerant bacteria, is inoculated into an inorganic salt medium containing the only carbon source of tetracycline antibiotics for degradation experiments and screening to obtain the strain with the strongest ability of degrading tetracycline antibiotics in the inorganicsalt medium. According to the invention, immobilized microbial inoculum is safe, effective, harmless, environmentally-friendly, green and cheap in repairing soil tetracycline antibiotic oxytetracycline and tetracycline pollution without causing secondary pollution; meanwhile, the bioavailability of tetracycline antibiotics can be improved, and the efficiency of microbial degradation of tetracycline antibiotics is further enhanced.

Description

【Technical field】 [0001] The invention relates to a tetracycline antibiotic-degrading bacterium and its application in soil pollution restoration, and belongs to the technical field of biological treatment of environmental pollutants. 【Background technique】 [0002] Tetracycline antibiotics are cheap and have good antibacterial effect. They are also a growth promoter and are often widely used in animal husbandry. They are one of the most widely used antibiotic drug additives in my country. Since it cannot be completely absorbed or metabolized by animals, about 30%-90% is discharged into the environment in the form of the original drug or metabolites through animal feces. Manure containing tetracycline antibiotics and their derivatives is directly fertilized on the soil without standardized treatment, resulting in a large amount of tetracycline antibiotics entering livestock products and crops, posing a hazard to human health and public health. Long-term discharge of low dos...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C12N1/36C12N11/02B09C1/10C02F3/34C12R1/38C02F101/38
CPCB09C1/10C02F3/34C02F2101/38C12N1/02C12N1/20C12N1/36C12N11/02C12N1/205C12R2001/38
Inventor 赵月春洪夏晓
Owner SOUTH CHINA AGRI UNIV
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