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Method for reducing and adsorbing heavy metal chromium by immobilized microorganisms

A technology of microbial agent and immobilized pellets, applied in the field of environmental biology, can solve the problems of high operating cost, low efficiency, secondary pollution of water and soil, etc., and achieve the effect of prolonging the use time and frequency

Active Publication Date: 2020-04-03
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods generally have problems such as high energy consumption, high operating cost, complicated operation, and low efficiency when dealing with low-concentration heavy metals.
Physical and chemical remediation methods can also cause secondary pollution of water and soil

Method used

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  • Method for reducing and adsorbing heavy metal chromium by immobilized microorganisms
  • Method for reducing and adsorbing heavy metal chromium by immobilized microorganisms
  • Method for reducing and adsorbing heavy metal chromium by immobilized microorganisms

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Example 1: Preparation of mixed microbial agent for reducing hexavalent chromium and chromium reduction experiment

[0035] (1) Preparation of mixed bacterial agent:

[0036] (1) Select two kinds of chromium-reducing bacteria, Bacillus cereus DIF1 and Rhodococcus rubrum DIF2, and inoculate them in LB liquid medium respectively; cultivate them for 8-12 hours at 28-30 degrees Celsius and 120 rpm, and the value of OD600 is 0.8 -1.0;

[0037] The LB medium formula is tryptone 10g / L, yeast extract 5g / L, sodium chloride 10g / L, pH7.0;

[0038] (2) choose each 10ml of two kinds of bacterial solutions to inoculate in 100ml of sterilized fresh medium, continue to cultivate for 8-12 hours under the conditions of step (1), the OD600 value reaches 1.0-1.2, and the bacterial solutions are for standby;

[0039] (2) Hexavalent chromium reduction experiment:

[0040] Verification experiment of Cr(VI) reducing ability of mixed bacterial agents: prepare LB solution, add 2mmol / L anhydro...

Embodiment 2

[0041] Embodiment 2: Preparation of gel microspheres and chromium reduction effect

[0042] (1) preparation of activated carbon loaded iron oxide, comprising the following steps:

[0043] (1) 40gFe(NO3) 3 9H 2 Dissolve O in 500mL of deionized water, add 2-4g of activated carbon, and stir;

[0044] (2) Add 500 mL of 1mol / L sodium hydroxide solution dropwise, stir, and when the pH of the solution is adjusted to between 7-8, stop adding sodium hydroxide dropwise;

[0045] (3) The precipitated product was centrifuged at 4000 g for 20 min, rinsed with pure water, centrifuged again, sterilized, and dried at 100 degrees Celsius for later use.

[0046] (2) Preparation of gel microspheres, comprising the following steps:

[0047] (1) Immerse the prepared activated carbon loaded with iron oxide in the bacterial agent suspension culture solution in a mass ratio of 1.8-2.5% for 8-12 hours;

[0048](2) adding alginic acid of 2-3% mass concentration and polyvinyl alcohol of 2-3% mass c...

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Abstract

The invention belongs to the technical field of environmental biology, and particularly relates to a method for reducing and adsorbing heavy metal chromium by immobilized microorganisms. According tothe method, a mixed microbial agent of bacillus cereus and rhodococcus ruber is immobilized by gel microspheres, and heavy metal chromium pollutants are reduced; and the mixed microbial agent can reduce ferric oxide into ferrous ions by using ferric oxide as an electron donor, so that reduction of hexavalent chromium is promoted. The invention further provides a method for immobilizing the mixed microbial agent. The method comprises the following steps: using activated carbon loaded ferric oxide as a carrier, adsorbing the mixed microbial agent and promoting the mixed microbial agent to grow by using the ferric oxide; and, embedding the carrier adsorbing the mixed microbial agent by using sodium alginate and polyvinyl alcohol as embedding agents, so as to prepare microbial agent immobilized microspheres. The microbial agent immobilized microspheres can provide a stable growth environment for the mixed microbial agent, adapt to a toxic environment, and efficiently reduce and adsorb heavy metal ions such as chromium.

Description

technical field [0001] The invention relates to a method for immobilizing microorganisms to reduce and adsorb heavy metal chromium, which belongs to the field of environmental biotechnology. Background technique [0002] Dissimilatory iron-reducing bacteria refer to a group of microorganisms that can use Fe(III) as the only electron acceptor, reduce Fe(III), simultaneously oxidize organic carbon sources, and obtain energy from them for their own growth. Dissimilatory iron-reducing bacteria can reduce a variety of metal ions and remove organic matter and heavy metals during bacterial respiration and growth. Iron reduction is an important microbial metabolic pathway and an important link in the geochemical cycle. The Fe(II) produced during the reduction of dissimilated iron can catalyze the reduction of Cr(VI), which provides a possibility for the treatment of Cr(VI) pollution. [0003] Chromium is mainly used in metal processing, electroplating, tanning and other industries...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N11/14C12N11/10C12N11/084C12N11/04C02F3/34C12R1/085C12R1/01C02F101/22
CPCC12N1/20C12N11/14C12N11/10C12N11/08C12N11/04C02F3/34C02F2101/22Y02W10/10
Inventor 迟慧梅
Owner SOUTHEAST UNIV
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