Anti-infection tissue engineering skin scaffold and preparation method thereof

Abstract

The invention discloses an anti-infection tissue engineering skin scaffold which is of an upper and lower double-layer structure, said layers are combined together through glutaraldehyde cross-linkingaction, and the scaffold has good mechanical strength. Nano-silver inside fibers in the upper epidermal scaffold polycaprolactone-polyethylene glycol-polycaprolactone/nano-silver/chitosan nano-yarn layer and chitosan on the surfaces of the fibers synergistically exert an antibacterial effect, and zinc acetate is uniformly distributed in fibroin/hyaluronic acid fibers of the lower dermal scaffold,so that bacterial aggregation can be inhibited, and an anti-infection effect is exerted; the lower layer is a nanofiber bundle woven scaffold, and the structure, the pore size and the porosity of thefiber bundle scaffold can be freely adjusted through weaving; the scaffold with orderly and regular arrangement of fiber bundles and the pore size average value close to the cell size can be prepared, cells can enter the scaffold, and directional growth of tissue is guided.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to an anti-infection tissue engineering skin scaffold and a preparation method thereof. Background technique [0002] As the largest organ of the human body, the skin plays a role in protecting the human body from the invasion of external objects. Damage to the skin can lead to the invasion of bacteria and pathogens, and severe cases can lead to death. Therefore, in the past three decades, a large number of studies on skin scaffolds have been carried out in the fields of biomedical materials and tissue engineering. Human skin is a double-layered structure consisting of epidermis and dermis. The epidermis is mainly composed of dense keratinocytes, which play a protective role; the dermis is mainly composed of fibroblasts and the collagen secreted by them, which has good elasticity and mechanical strength, and plays an important role in the skin regeneration process. ...

AI Technical Summary

Problems solved by technology

However, the nanofibrous scaffold prepared by electrospinning has disordered fiber arrangement and cannot play a guiding role in cell adhesion and migration.

Most of the electrospun fiber membranes are easy to shrink and deform, and the fiber stability is insufficient. When used as an epidermal scaffold for skin tissue engineering, it cannot meet the requirements of structural stability; the dermal scaffold structure has a small nanofiber pore size and a dense structure, which is not conducive to cell regeneration. Access to the inside of the scaffold to form a three-dimensional culture

When preparing scaffolds by 3D printing, although the porosity and pore size can be adjusted, which solves the problem of compact structure of electrospun fibers, since the printing materials need to reach a certain hardness to obtain the designed shape and structure, this results in the application of 3D printing scaffolds. When the skin tissue engineering is too hard, it is not easy to adhere to the wound surface, which is not conducive to cell adhesion and migration

[0004] Skin and soft tissue damage can easily lead to infection, aggravate wound ulceration, and affect tissue repair. Therefore, at present, antibiotics such as gentamicin and cephalosporins are often loaded into tissue engineering skin scaffold materials to improve their anti-infection ability. However, if the dosage of antibiotics is too large and the release concentration is high, it will cause tissue damage

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