Hemostatic microspheres for arteriovenous and visceral hemorrhage and preparation method thereof

Abstract

The invention discloses hemostatic microspheres for arteriovenous and visceral hemorrhage and a preparation method thereof. The preparation method includes the following steps that 1, soluble silk fibroin is dissolved in an inorganic salt solution, and a silk fibroin solution with the mass-volume percentage concentration of 1%-7% is obtained for standby application; dissolving sodium alginate intoanother inorganic salt solution to obtain a sodium alginate solution with the mass-volume percent concentration of 0.5%-5% for later use; (2) mixing the silk fibroin solution obtained in the step (1)with a sodium alginate solution to obtain a water phase; adding span 80 and Tween 80 into liquid paraffin, and then performing water bath heating and uniform stirring to obtain an oil phase; (3) adding the water phase obtained in the step (2) into the oil phase, uniformly stirring the materials, adding a cross-linking agent, and reacting for a period of time to obtain a microsphere solution; and(4) eluting the microsphere solution obtained in the step (3) with a solvent for multiple times, filtering and drying to obtain the hemostatic microsphere. The hemostatic microsphere is fast in hemostatic effect and excellent in biocompatibility, and can be degraded in vivo.

Description

technical field [0001] The invention belongs to the field of biological materials, and in particular relates to a hemostatic microsphere for arteriovenous and visceral bleeding and a preparation method thereof. Background technique [0002] Trauma bleeding is unavoidable in daily life. Minor trauma is not fatal enough. If the blood loss exceeds 30% of the whole body in a short period of time, it will be life-threatening. (Macleod, J. B., Lynn, M., Mckenney, M. G., Cohn, S. M., & Murtha, M. (2003). Early coagulopathy predicts mortality intrauma. The Journal of Trauma: Injury, Infection, and Critical Care, 55(1) ,39-44.) Excessive blood loss is an important cause of death in wars, natural disasters, traffic accidents and other emergencies. According to statistics, on average, one person is injured every 3 seconds in the world, and one person dies in a traffic accident every 70 seconds. In major traffic accidents, the main cause of death is the rupture of viscera caused by th...

AI Technical Summary

Problems solved by technology

Although there are many kinds of existing hemostatic materials, they all have some shortcomings in different degrees. For example, gauze, bandages and other products are cheap and easy to obtain, but their hemostatic effect is poor, they are easy to adhere to the wound, and they are easy to cause secondary damage to the body when changing dressings. The hemostatic effect of zeolite type is better, and the Quick-Clot porous zeolite hemostatic powder launched by Z-MEDICA Company of the United States in 2002, because of its High specific surface area, good adsorption, good hemostatic effect on both arteries and veins, but its biggest disadvantage is that it will release a lot of heat after acting on the wound, the highest temperature can reach more than 100 ℃, causing damage to the body tissue Severe thermal injury; fibrin products are a biological product extracted from bovine plasma, which is more suitable for some irregular wounds that are inconvenient to dress, but its source is limited, and fibrin products may cause wound suppuration and infection , has potential immunogenicity, and the wound healing time is longer, it needs to be used at 37°C, the production cost is higher, and the effect on arterial hemostasis is poor

Other common polymer hemostatic materials such as chitosan, which is the only positively charged alkaline polysaccharide among natural products, has a certain antibacterial effect, can inhibit the growth of fibroblasts, and reduce the formation of scar tissue. Formation, the hemostatic effect is good for some mild wounds on the surface, but the hemostatic effect is not obvious for some severe traumatic bleeding; there are also some polymers such as polyvinyl alcohol, which have a better hemostatic effect, but they cannot be degraded in the body and require two It is taken out in one operation, which causes a certain burden on the patient's body and economy.

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