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A method for producing, separating and extracting L-glutamine

A kind of glutamine and the obtained technology are applied in the field of L-glutamine production to achieve high oxygen solubility and improve the effect of fermentation yield

Active Publication Date: 2021-10-08
XINJIANG FUFENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, domestic medicinal L-glutamine mainly relies on imports, and the demand in the international market is strong. Since the glutamine products produced by the existing extraction process are difficult to meet the requirements of some customers, how to improve fermentation efficiency and reduce fermentation costs, And adopting the method of simulated moving bed chromatography to extract L-glutamine so as to meet the needs of customers and improve product yield and quality is a key problem that needs to be solved in the prior art

Method used

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  • A method for producing, separating and extracting L-glutamine
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  • A method for producing, separating and extracting L-glutamine

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Embodiment 1

[0032] A method for producing, separating and extracting L-glutamine, comprising the steps of:

[0033] Step 1) Fermentation: Corynebacterium glutamicum GM34 was used as the strain, see "Response Surface Method Optimization of L-Glutamine Fermentation Medium, Biotechnology Communications 2013".

[0034] Corynebacterium glutamicum seed solution (OD 600nm 13.1) Inoculate 10% inoculum into a 100L fermenter containing 50L fermentation medium, and the fermentation time is 48h; the fermentation time is divided into two stages, the first stage is 0-24h, and the fermentation temperature is 32°C. Add ammonia water to control the pH at 5.0; the second stage is 24-48h, the fermentation temperature is 32°C, and the pH is controlled at 7.0 by adding ammonia water. After the second stage is completed, the L-glutamine fermentation broth is obtained;

[0035] When the fermentation lasts to 24 hours, add the promoting solution to the fermentation medium at a rate of 0.05ml / min.L (0.05ml per m...

Embodiment 2

[0047] A method for producing, separating and extracting L-glutamine, comprising the steps of:

[0048] Corynebacterium glutamicum seed solution (OD 600nm 13.4) Inoculate 9% inoculum into a 100L fermenter containing 50L fermentation medium, and the fermentation time is 48h; the fermentation time is divided into two stages, the first stage is 0-24h, and the fermentation temperature is 32℃ Add ammonia water to control the pH at 5.0; the second stage is 24-48h, the fermentation temperature is 32°C, control the pH at 7.0 by adding ammonia water, and collect the fermentation broth after the second stage is completed;

[0049] When the fermentation lasts for 24 hours, add the promoting solution to the fermentation medium at a rate of 0.07ml / min.L (0.07ml per minute per liter of fermentation broth) until the end of fermentation;

[0050] During the whole fermentation process, the stirring speed is controlled at 200rpm, the dissolved oxygen is controlled at 25%, and the glucose conce...

Embodiment 3

[0061] 1. On the basis of Comparative Example 1, the effect of soybean oil addition in the fermentation medium on the production of L-glutamine was studied. Set the concentration of soybean oil to 0, 5, 10, 15, 20, 25, 30 respectively, the unit is ml / L, such as Figure 1-2 As shown, as the concentration of soybean oil increases, the concentration of bacteria and the fermentation yield of L-glutamine also increase. When it increases to 15ml / L, the increase of L-glutamine begins to slow down, and the concentration of soybean oil reaches 20ml / L , the production of L-glutamine was close to the peak value, compared with when no soybean oil was added, the production of L-glutamine increased by 13.7%, and the concentration of bacteria increased by 6.5%; if the concentration of soybean oil continued to increase, the effect on L-glutamine was small ; During the aerobic fermentation of Corynebacterium glutamicum to produce L-glutamine, the oxygen transfer from the gas phase to the liqui...

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Abstract

The invention belongs to the production field of L-glutamine, and discloses a method for producing, separating and extracting L-glutamine, which includes the following steps: 1) fermentation, 2) ceramic membrane filtration, 3) concentration and redissolution, 4) Simulated moving bed chromatography, 5) Activated carbon decolorization, 6) Refining concentration, crystallization and centrifugation, 7) Fluidized bed drying. The invention improves the output, purity, extraction yield, product quality and production efficiency of L-glutamine, and at the same time, reduces the amount of acid and alkali, reduces the discharge of waste water and waste, and reduces the pressure on environmental protection.

Description

technical field [0001] The invention belongs to the technical field of L-glutamine production, in particular to a method for producing, separating and extracting L-glutamine. Background technique [0002] L-glutamine (L-Glutamine), also known as α-aminoglutamic acid, glutamic acid-5-amide, is white crystal or crystalline powder, soluble in water, odorless, non-toxic, almost insoluble in methanol , ethanol, ether, benzene, acetone, ethyl acetate, chloroform and ethyl ethanol, etc., unstable in neutral solutions, easily decomposed into glutamic alcohol or propyl esterified into pyrrole carboxyl alcohol in alcohol, alkali or hot water , Odorless, slightly sweet. [0003] L-Glutamine is the most abundant amino acid in the human body and is a non-essential amino acid. Under normal circumstances, the glutamine synthesized in the human body can meet the metabolic needs, but in emergency and high metabolic states such as strenuous exercise, trauma, infection, etc., the content of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P13/02C07C231/24C07C237/06C12R1/15
CPCC07C231/24C12P13/02C07C237/06
Inventor 包鑫边恩来崔小红冯世红李江雷李江涛史静东
Owner XINJIANG FUFENG BIOTECH
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