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Granulation-free fermentation feed for penaeus orientalis

A technology for fermenting feed and prawns, applied to animal feed, animal feed, bacteria used in food preparation, etc., can solve the problem that aerobic bacteria do not fully play the role of bacteria, the quality of fermented products cannot be guaranteed, and pathogenic bacteria Or the increase of harmful bacteria and other problems, to achieve the effect of enhancing immunity, improving the structure of microbial flora, and reducing the feed coefficient

Pending Publication Date: 2020-04-28
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the fermentation process of prawn fermented feed is mainly to add bacillus, yeast, aspergillus and lactic acid bacteria to the existing prawn compound feed for anaerobic fermentation (anaerobic bag or plastic film sealing), this process mainly includes the following Question: 1. Bacillus, yeast and Aspergillus are aerobic bacteria. If direct anaerobic fermentation, these aerobic bacteria will not give full play to the role of the strain itself. Through aerobic fermentation of feed raw materials, a large amount of protease, Peptides and sugars, energy for later anaerobic fermentation
2. The existing compound pellet feed is not a sterile feed. Adding these strains for direct fermentation may bring in pathogenic bacteria or increase the number of harmful bacteria. or aquaculture water environment have potential disease-causing risks

Method used

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  • Granulation-free fermentation feed for penaeus orientalis
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  • Granulation-free fermentation feed for penaeus orientalis

Examples

Experimental program
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Effect test

Embodiment 1

[0031] (1) Preparation of fermentation strain

[0032] Prepare Bacillus subtilis, Saccharomyces cerevisiae, Lactobacillus plantarum, and Enterococcus faecalis seed liquid, and the number of viable bacteria is 1.0×10 9 More than cfu / mL;

[0033] a. Preparation of Bacillus subtilis seed liquid

[0034] Pick Bacillus subtilis and inoculate it into LB medium, and activate it on a shaking table at 37°C for 16 hours; then inoculate the activated bacterial liquid into the primary seed medium according to the mass ratio of 3% inoculum, and cultivate it on a shaking table at 37°C for 24 hours to obtain Bacillus subtilis seed liquid. The primary seed culture medium is: by mass fraction, including glucose 0.5%, cornstarch 1.5%, soybean meal 2%, yeast powder 0.5%, magnesium sulfate 0.2%, dipotassium hydrogen phosphate 0.2%, manganese sulfate, 0.03%, calcium carbonate 0.24%, the balance is water, pH7.0±0.2, and its preparation method is to mix the above-mentioned ingredients uniformly, ...

Embodiment 2

[0049] (1) Preparation of fermentation strain (same as Example 1)

[0050] Prepare Bacillus subtilis, Saccharomyces cerevisiae, Lactobacillus plantarum, and Enterococcus faecalis seed liquid, and the number of viable bacteria is 1.0×10 9 More than cfu / mL;

[0051] 2. Crushing, grading, sieving and mixing of fermented feed raw materials: crushing feed raw materials corn and soybeans to obtain crushed corn and soybeans respectively, and grading and sieving to obtain particles with a particle size of 0.5-0.9mm. Then take by weighing according to the following weight ratio: 20% of crushed corn, 40% of crushed soybeans, 20% of fish meal, 19% of bran, 1% of prawn premixed feed, add water until the moisture content is 40%, and mix evenly with a mixer to obtain solid media.

[0052] 3. Cooking and sterilizing solid medium, air cooling

[0053] The above-prepared solid medium was sterilized by high-temperature cooking at 121°C for 30 minutes, and cooled by an air cooler at 34°C for ...

Embodiment 3

[0063] 1, the preparation of fermentation strain (with embodiment 1)

[0064] Prepare Bacillus subtilis, Saccharomyces cerevisiae, Lactobacillus plantarum, and Enterococcus faecalis seed liquid, and the number of viable bacteria is 1.0×10 9 cfu / mL or more.

[0065] 2. Crushing, grading, sieving and mixing of fermented feed raw materials: crushing feed raw materials corn and soybeans to obtain crushed corn and soybeans respectively, and grading and sieving to obtain particles with a particle size of 0.5-0.9mm. Then take by weighing according to the following weight proportions: 26% of crushed corn, 32% of soybean crush, 20% of fish meal, 21.5% of bran, 0.5% of prawn premixed feed, add water until the moisture content is 40%, and mix evenly with a mixer to obtain solid media.

[0066] 3. Cooking and sterilizing solid medium, air cooling

[0067] The above-prepared solid medium was sterilized by high-temperature cooking at 121°C for 30 minutes, and cooled by an air cooler at 3...

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Abstract

The invention discloses a granulation-free fermentation feed for penaeus orientalis. The feed is prepared by the following method: crushing corn into crushed corn, and crushing soybeans into crushed soybeans; adding water into 20%-26% by mass of the crushed corn, 32-45% by mass of the crushed soybeans, 15-20% by mass of fish meal, 0.5%-1% by mass of penaeus orientalis pre-mixed feed and the balance of bran until the water content is 38-40% by weight, performing uniform mixing, and performing sterilization to obtain a solid culture medium; and inoculating bacillus subtilis, saccharomyces cerevisiae, lactobacillus plantarum and enterococcus faecalis in the solid culture medium, putting the solid culture medium in a sterile culture room, performing aerobic culture for 50-80 h at a constant temperature of 28-32 DEG C, after aerobic fermentation is completed, putting the product in an anaerobic bag, and performing anaerobic fermentation for 60-90 h at a constant temperature of 28-32 DEG C to obtain the granulation-free fermentation feed for the penaeus orientalis. Through the two-stage fermentation technology of first aerobic fermentation and then anaerobic fermentation, the method improves nutrient value of the feed and an utilization rate of the raw materials, improves a breeding water environment, promotes digestion, absorption and utilization of the feed by the penaeus orientalis, improves intestinal health, and improves the immunity and survival rate of the penaeus orientalis, and reduces use of antibiotics during breeding.

Description

technical field [0001] The invention belongs to the field of prawn feed, and in particular relates to a granulation-free fermented prawn feed. Background technique [0002] my country's shrimp farming industry has formed a complete industrial system. With the continuous expansion of industrial farming density and scale, various diseases occur frequently. Bacterial diseases caused by Vibrio have caused huge economic losses to the shrimp farming industry. At present It has seriously restricted the development of shrimp farming in my country and even in the world. Farmers usually use a large amount of antibiotics. Although they have achieved short-term results, they have led to increased drug resistance of pathogenic microorganisms, decreased diversity of microbial communities, drug residues, and deterioration of the breeding environment. After the feed is processed by microbial fermentation, it can not only improve its nutrient absorption level, reduce the content of toxic and...

Claims

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Application Information

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IPC IPC(8): A23K10/12A23K10/18A23K10/22A23K10/26A23K10/30A23K10/37A23K50/80C12N1/20C12N1/16C12R1/125C12R1/865C12R1/25C12R1/01
CPCA23K10/12A23K10/18A23K10/22A23K10/26A23K10/30A23K10/37A23K50/80C12N1/20C12N1/16A23V2400/169Y02A40/818Y02P60/87
Inventor 许国焕张丽成艳波谭文俊李志朋苏奎英袁满华
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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