A method for detecting the contents of paeoniflorin, liquiritin and ammonium glycyrrhizinate in Chashao oral liquid

A technology of Chai Shao oral liquid and ammonium glycyrrhizinate, which is applied in the field of quality inspection of Chinese veterinary medicine preparations, can solve problems such as long inspection time, unstable quality, and unstable therapeutic effect of traditional Chinese medicine compound

Active Publication Date: 2021-08-10
山东省兽药质量检验所
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  • Abstract
  • Description
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Problems solved by technology

[0003] Traditional Chinese medicine is a complex system composed of multiple components and factors. The instability and uncontrollability of its quality will directly lead to the instability and uncontrollability of the therapeutic effect of traditional Chinese medicine compound.
In the prior art, only paeoniflorin is detected for Chai Shao Oral Liquid by liquid chromatography, which takes a long time to detect and consumes a large amount of consumable mobile phase

Method used

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  • A method for detecting the contents of paeoniflorin, liquiritin and ammonium glycyrrhizinate in Chashao oral liquid
  • A method for detecting the contents of paeoniflorin, liquiritin and ammonium glycyrrhizinate in Chashao oral liquid
  • A method for detecting the contents of paeoniflorin, liquiritin and ammonium glycyrrhizinate in Chashao oral liquid

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Embodiment 1

[0036] Embodiment 1 A kind of detection method of content of paeoniflorin, liquiritin and ammonium glycyrrhizinate in Chai Shao oral liquid

[0037] 1 Materials and methods

[0038] 1.1 Drug and reagent reference substances are China National Institutes for Food and Drug Control: Paeoniflorin reference substance: batch number: 110736-201438, content 96.4%; ammonium glycyrrhizinate reference substance, batch number 110731-201720, content 97.7%; liquiritin reference substance, batch number 11610-201106, content 93.7%. Acetonitrile and phosphoric acid are chromatographically pure; ethanol is analytically pure; ultrapure water. Chai Shao Oral Liquid is provided by Qingdao Weilan Biological Co., Ltd., a new veterinary drug research and development cooperation unit of the 2018 provincial key research and development plan project.

[0039] 1.2 Instrument Analytical balance: Sensitivity 0.00001g; Waters Acquity TM Ultra performance LC ultra-high performance liquid chromatography; ...

Embodiment 2

[0072] Example 2 Detection method of paeoniflorin, liquiritin and ammonium glycyrrhizinate in Chai Shao oral liquid Chai Shao oral liquid includes the following components by weight:

[0073] 12 parts of Bupleurum, 4 parts of Paeoniae Alba, 10 parts of Citrus aurantium, and 8 parts of Licorice.

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Abstract

The invention relates to a method for detecting the contents of paeoniflorin, liquiritin and ammonium glycyrrhizinate in Chashao oral liquid, belonging to the field of quality detection of Chinese veterinary medicine preparations. The present invention adopts C 18 Chromatographic column, column temperature: 35°C; gradient elution with acetonitrile-0.4% phosphoric acid aqueous solution; flow rate: 0.35mL / min; injection volume: 1μL; detection wavelength: 238nm. Qualitative and quantitative detection of paeoniflorin, liquiritin and ammonium glycyrrhizinate were carried out by spectrogram, retention time and peak area parameters. The three have a good linear relationship in the range of 2-110 μg / mL; the detection limit of the method is 1 μg / mL, and the quantitative limit of the method is 2 μg / mL, which fully meet the detection requirements. The invention has good chromatographic separation, fast analysis speed, simple pretreatment, short detection time, and more than 90% of consumables and mobile phase can be saved.

Description

technical field [0001] The invention belongs to the field of quality detection of traditional Chinese veterinary medicine preparations, and relates to a detection method for the content of paeoniflorin, liquiritin and ammonium glycyrrhizinate, in particular to a detection method for the content of paeoniflorin, liquiritin and ammonium glycyrrhizinate in Chai Shao oral liquid . Background technique [0002] The new Chinese veterinary medicine Chai Shao Oral Liquid has the effect of clearing stagnant heat and harmonizing the liver and spleen. According to the relevant technical requirements of new veterinary drugs, pharmacy, pharmacology, toxicology and clinical research were carried out on it, and it is mainly used for the treatment of chicken liver damage caused by mycotoxins. It mainly contains pentacyclic triterpenoids, monoterpene glycosides, triterpene saponins and flavonoids, etc. It has many functions such as sedation, antipyretic and analgesic, anti-inflammatory and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 魏秀丽徐恩民张传津李有志杨志昆张会梅
Owner 山东省兽药质量检验所
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