ZIF nano-enzyme and preparation method and application thereof
A nano-enzyme, ZIF-90 technology, applied in the field of ZIF nano-enzyme and its preparation, can solve the problems of high cost, long time, poor stability of natural enzymes, etc., and achieve the effects of easy synthesis, low cost, low cost and no pollution
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Embodiment 1
[0029] A preparation method of ZIF nanozyme, the steps comprising:
[0030] 1) Preparation of copper nanoclusters (CuNCs):
[0031] 2.5 mL glutathione (GSH) aqueous solution (50 mM) with 2.5 mL CuSO 4 ·5H 2 O aqueous solution (10 mM) was fully mixed, then adjusted the pH of the mixed solution to 7 with 1 M NaOH aqueous solution under stirring, the mixed solution changed from a white suspension state to a clear and transparent state, and placed for use;
[0032] 2) Copper nanoclusters plus Al ion mixed solution (CuNCs+Al 3+ ) preparation:
[0033] Add 100 μL of Al ion aqueous solution (0.1 M) to the CuNCs solution synthesized above with pH=7, and stir for 5 minutes.
[0034] 3) Preparation of artificial nanozyme (CuNCs-GOx / ZIF-90) solution in which glucose oxidase was assembled into CuNCs / ZIF-90 nanocomposites:
[0035] Weigh 0.5960 g Zn(NO 3 ) 2 6H2O and 0.0961 g imidazole-2-carboxaldehyde (2-ICA) were dissolved in 20 mL of DMF solution, sonicated for 10–20 minutes unti...
Embodiment 2
[0040] A ZIF nanozyme is applied to the detection method of glucose in organisms, and the steps comprise:
[0041] (1) Acquisition of rat cerebral cortex microdialysate: All animal studies involved in this work were first carried out in accordance with the Laboratory Animal Care and Use Guidelines of the National Center for Nanoscience and Technology. First, 250-300 g adult male rats were fed water and drink according to the day-night time of 12:12 hours; then, the anesthetized rats were fixed on a stereotaxic frame, and microdialysis was carried out through a standard stereotaxic system. Catheters (BAS / MD-2250) were implanted in the cerebral cortex, and the body temperature of the rats was maintained at 37 ° C; The postoperative rats were recovered for at least 24 hours. Next, the striatum of the rats was implanted with a microdialysis probe (dialysis length 4 mm, diameter 0.24 mm) and was continuously perfused at a rate of 2.0 μL / min Artificial cerebrospinal fluid aCSF was ...
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