Clostridium kogasensis EO-08 and application thereof in degrading ethylene oxide

An ethylene oxide, carboxyl-like technology, applied in the field of biodegradation, can solve the problems of easy explosion, low absorption saturation, high biological toxicity, etc.

Inactive Publication Date: 2020-05-15
CHINESE RES ACAD OF ENVIRONMENTAL SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are two main methods for industrial sterilization treatment of ethylene oxide: one is to use sulfuric acid as the reaction substrate for neutralization reaction, the disadvantage of this technology is that the absorption saturation is small, and it will produce side effects that are difficult to handle. Product increases processing costs
The other is to use an oxidation reaction furnace for oxidation reaction. This technology requires very high control of technical parameters, and the process is prone to explosion.
[0004] Microbial degradation is a possible solution. However, as a fungicide, ethylene oxide is highly toxic to organisms, and the microenvironment of the microbial degradation pool is very complex and affected by many factors.

Method used

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  • Clostridium kogasensis EO-08 and application thereof in degrading ethylene oxide
  • Clostridium kogasensis EO-08 and application thereof in degrading ethylene oxide
  • Clostridium kogasensis EO-08 and application thereof in degrading ethylene oxide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Enrichment, screening and identification of EO-08 original strain

[0069] Weigh 10.0 g of sludge sample, add 100 mL of 0.03 mol / L phosphate buffer solution, mix and clarify for 120 min, and remove large particles of sediment. Take 1mL of the suspension and add 10mL of enrichment enrichment liquid medium (containing preferably 100mg / L low-concentration ethylene oxide), and place it on a shaker for anaerobic enrichment culture at 200r / min, anaerobic at 37°C Cultured for 24-48h to observe the growth.

[0070]Bacteria enrichment medium: tryptone 17g, soybean peptone 3g, sodium chloride 5g, dipotassium hydrogen phosphate 2.5g, glucose 2.5g, distilled water 1000ml, adjust pH to 7.0-7.5, pack into 500mL Erlenmeyer flask, each Erlenmeyer flask 250mL. Sterilize at 121°C for 20 minutes, cool to room temperature, take out the ice box from the refrigerator, and place reagent pure ethylene oxide on the ice box. Use a sealed syringe to draw 28 μL of ethylene oxide liquid into the...

Embodiment 2

[0086] Ethylene oxide-induced domestication of strain EO-08

[0087] Ethylene oxide tolerance induced acclimatization: Using the streak method, inoculate the EO-08 original strain in the 100mg / L ethylene oxide tolerance acclimatization medium, inoculate the culture plate and the 2.5L anaerobic gas generating bag at the same time Place in a 2.5L round-bottom vertical anaerobic culture bag, seal the culture bag, and place it in a 37°C incubator for anaerobic culture for 48 hours; select the single colony with the largest colony radius on the plate, and subculture until it contains 200mg / L ethylene oxide Alkane tolerance acclimation medium, 37°C constant temperature anaerobic culture for 48 hours; select the single colony with the largest colony radius on the plate, subculture to 500 mg / L ethylene oxide tolerance acclimation medium, 37°C constant temperature anaerobic culture for 48 hours; Select the single colony with the largest colony radius on the plate, subculture to the 800...

Embodiment 3

[0095] Identification of Ethylene Oxide Degradation Effect of EO-08 Strains

[0096] Microbial culture and activation: Take out EO-08 strains and EO-08 original strains from the -80°C refrigerator, take 10 μL and inoculate in 100 mL Sabouraud liquid medium, and culture anaerobically at 37°C, 200 rpm for 48 hours. The number of bacteria in the culture solution is 10 10 ~10 12 cfu / mL.

[0097] Sabouraud liquid medium: Glucose 40g, casein trypsin digest, animal tissue gastric enzyme digest equal mixture 10g, pH 5.4-5.8, distilled water 1000mL, divided into 500mL Erlenmeyer flasks, each Erlenmeyer flask 250mL . Sterilize at 121°C for 20 minutes and cool to room temperature.

[0098] Sabouraud's liquid induction medium: 10g of peptone, 12g of glucose, 1000mL of distilled water, aliquoted into 400mL, sterilized at 121°C for 20min, cooled to room temperature and stored. Before use, add 160 mg of ethylene oxide with a sealed syringe to make a liquid medium containing a carbon sou...

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Abstract

The invention discloses clostridium kogasensis EO-08 and an application thereof in degrading ethylene oxide, and relates to the technical field of biodegradation in an ethylene oxide treatment technology. The preservation number of the clostridium kogasensis EO-08 is CGMCC No. 18438. The invention also dicloses the application of the clostridium kogasensis EO-08. According to the invention, the problem of sewage disposal emission in industrial ethylene oxide post-treatment measures is solved; microorganisms for degrading the ethylene oxide are effectively screened out; and the degrading effectof degrading bacteria is improved. The EO-08 can degrade the ethylene oxide with a concentration of 400 mg / L under a condition of a carbon source with a concentration of 30%, and the degradation rateof the ethylene oxide can reach 83.61%; and under the condition of the carbon source with the concentration of 30%, the ethylene oxide with a high concentration of 800 mg / L can be degraded, and the degradation rate of the ethylene oxide can reach 91.70%.

Description

technical field [0001] The invention relates to the technical field of biodegradation in ethylene oxide treatment technology, in particular to the technical field of screening and application of ethylene oxide degrading bacteria dissolved in liquid, more specifically to a carboxy-shaped bacterium EO- 08 and its application in the degradation of ethylene oxide. Background technique [0002] At present, ethylene oxide is one of the important petrochemical products in the modern chemical industry. Ethylene oxide has an irreplaceable position in the medical sterilization industry due to its small molecular weight, high penetrability, and its ability to undergo coupling reactions with biological macromolecules such as DNA and proteins. In addition, the cost of ethylene oxide sterilization is low, and it can be used for industrial-scale large-scale sterilization. It is one of the most important low-temperature sterilizers so far. However, ethylene oxide is extremely active, flam...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/36C02F3/34C12R1/145C02F101/34
CPCC02F3/34C02F2101/34C12N1/36C12N1/205C12R2001/145
Inventor 薛建龙刘征涛何业成侯东新熊贵贵王晓南徐倩云彭颖陈燕玲
Owner CHINESE RES ACAD OF ENVIRONMENTAL SCI
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