Lactic acid bacteria crystal composition with functions of promoting intestinal stem cell proliferation, anti-virus, anti-inflammatory and anti-allergy and preparation method thereof

The technology of lactic acid bacteria and the composition is applied in the field of lactic acid bacteria crystal composition and its preparation field with the effects of promoting intestinal stem cell proliferation, anti-virus, anti-inflammatory and anti-allergic, and can solve the problem of repairing mucosal tissue without probiotics, high cost, solvent residues Toxicity etc.

Active Publication Date: 2020-05-26
BIOZYME BIOBTECH CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In another study on algae, the polysaccharides and polyphenols in algae have anti-oxidation, anti-cancer, anti-coagulation, anti-inflammation, anti-virus, anti-hyperlipidemia and other effects. In order to obtain these active substances, the industry usually uses hot water, Solvent, acid hydrolysis, enzyme hydrolysis and other extraction methods, but these extraction methods are usually accompanied by some limiting factors, including: high cost, complicated process and residual toxicity of solvents
Another study found that the algae fermentation process can release mor...

Method used

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  • Lactic acid bacteria crystal composition with functions of promoting intestinal stem cell proliferation, anti-virus, anti-inflammatory and anti-allergy and preparation method thereof
  • Lactic acid bacteria crystal composition with functions of promoting intestinal stem cell proliferation, anti-virus, anti-inflammatory and anti-allergy and preparation method thereof
  • Lactic acid bacteria crystal composition with functions of promoting intestinal stem cell proliferation, anti-virus, anti-inflammatory and anti-allergy and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1: the fermentation process of algae fermentation liquid

[0042] Magnesium and calcium are added to the algae fermentation liquid during the fermentation process. After 540 days, 4 strains of yeast, 5 strains of lactic acid bacteria, and 3 strains of acetic acid bacteria are fermented by a total of 12 strains of bacteria to convert magnesium and calcium into an organic state, and then add lactic acid bacteria. The algae fermentation broth is coated on the outer layer of lactic acid bacteria to provide bioavailability.

[0043] The production process of the algae fermentation liquid of the present invention is divided into three stages of implanting strains, a total of four fermentation stages, stage 1: primary extraction fermentation, after pressing the algae, add 1 strain of yeast Saccharomyces cerevisiae for 90 days of primary extraction fermentation; stage 2 : compound fermentation stage, add 3 strains of yeasts such as: Saccharomycopsis fibufigera, Pichi...

Embodiment 2

[0044] Embodiment 2: the method for preparing the composition of algae fermentation broth coated in the shell of lactic acid bacteria crystal

[0045] The present invention prepares the method for the composition that algae fermented liquid is coated on the shell of lactic acid bacteria crystal, step 1: mixing step, the algae fermented liquid that has finished fermentation process and 1.5% four kinds of lactic acid bacteria solutions (such as: Lactobacillus rhamnosus, Lactobacillus plantarum, Lactobacillus acidophilus, Lactobacillus sporogenes equal proportion mixture) mixing and step 2: high-speed emulsification step, after the mixed solution is emulsified by a high-speed emulsification head, the colloidal structure of the algae fermentation liquid that has completed the fermentation process is coated on the outer layer of the lactic acid bacteria to form lactic acid bacteria crystals The shell completes the composition of lactic acid bacteria crystals. The lactic acid bacter...

Embodiment 3

[0046] Embodiment 3: acid resistance and choline resistance test of lactic acid bacteria crystal composition

[0047] Divide the acid resistance and cholinergic resistance tests into two groups:

[0048] (1) Four strains of lactic acid bacteria mixture,

[0049] (2) Four strains of lactic acid bacteria + algae fermentation liquid crystal composition

[0050] 1. Acid resistance test

[0051] Culture four strains of lactic acid bacteria in MRS medium respectively, and after culturing at 37°C for 48 hours, take 0.1ml of bacterial solution and mix them, add 5N HCl to adjust the pH value to 2, culture at 37°C for four hours, serially dilute and smear the plate, and cultivate Count the colonies after 24 hours; in addition, culture the crystallization composition of four strains of lactic acid bacteria + algae fermentation liquid in MRS medium, take 0.4ml after culturing at 37°C for 48 hours, add 5N HCl to adjust the pH value to 2, and culture at 37°C for four After 1 hour, serial...

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Abstract

The invention provides a lactic acid bacteria crystal composition coated with algae fermentation liquid with acid and choline resistance, which has the functions of promoting intestinal stem cell proliferation, antivirus, anti-inflammatory and anti-allergy, and a preparation method thereof.

Description

technical field [0001] The present invention provides a lactic acid bacteria crystal composition coated with algae fermentation liquid, which is produced in a pure natural way, using the algae fermentation liquid produced by fermentation as a colloid source to protect the bacteria, so that the composition has the ability to resist gastric acid and bile, and This composition uses a variety of lactic acid bacteria as the main body, which can provide the benefits of a variety of beneficial bacteria at one time. At the same time, the colloid of algae fermentation liquid can cooperate with lactic acid bacteria, and has the effect of promoting intestinal stem cell proliferation, anti-virus, anti-inflammation and anti-allergic effects. Lactic acid bacteria crystal composition and its preparation method. Background technique [0002] Lactic acid bacteria have positive effects on the human body. From 1961 to 1998, a total of 143 human clinical trials of lactic acid bacteria have been...

Claims

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Application Information

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IPC IPC(8): A61K36/04A61K9/50A61K35/744A61P31/12A61P29/00A61P17/00A61P37/08A61P11/02A61K35/748A61K36/02A61K36/05
CPCA61K9/5015A61K35/744A61P31/12A61P29/00A61P17/00A61P37/08A61P11/02A61K36/04A61K2236/19A61K36/05A61K36/02A61K35/748
Inventor 黄柽赖怡君赖柏儒
Owner BIOZYME BIOBTECH CORP
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