Preparation for controlling ovulation of equus animals and method for controlling ovulation
A preparation and animal technology, applied in the field of animal breeding, can solve the problems of soaring international donkey skin prices, the difficulty of applying the method for controlling ovulation of mares to female donkeys, etc., and achieve the effects of low production cost, low price of raw materials, and improved pregnancy rate.
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Embodiment 1
[0047] The preparation of the ovulation fluid of embodiment 1 control animal ovulation
[0048] 1. Ovulation fluids containing different sustained-release carriers and their application effects
[0049] (1) Preparation of ovulation fluid containing different slow-release carriers
[0050] The following A~J respectively provide the ovulation fluid containing different slow-release carriers in this embodiment:
[0051] A. Weigh 0.1g of deserelin and 2.0g of trehalose, add ethanol to fully dissolve to obtain a gonadotropin-releasing hormone solution; weigh 700g poly(DL-lactide-co-glycolide), add ethanol to fully Dissolve to obtain a slow-release carrier solution; mix the two solutions evenly, make up to 1000 g with ethanol, and obtain 0.01% deserelin ovulation solution.
[0052] B. Weigh 0.1g of deserelin and 2.0g of trehalose, add ultrapure sterile water to fully dissolve to obtain a gonadotropin-releasing hormone solution; weigh 700g of polysucrose, add ultrapure sterile wate...
Embodiment 2
[0098] The ovulation control of embodiment 2 Akhate gold (sweat blood) horse and the effect of natural mating
[0099] Near Hohhot, Inner Mongolia, 32 Ahatekin mares aged 3 to 10 years old were selected, with moderate body condition, healthy and normal fecundity. They were raised in pens with an outdoor playground, had free access to water, and were fed green hay (leymus chinensis and alfalfa) twice. From April to June, test the stallion with the stallion every morning from 8:00 to 10:00. If the stallion stands still after approaching, lifts its tail, opens its vulva frequently, and discharges urine, it means that the mare is ready for breeding. At the same time, use a B-ultrasound probe to check the follicles and uterine edema across the rectal wall. On the B-ultrasound machine, the "cross method" is used to measure the diameter of the follicle, that is, measure a maximum value in the longitudinal direction, then cross vertically, measure a maximum value in the horizontal di...
Embodiment 3
[0102] Example 3 Ovulation control of Mongolian horses and the effect of using frozen semen for breeding
[0103] In the vicinity of Shenyang City, Liaoning, 35 mares of Mongolian horses aged 3 to 8 were selected, with moderate fat condition, healthy and normal fecundity. They were reared in pens with an outdoor sports field, free to drink water, and fed green hay (leymus chinensis and alfalfa) twice. From June to August, from 8:00 to 10:00 in the morning, B-ultrasound rectal examination was used to check the development of follicles in the horse herd. On the B-ultrasound machine, the "cross method" is used to measure the diameter of the follicle, that is, measure a maximum value in the longitudinal direction, then cross vertically, measure a maximum value in the horizontal direction, add the two values and take the average value, which is the diameter of the follicle. When the diameter of the follicle reaches more than 35mm and the edema of the endometrium is obvious, the ...
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