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Culture medium capable of improving potassium dissolving activity of silicate bacteria and culture method

A technology of silicate bacteria and culture method, applied in the directions of microorganism-based methods, bacteria, biochemical equipment and methods, etc., can solve the problems of limited promotion and application, unstable potassium solution effect, and small absolute potassium solution, etc. To achieve the effect of promoting accumulation, improving potassium solution ability, and reasonable compounding

Pending Publication Date: 2020-05-29
卜树华
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] At present, people have conducted extensive research on the screening of high-efficiency silicate bacteria, the determination of potassium-dissolving ability, the mechanism of potassium-dissolving action, and the breeding of excellent strains, but there are still many problems in practical applications, such as the identification of silicate bacteria. The absolute amount of potassium solution is still very small, the efficiency of potassium solution is low, the speed of potassium solution is slow, and the effect of potassium solution is unstable, etc., which greatly limits its promotion and application

Method used

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  • Culture medium capable of improving potassium dissolving activity of silicate bacteria and culture method
  • Culture medium capable of improving potassium dissolving activity of silicate bacteria and culture method

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Bacteria used: Bacillus colioids ( Bacillus mucilaginosus Krassilnikov ), the strain number is ACCC19749; Bacillus circulans ( Bacillus circulans ), and the strain number is ACCC 03263, all purchased from the China Agricultural Microorganism Culture Collection Management Center.

[0024] Medium used: soluble starch 10.0g / L, mannan oligosaccharide 5.0g / L, yeast extract 2.0g / L, Na 2 HPO 4 1.0g / L, MgSO 4 ·7H 2 O 0.13g / L, CaCO 3 3.0g / L, NaCl 0.3g / L, ferric ammonium citrate 20mg / L, MnSO 4 ·H 2 O11mg / L, CuSO 4 0.3mg / L, NaMoO 4 2H 2 O 0.24mg / L, green algae powder 6.0g / L, tryptophan 150mg / L, isopentenyl adenine 5.5mg / L, anisole 18μmol / L, N-methyl-N-nitro-N- Nitroguanidine 11μmol / L, yttrium nitrate 75mg / L, alfalfa extract 8ml / L, soybean oil 2ml / L, and the initial pH value was adjusted to 7.2.

[0025]Wherein the preparation method of the alfalfa extract comprises: washing the stems and leaves of the alfalfa, soaking them in sodium hydroxide solution for 15 minutes, t...

Embodiment 2

[0029] The medium used in this embodiment: soluble starch 5.0g / L, mannan oligosaccharide 3.0g / L, yeast extract 1.0g / L, Na 2 HPO 4 0.5g / L, MgSO 4 ·7H 2 O 0.05g / L, CaCO 3 2.0g / L, NaCl 0.1g / L, ferric ammonium citrate 15mg / L, MnSO 4 ·H 2 O9mg / L, CuSO 4 0.2mg / L, NaMoO 4 2H 2 O 0.15mg / L, Chlorella powder 4.5g / L, Tryptophan 120mg / L, Isopentenyl adenine 4.0mg / L, Anisole 14μmol / L, N-methyl-N-nitro-N- Nitroguanidine 7μmol / L, yttrium nitrate 50mg / L, alfalfa extract 6ml / L, soybean oil 1ml / L, and the initial pH value was adjusted to 7.2.

[0030] The cultivation method was the same as that in Example 1, and the fermentation broth of Bacillus colloidus and Bacillus circulans were obtained respectively.

Embodiment 3

[0032] The medium used in this embodiment: soluble starch 15.0g / L, mannan oligosaccharide 7.0g / L, yeast extract 3.0g / L, Na 2 HPO 4 1.5g / L, MgSO 4 ·7H 2 O 0.2g / L, CaCO 3 4.0g / L, NaCl 0.5g / L, ferric ammonium citrate 25mg / L, MnSO 4 ·H 2 O13mg / L, CuSO 4 0.4mg / L, NaMoO 4 2H 2 O 0.33mg / L, Chlorella powder 7.5g / L, Tryptophan 180mg / L, Isopentenyl adenine 7.0mg / L, Anisole 22μmol / L, N-methyl-N-nitro-N- Nitroguanidine 15μmol / L, yttrium nitrate 100mg / L, alfalfa extract 10ml / L, soybean oil 3ml / L, and the initial pH value was adjusted to 7.2.

[0033] The cultivation method was the same as that in Example 1, and the fermentation broth of Bacillus colloidus and Bacillus circulans were obtained respectively.

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Abstract

The invention provides a culture medium capable of improving potassium dissolving activity of silicate bacteria and a culture method. The culture medium is composed of the following components: soluble starch, molasses, mannan oligosaccharides, soya bean meal, chlorella powder, Na2HPO4, MgSO4.7H2O, CaCO3, NaCl, ammonium ferric citrate, MnSO4.H2O, CuSO4, NaMoO4.2H2O, tryptophan, n6-(delta 2-isopentenyl)-adenine, anisole, 1-methyl-3-nitro-1-nitrosoguanidine, yttrium nitrate, an alfalfa extract and soybean oil. According to the culture medium capable of improving the potassium dissolving activityof the silicate bacteria and the culture method, the silicate bacteria culture medium is formed through a specific formula, in the formula of the silicate bacteria culture medium, the components interact, not only can growth and reproduction of the silicate bacteria be promoted, but also the potassium dissolving activity of the silicate bacteria can be effectively improved, after culture is conducted, the efficiency of the silicate bacteria of dissolving potassium of potassium-bearing minerals (potassium feldspar and mica) is significantly improved, and a basis is provided for subsequent development of potassium dissolving microbial agents and microbial fertilizers.

Description

technical field [0001] The invention relates to a culture medium and a culture method capable of improving the potassium-dissolving activity of silicate bacteria, belonging to the field of biotechnology. Background technique [0002] Potassium is one of the three major nutrients necessary for plant growth and development. Potassium accounts for 0.2% to 4.1% of the dry matter content in plants, second only to nitrogen. In the process of plant growth and development, potassium can maintain the osmotic pressure of plant cells, activate the activity of various enzymes in plants, promote photosynthesis, increase the assimilation rate of carbon dioxide, and promote the synthesis and transport of substances in plants. Potassium can also enhance the stress resistance of plants such as cold resistance, drought resistance, high temperature resistance, disease resistance, salt resistance, and lodging resistance, thereby improving crop yield and quality. The chemical forms of potassium...

Claims

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Application Information

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IPC IPC(8): C12N1/38C12N1/20C12R1/07C12R1/09
CPCC12N1/38C12N1/20
Inventor 卜树华
Owner 卜树华
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