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Preparation method of fluorescent probe test paper for detecting lead content of soil

A fluorescent probe and lead content technology, which is applied in soil testing, measuring devices, fluorescence/phosphorescence, etc., can solve the problems of insufficient detection precision and high detection limit of the test paper method, and achieve easy operation, easy portability, and anti-interference good sex effect

Inactive Publication Date: 2020-06-02
广州市尚信净化工程有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The organic small molecule fluorescent probe test paper of the present invention has the advantages of simple manufacture, low detection limit, sensitive response and high efficiency, and solves the shortcomings of insufficient detection precision and high detection limit of the test paper method on the market

Method used

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  • Preparation method of fluorescent probe test paper for detecting lead content of soil
  • Preparation method of fluorescent probe test paper for detecting lead content of soil
  • Preparation method of fluorescent probe test paper for detecting lead content of soil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Synthesis of Embodiment 1 Organic Fluorescent Small Molecules

[0024] (1) Dissolve 8-hydroxyquinoline and hexamethylenetetramine in a methanol solvent in a molar ratio of 1:2, and react at reflux at 70°C for 2 hours. After cooling, rotary evaporation under reduced pressure is performed, and purified by chromatography to obtain 5,7-Dialdehyde-8-hydroxyquinoline.

[0025] (2) Dissolve 3-iodopropane-1-ammonia and 2-methylbenzothiazole in n-butanol at a molar ratio of 3:1, and react in a sealed high-pressure bottle at 60°C for 2-6 hours to obtain a yellow color The solid 3-(3-aminopropyl)-2-methylbenzothiazole was filtered, washed twice with 1 equivalent of diethyl ether, and then dried. The yield was above 98%.

[0026] (3) React 5,7-dialdehyde-8-hydroxyquinoline and twice the amount of 3-(3-aminopropyl)-2-methylbenzothiazole in n-butanol system, add organic base Triethylamine is the condensation catalyst. React for 2-6 hours at 120°C. When the system starts to condense...

Embodiment 2

[0027] Example 2 is a graph showing the titration of heavy metal lead ions by small organic molecule fluorescent compounds in Tris-HCl.

[0028] The 5mM organic small molecule fluorescent compound stock solution of the present invention is diluted to a concentration of 5 μM, and then heavy metal lead ions of different concentrations are added and measured by a fluorescence spectrophotometer (slit width=10, scanning speed=200nm, Ex=526nm) Its respective fluorescence intensity, the titration solution is Tris-HCl solution, finds that this organic small molecule fluorescent compound and heavy metal lead ion have very strong fluorescent effect. After the organic small molecule fluorescent compound is combined with the heavy metal lead ion, the fluorescence intensity increases significantly with the increase of the metal concentration, and when the concentration reaches saturation, the concentration will no longer increase, such as figure 2 shown. However, when organic small molec...

Embodiment 3

[0029] Example 3 is a graph showing the titration of heavy metal lead ions by small organic molecule fluorescent compounds in acetone.

[0030] The 5mM organic small molecule fluorescent compound stock solution of the present invention is diluted to a concentration of 5 μM, and then heavy metal lead ions of different concentrations are added and measured by a fluorescence spectrophotometer (slit width=10, scanning speed=200nm, Ex=526nm) Their respective fluorescence intensity, the titration solution is an acetone solution, and it is found that the organic small molecule fluorescent compound and the heavy metal lead ion have a strong fluorescent effect. After the organic small molecule fluorescent compound is combined with the heavy metal lead ion, the fluorescence intensity increases significantly with the increase of the metal concentration. When the local concentration reaches saturation, the concentration no longer increases, but the fluorescence intensity is about 40% lower...

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Abstract

The invention relates to a preparation method of fluorescent probe test paper for detecting the lead content of soil, and the fluorescent probe test paper provided by the invention comprises an organic small molecular fluorescent material as shown in a formula (I), the fluorescent probe test paper disclosed by the invention is obviously distinguished under different concentrations of heavy metal lead ions, can be used for semi-quantitatively detecting the lead ions in sewage, cosmetics, foods and the like, and has substantive significance; the fluorescent probe test paper for detecting the heavy metal lead ions has the advantages of simple preparation, low detection limit, sensitive reaction, high efficiency and the like.

Description

technical field [0001] The invention relates to a method for preparing a fluorescent probe test paper for detecting lead content in soil, belonging to the field of soil detection. Background technique [0002] Lead is relatively common in minerals. The mining of mines and the burning of coal and oil are the main sources of lead pollution. In addition, pesticides, herbicides, batteries, pigments, dyes, electroplating and pipes, etc. will also cause lead pollution. . About 400,000 tons of lead are discharged into the environment in various ways every year in the world, causing irreversible pollution to the environment. Soil has a strong adsorption effect on lead. Whether it is lead desorbed in the air or lead carried by fertilizer, feces, and sludge, it is easily absorbed by the soil on the soil surface and the cultivated layer. Soil lead pollution indirectly causes damage to plants. Pollution, vegetables are an effective way for lead in the soil to accumulate to the human b...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N33/24C07D417/14C09K11/06
CPCG01N21/643G01N33/24C07D417/14C09K11/06G01N2021/6443C09K2211/1037C09K2211/1029
Inventor 李金杰
Owner 广州市尚信净化工程有限公司
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