In-vitro liver function detection method and system

Abstract

The invention relates to the technical field of medicine diagnosis and in particular relates to an in-vitro liver function detection method and system. The method comprises the following steps: a) respectively accessing arteries, veins and biliary tracts of a liver into related pipelines of an in-vitro liver mechanical perfusion apparatus, wherein the pipelines are filled with whole blood in advance; b) injecting a contrast medium into a hepatic portal vein to implement circulation, and testing T1 relaxation time of discharged bile by using a low-field magnetic resonance imaging analyzer; andc) comparing variation situations of the T1 relaxation time of the discharged bile with a reference value, and judging that if the T1 relaxation time of a same time point is longer than the referencevalue, it means that the metabolic function of the liver is lower that a liver corresponding to the reference value, and otherwise, it means the metabolic function of the liver is better than that ofthe liver corresponding to the reference value. The method makes up the limitation that liver functions cannot be directly reflected by conventional detection indexes.

Description

technical field [0001] The invention relates to the technical field of medical diagnosis, in particular to a method and system for detecting the function of an isolated liver. Background technique [0002] Liver transplantation is a gradually mature medical technology. The process includes: after the donor (donor) donates the liver, the liver is taken out and stored in a refrigerator and transported to the transplant medical center, and then the liver is surgically transplanted into the patient (recipient). Donor livers inevitably experience cold and warm ischemic injury during acquisition, storage, and transportation. A certain degree of cold and warm ischemic injury will lead to problems such as graft failure and biliary complications; other livers come from donors who are elderly or have fatty liver, which will seriously affect the postoperative survival and prognosis of transplanted patients. Quality assessment of donor livers prior to transplantation is critical. The...

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Problems solved by technology

However, the current application of these contrast agents is limited to improving the imaging contrast between liver tissue and tumor tissue during MRI imaging, and is mostly used to detect small solid tumors in the liver.

[0005] The following obstacles still exist in the liver function test of isolated liver donors: (1) Most of the in vivo liver function test indicators cannot directly reflect the metabolic function of the isolated liver. No direct relationship; (2) The indicators in the in vitro detection of perfusate have large individual differences, and the deviation from normal physiological indicators is large, so it is difficult to quantitatively evaluate the isolated liver function. The pH and blood glucose levels in the perfusate vary greatly among individuals, and static values ​​cannot be used to reflect liver function; (3) The existing biochemical detection methods for excreted bile from the isolated liver are low in sensitivity and poor in stability, and cannot quickly and accurately evaluate liver function

For example, using the traditional in vivo liver function assessment method indocyanine green bile excretion test to detect isolated liver function, the stability and sensitivity are low

(4) At present, the survival time of the liver in vitro is short, and there is a lack of suitable perfusion mode and mature detection process

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