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Long-acting composite enzyme disinfection composition for children and application thereof

A composite enzyme and composition technology, applied in the fields of biotechnology and medical and health care, can solve the mechanism of action, side reactions have not been clinically verified, biological enzyme preparations cannot achieve sustained-release long-acting antibacterial, and yeast and fungal contamination cannot be achieved. Control and other problems to achieve the effect of no skin coloring problems, strong durability and prolonged activity

Pending Publication Date: 2020-06-19
亘元(天津)生物医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these patents have certain practicability for antibacterial disinfection, they all face the same problem. The biological enzyme preparation cannot realize the functions of slow-release agent and long-acting antibacterial. and fungal contamination cannot be controlled
There are many kinds of Chinese herbal medicines, but their mechanism of action and side effects have not been clinically verified or hygienically evaluated.

Method used

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  • Long-acting composite enzyme disinfection composition for children and application thereof
  • Long-acting composite enzyme disinfection composition for children and application thereof
  • Long-acting composite enzyme disinfection composition for children and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Preparation of Complex Enzyme Inclusion Complex

[0024] In order to easily distinguish the groups of inclusion complexes, lysozyme in various compound enzyme dextrin inclusion complexes is abbreviated as A, lysozyme as B, chitinase as C, and helicase as D.

[0025] Preparation method of ABCD-type compound enzyme dextrin inclusion compound: Accurately weigh 2g of lysozyme (20000U / mg), 2mg of lysoglucoccus enzyme (1200U / mg), 2g of chitinase (100U / mg), and 0.2g of helicase , add 200ml of purified water to fully dissolve. Add it dropwise into 400ml of 4% hydroxypropyl β-cyclodextrin solution at a speed of 20ml / min, stir magnetically at 300rpm for 5min at room temperature, and ultrasonically for 10s, repeat the above steps 2 to 3 times, and transfer to vacuum rotary evaporation In the instrument, adjust the temperature to 40-60°C, and continue to rotate and stir for 4-10h. Transfer to a vacuum lyophilizer for lyophilization. The ABCD type compound enzyme dextr...

Embodiment 2

[0032] Example 2: Verification of the stability of complex enzyme inclusion complex

[0033] Select the AC-type compound enzyme in Example 1 and the AB-type compound enzyme in Comparative Example 1 as the experimental object, configure 0.05% solution, and simultaneously use the same activity of the unincluded AB and AC composition (marked as AB' and AC') The solution was used as a reference, and the activity of lysozyme in the above combination was used as an index to consider the stability of the compound enzyme in the inclusion compound. The above compound enzyme solutions were placed in an environment of 40°C for accelerated aging for 129 days, and the activities of the two solutions were measured respectively (equivalent to 2 years of storage at room temperature), and each group of experiments was repeated twice. The comparison is shown in Table 1.

[0034] Table 1 Stability test of inclusion compound

[0035]

[0036] It can be confirmed through tests that the comple...

Embodiment 3

[0037] Embodiment 3: verification of disinfection effect

[0038] Take Staphylococcus aureus and Candida albicans respectively as the representatives of clinical pathogenic microorganisms to measure the disinfection effect verification of the present invention. Separately configure the bacterial suspensions of Staphylococcus aureus and Candida albicans so that the concentration of the bacterial suspensions is 1-10×10 6 cfu / mL, cultured after serial dilution was used as a positive control, and a blank plate was used as a negative control. Get respectively 1g various types of compound enzyme dextrin inclusion complex ABCD, ABC, AC, AD or BC in embodiment 1, or 1g AB type compound enzyme dextrin inclusion compound, polyhexamethylene biguanide (PHMB ) 1g (20% w / w), trehalose 1g, add 1000g of purified water, mix well and make a spray form, that is, become a disinfectant spray ABCD, ABC, AC, AD, BC, AB, at the same time only add PHMB and The solution of the stabilizer was used as ...

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Abstract

The invention relates to a long-acting composite enzyme disinfection composition for children and an application thereof. The disinfection composition consists of a composite enzyme dextrin inclusioncompound, a positively charged cationic polymerization group, a stabilizing agent, a humectant and the like, wherein the composite enzymes comprise one or two of lysozyme and lysostaphin, and one or two of chitinase and snailase; and in each gram of the composite enzyme dextrin inclusion compound, the concentration of the lysozyme is 100-200mg / g, the concentration of the lysostaphin is 0.1-1mg / g,the concentration of the chitinase is 100-200mg / g, and the concentration of the snailase is 10-100mg / g. The long-acting composite enzyme disinfection composition for children can for be released slowly for resisting bacteria, can prolong activity and stress resistance of the composite enzymes in a disinfection product in a use environment, and can realize a long-acting antibacterial effect. In addition, the composition has the advantages of wide sterilization spectrum, small skin irritation and no skin coloring problem, has high durability, and can be suitable for both of adults and children with delicate skins.

Description

technical field [0001] The invention belongs to the fields of biotechnology and medical care, and in particular relates to a children's long-acting compound enzyme disinfection composition and its application. Background technique [0002] At present, clinical bacterial infections are quite serious, and more and more bacteria can resist antibiotic treatment, thus posing a serious threat to public health. At present, chemical therapeutic agents, mainly antibiotics, are widely used clinically. However, with the widespread use of antibiotics, various pathogenic microorganisms have shown significant drug resistance. Therefore, the medical community has been looking for a bactericidal agent that can replace antibiotics and have a bactericidal effect, but is not easy to produce drug-resistant strains. [0003] In addition, the public is mainly involved in the preventive control of the invasion of pathogenic microorganisms in daily life. Preventive disinfection refers to the disi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/54A61K9/00A61K47/69A61P31/02A61P17/00A61K38/47A61K38/48
CPCA61K38/47C12Y302/01017A61K38/4886C12Y304/24075C12Y302/01014A61K38/54A61K47/6951A61K9/0014A61K47/26A61P31/02A61P17/00A61K2300/00
Inventor 李慧许洋孙兴申潘仁杰
Owner 亘元(天津)生物医药科技有限公司
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