PTH rare earth detection kit, PTH rare earth detection card, microspheres of PTH rare earth detection card and preparation and detection methods

A detection card and rare earth technology, applied in biological testing, chemical instruments and methods, measuring devices, etc., can solve the problems of reducing detection sensitivity and interfering with detection signals, and achieve the effect of fast and simple detection, high signal-to-noise ratio, and low background

Active Publication Date: 2020-06-26
XIAMEN INST OF RARE EARTH MATERIALS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, many complexes and proteins in biological fluids and serum can fluoresce by themselves, and the spectral range of commonly used fluorescent markers is mostly in the visible light region

Method used

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  • PTH rare earth detection kit, PTH rare earth detection card, microspheres of PTH rare earth detection card and preparation and detection methods
  • PTH rare earth detection kit, PTH rare earth detection card, microspheres of PTH rare earth detection card and preparation and detection methods
  • PTH rare earth detection kit, PTH rare earth detection card, microspheres of PTH rare earth detection card and preparation and detection methods

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Rare earth nano fluorescent microspheres are core-shell neodymium-doped gadolinium lutetium fluoride-coated sodium yttrium fluoride, with a particle size of 40nm to 60nm, and its composition is:

[0052] NaGdxLu1-y-xF4:yNd@NaYF4, where NaGdxLu1-xF4 is the host and the dopant ion is Nd 3+ The colon ":" represents neodymium doping; x and y are rare earth ion doping mole percentages, and the range of x is 20% to 90%, preferably 40%; the range of y is 1% to 10%, preferably 5 %; NaYF4 is the shell layer, @ indicates that NaYF4 is coated on the surface of NaGdxLu1-y-xF4:yNd.

[0053] Further, the rare earth nano fluorescent microspheres are stable in the ground state, and emit fluorescence with a wavelength range of 1000-1100 nm under the action of an excitation light source of 800-1000 nm.

Embodiment 2

[0055] The method for preparing rare earth nano fluorescent microspheres comprises the following steps:

[0056] Step 1: Synthesis of neodymium-doped gadolinium lutetium fluoride: In a container, add oleic acid and 1-octadecene in a volume ratio of 3-6:7-14, and then add x parts of gadolinium nitrate or Acetate or chloride, y parts of nitrate or acetate or chloride of neodymium, 1-x-y parts of nitrate or acetate or chloride of lutetium, x and y are the molar percentages of rare earth ion doping, x The range of y is 20%~70%, the range of y is 0~30%; mix and stir at room temperature, vacuumize, then raise the temperature to 100~120°C, react for 20~30 minutes, then raise the temperature to 150~160°C, react 10-15 minutes to obtain a transparent solution; naturally cool to 40-50 ° C, release the vacuum, add a mixed solution of NaOH and ammonium fluoride methanol with a molar concentration ratio of 1-2:1.6-3.4, and react for 20-30 minutes; heat up to 90 ~100°C, pump air for 3~4 tim...

Embodiment 3

[0060] Such as figure 1 As shown, the PTH detection card includes:

[0061] Substrate 1; as the substrate of the PTH detection card;

[0062] Coating film 3; arranged in the middle of the upper surface of the substrate 1;

[0063] Sample pad 2; overlapped at one end of the upper surface of the coating film 3;

[0064] Absorbent paper 4; overlapped on the other end of the upper surface of the coating film 3;

[0065] Wherein, the end of the sample pad 2 close to the coating film 3 is sprayed with a microsphere line 21, and the microsphere line 21 is the PTH monoclonal antibody labeled with the rare earth nano fluorescent microspheres of embodiment 1; A detection line 31 and a quality control line 32 are provided, the detection line 31 is close to the sample pad 2, the detection line 31 and the quality control line 32 are parallel to each other, and the distance between them is 3-5mm; the detection line 31 is coated with PTH Monoclonal antibody, control line 32 coated with g...

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Abstract

The invention discloses a PTH rare earth detection kit, a PTH rare earth detection card, microspheres of the PTH rare earth detection card, and preparation and detection methods of the microspheres. Wherein the rare earth nano fluorescent microsphere is neodymium-doped gadolinium lutetium fluoride coated sodium yttrium fluoride with a core-shell structure, the particle size is 40-60 nm, the rare earth nano fluorescent microsphere is composed of NaGdxLu1-y-xF4: yNd-coated NaYF4, NaGdxLu1-xF4 is a matrix, and doped ions are Nd < 3 + >; wherein the number is shown as neodymium doping; wherein x and y are rare earth ion doping mole percentages, x ranges from 20% to 90%, and y ranges from 1% to 10%; wherein NaYF4 is a shell layer, and at the moment, NaYF4 represents that NaGdxLu1-y-xF4 is coated on the surface of NaGdxLu1-y-xF4: yNd. The fluorescent microsphere disclosed by the invention is a rare earth fluoride near-infrared two-area light-emitting nano material and has the advantages thatthe background is low; the microsphere is connected with the antibody through a covalent bond, so that the labeled product is stable, has the characteristics of wide detection range (10-1000pg/mL), high sensitivity (the detection limit is 10pg/mL), high accuracy, quickness, simplicity and convenience in detection and the like, and can be used for quick judgment in an operation.

Description

technical field [0001] The invention relates to a PTH detection kit, a detection card and microspheres thereof, and a preparation and detection method. Background technique [0002] Thyroid cancer is the fastest-rising solid malignancy worldwide. Currently, surgery is the preferred treatment for thyroid cancer, and parathyroid damage is the main surgical injury. Postoperative hypoparathyroidism caused by parathyroid injury remains a difficult problem for thyroid surgeons. [0003] Parathyroid glands are often not easily distinguished from fat droplets, lymph nodes, ectopic thyroid, and ectopic thymus. The parathyroid glands secrete parathyroid hormone (parathyroid hormone related peptide, referred to as PTH), and PTH is only specifically expressed in the parathyroid glands, and other tissues cannot express PTH. Improve the effectiveness and efficiency of doctors in quickly identifying parathyroid glands in clinic. [0004] The detection methods for measuring PTH in the p...

Claims

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Application Information

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IPC IPC(8): C09K11/02C09K11/85G01N33/558G01N33/577G01N33/58G01N33/78B82Y30/00
CPCC09K11/7773C09K11/02G01N33/78G01N33/582G01N33/587G01N33/577G01N33/558B82Y30/00
Inventor 张云张肖宋良
Owner XIAMEN INST OF RARE EARTH MATERIALS
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