A detection method based on resistance micropore particle counter and its application

Abstract

The invention discloses a detection method based on a resistance microporous particle counter and its application, and relates to the fields of food safety, clinical diagnosis and environmental monitoring. N parts of insulating microspheres with different particle sizes are in one-to-one correspondence with the analyte; according to the analyte, specific recognition molecules are modified on the surface of the corresponding insulating microsphere, and magnetically separated after interacting with the analyte. The number of reacting insulated microspheres is determined; analytes include biomarkers, drug residues, antibiotics and / or bacteria. The invention establishes the quantitative relationship between the object to be tested and the number of microspheres by means of immunoreaction, DNA molecular hybridization, etc., and then measures the number of insulating microspheres by means of a resistance micropore particle counter to indirectly obtain the concentration of the object to be tested. , the present invention can not only realize the simultaneous detection of multiple indicators, but also has low cost, good stability and high accuracy.

Description

technical field [0001] The invention relates to the fields of food safety, clinical diagnosis and environmental monitoring, in particular to a detection method based on a resistance micro-pore particle counter and its application. Background technique [0002] Food safety, environmental pollution and human health are the livelihood issues that have attracted much attention at present, and corresponding food testing, environmental monitoring and clinical diagnosis are the basis for improving the above problems. Currently, the most widely used detection methods in food testing, environmental testing and clinical diagnosis usually include instrumental analysis, immunoassay and molecular diagnostic techniques. [0003] The instrumental analysis method has the advantages of high sensitivity and good accuracy, but the sample pretreatment is complex, time-consuming, and the detection cost is high, and it is not suitable for on-site or clinical detection. [0004] Colloidal gold im...

AI Technical Summary

Problems solved by technology

[0003] Instrumental analysis has the advantages of high sensitivity and good accuracy, but the sample pretreatment is complicated, time-consuming, and the detection cost is high, and it is not suitable for on-site or clinical detection

[0004] Colloidal gold immunochromatography test strips, enzyme-linked immunoassay and chemiluminescence immunoassay are the most widely used immunoassay methods at present. Colloidal gold immunochromatography test strips are characterized by fast analysis speed and simple operation. , has been widely used in the fields of food safety rapid detection and clinical diagnosis, but the sensitivity of the test strip is low, and it is difficult to detect trace target substances; although the sensitivity of the enzyme-linked immunoassay is higher than that of colloidal gold The paper strip is high, but only one target can be detected at a time, and each detection requires cumbersome operations, and the detection speed is slow; chemiluminescence immunoassay has high sensitivity, but not only requires the enzyme Catalysis, and the operation steps are relatively cumbersome, and only one target can be detected at a time. When there are many targets, it will take a lot of time and cost

[0005] Molecular diagnostic technology has the advantages of high sensitivity and specificity, but it has strict requirements on the detection environment, is easily polluted by the external environment, and the cost of equipment is high, so it is difficult to be used for rapid on-site detection

[0006] In recent years, flow cytometry, liquid phase suspension chip technology and electrochemical detection methods based on nanopores have gradually emerged and received some attention. The characteristics of simultaneous detection have been widely concerned by researchers, but the equipment is expensive and the detection cost is high, which is not conducive to popularization and use; while the electrochemical detection method based on nanopores has high sensitivity, but in the detection, with the sensitivity The higher the improvement, the more external interference factors it receives, such as the interference of the matrix in complex samples, resulting in poor stability and difficult to use in the detection and analysis of complex samples

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