Preparation method and application of icariin sustained-release nano-gel by aerosol targeted drug delivery
A technology of icariin and nano-gel is applied in the field of medicine to achieve the effects of reducing asthmatic airway inflammatory response, good bioadhesion, and reducing the level of cytokines
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[0042] Example 1 Preparation of icariin-alginate nanogel
[0043] 1.1 Preparation of sodium alginate-water solution
[0044] Under the condition of 45℃ (usually 40-50℃), mix sodium alginate and water uniformly to make a sodium alginate-water solution with a mass-volume concentration of 0.50% (m / v, usually 0.25-0.75%) ;
[0045] 1.2 Preparation of zinc chloride-ethanol solution
[0046] Mix zinc chloride with an ethanol solution with a concentration of 60% (usually 50-70%) by volume percentage, and vortex to mix evenly to prepare chlorine with a concentration of 0.1% (m / v, usually 0.1-0.2%) Zinc-ethanol solution;
[0047] 1.3 Preparation of icariin-zinc chloride solution
[0048] Add icariin to the zinc chloride-ethanol solution, and vortex to mix. The ratio of the mass of icariin to the volume of the zinc chloride-ethanol solution is 0.3: 100 (usually (0.1- 0.4): 100), that is, 0.3 g (usually 0.1-0.4 g) of icariin is added to 100 mL of zinc chloride-ethanol solution to prepare an icar...
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[0055] Example 2 Characterization of Icariin-Zinc Alginate Nanogel
[0056] 2.1 Particle size and Zeta potential test
[0057] Dilute the prepared zinc alginate nanogel solution and icariin-zinc alginate nanogel solution 10 times, and measure the particle size, particle size distribution and surface zeta potential of the nanogel with a nanoparticle sizer, at room temperature The measurement was repeated 3 times, and the results were averaged. The test results are shown in Table 1.
[0058] Table 1 Particle size and Zeta-potential of zinc alginate nanogel (Means±SD)
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[0061] 2.2 Fourier transform infrared spectrum analysis
[0062] Dry to constant weight sodium alginate, icariin, icariin-zinc chloride-sodium alginate mixture, zinc alginate nanogel and icariin-zinc alginate nanogel with bromine respectively Using potassium bromide tablets as a control, measure the infrared spectrum of the sample on a Fourier transform infrared spectrometer, and set the wave number rang...
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[0064] Example 3 Drug loading rate and encapsulation rate of icariin-zinc alginate nanogel
[0065] Preparation of drug loading rate sample solution: accurately weigh 3 copies of icariin-zinc alginate nanogel, 25.00mg each, add appropriate amount of methanol to the sample and extract several times by ultrasonic, extract the icariin monomer to a constant volume In a 25 mL volumetric flask, after standing still, the supernatant was taken and filtered using a 0.45 μm pore size microporous filter membrane, and the content of icariin was determined by HPLC.
[0066] Preparation of encapsulation rate sample solution: accurately weigh 3 copies of the same batch of icariin-zinc alginate nanogel, 25.00mg each, add an appropriate amount of ultrapure water, sonicate for 30 minutes and then dilute it into a 25mL volumetric flask. After standing still, take the supernatant and add it to the ultrafiltration tube. After centrifugation, save the solution in the sleeve for use. The experimental re...
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