Marine streptomyces and application thereof
A technology of marine Streptomyces and bacterial strains, applied in the field of microorganisms, can solve problems such as harm and agricultural production loss, and achieve the effect of significant anti-downy mildew activity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0042] Example 1. Marine Streptomyces Streptomyces pratensis KCB-132 of acquisition.
[0043] The isolation medium was Gao’s No. 1 medium, 50 mg / L cycloheximide, 50 mg / L nystatin and 20 mg / L nalidixic acid were added as inhibitors, and the sterilization conditions were 121 ° C for 20 min. The actinomycetes in the marine sediment samples were separated by the dilution coating method, and the separation plate was cultured at 28°C for 21 days, and the Streptomyces was obtained after purification Streptomyces pratensis KCB-132 .
[0044] Morphological growth characteristics:
[0045] will strain Streptomyces pratensis KCB-132 Inoculate on solid ISP2 medium and culture for 7 days. The bacteria can produce abundant white aerial hyphae, and the hyphae in the base are pale yellow. The colonies are dry and irregular.
[0046] Inoculate the strain on ISP2 medium, culture at 4°C, 10°C, 15°C, 20°C, 28°C, 32°C, 37°C, 42°C, 45°C, observe once a week, end in four weeks, repeat twic...
Embodiment 2
[0050] The preparation of embodiment 2 Kiamycin E
[0051] 1) Fermentation culture
[0052] Streptomyces marinum Streptomyces pratensis KCB-132 Streak inoculation on ISP2 solid medium, culture at 28°C for 3-4 days, until white spores grow, inoculate 10% of sporophyte inoculum into ISP2 liquid medium, 28°C, 150-220 rpm / min , shaker culture 8-12 d to harvest the fermented product. Filter the fermented product to obtain the fermented liquid and mycelia, and the fermented liquid passes through a macroporous adsorption resin column (12nm, 50 mu m), eluted with 100% ethanol, concentrated under reduced pressure with a rotary evaporator (vacuum degree 100mbar, rotation speed 100rpm) to obtain the total extract.
[0053] The ISP2 liquid medium is 0.4% glucose, 0.4% yeast powder, and 1% malt extract powder. Add 2% agar to the solid medium on this basis.
[0054] 2) Separation and purification of crude extract
[0055] The total extract was passed through normal phase silica gel ...
Embodiment 3
[0056] Example 3. Confirmation of the structure of Kiamycin E
[0057] 1. Instruments and materials
[0058] Jasco P-1020 digital polarimeter, Agilent TOF / 6500 high-resolution mass spectrometer, Shimadzu UV-2401 visible-ultraviolet spectrophotometer, and Bruke Avance III 500 NMR spectrometer for NMR, which were prepared according to the steps in Example 1 above.
[0059] 2. Compound structure identification
[0060] Kiamycin E: white powder, easily soluble in dimethylsulfoxide, methanol, acetone, chloroform, slightly soluble in water, [α]25D+ 38.7(MeOH, c 0.2); UV (Acetonitrile) λmax (log ε) 203 (3.6) , 262 (2.8), 336 (2.5)nm; HRESIMS [M + H]+, m / z325.10740 (calcd for C19H17O5, 325.10760).
[0061] The NMR data of Kiamycin E are shown in Table 1, figure 1 It is Kiamycin E positive ion mass spectrum (HR-ESI-MS). figure 2 Is Kiamycin E 1 H NMR spectrum (solvent: DMSO- d 6 ). image 3 Is Kiamycin E 13 C NMR spectrum (solvent: DMSO- d 6 ). Figure 4 is Kiamycin E DEP...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More