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Method for separating coenzyme Q10 by using supercritical fluid chromatography system

A supercritical fluid and chromatographic system technology, applied in the field of material separation and purification, can solve the problems of low purity and yield of coenzyme Q10 crude extract, large solvent consumption, long time consumption, etc. Effect

Active Publication Date: 2020-08-04
KINGDOMWAY BIOTECH (JIANGSU) CO LTD +2
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  • Abstract
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Problems solved by technology

[0007] The purpose of the present invention is to overcome the problems of low yield, long time consumption and large solvent consumption when the crude extract of coenzyme Q10 containing various coenzyme Q homologues is separated and purified by techniques such as silica gel column chromatography and recrystallization in the prior art. When the crude extract of coenzyme Q10 is separated and purified by simulated moving bed chromatography, there are defects of low purity and yield, and a method for separating coenzyme Q10 by using a supercritical fluid chromatography system is provided, which can obtain The coenzyme Q10 product with high purity and yield makes up for the research gap in the prior art of using supercritical fluid chromatography to separate and purify coenzyme Q10

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  • Method for separating coenzyme Q10 by using supercritical fluid chromatography system

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specific Embodiment approach

[0039] According to a specific embodiment of the present invention, the method for utilizing a supercritical fluid chromatography system to separate coenzyme Q10 includes:

[0040] (1) dissolving the coenzyme Q10 crude extract in an organic solvent to prepare the coenzyme Q10 feed liquid to be separated; turn on the supercritical fluid chromatography system, and set the system parameters;

[0041] (2) After the system runs stably, the chromatographic column is fully balanced, and the baseline is stable, inject the coenzyme Q10 feed solution to be separated, collect the coenzyme Q10 components according to the ultraviolet peak signal of the peak, and repeat the sample injection several times according to this separation process ;

[0042] (3) Use a rotary evaporator to remove the organic solvent and entrainer from the coenzyme Q10 fraction collected in the above steps to obtain high-purity coenzyme Q10.

[0043] The present invention will be described in detail below by way of...

Embodiment 1

[0046] In this embodiment, the chromatographic column specification is 10×250 mm, the stationary phase is C18, and the particle size is 25 μm. The method for separating coenzyme Q10 by a supercritical fluid chromatography system includes the following steps:

[0047] (1) Dissolve the crude extract of coenzyme Q10 in methanol to make coenzyme Q10 feed solution, the concentration is 0.5mg·mL -1 , the purity is 45%; open the supercritical fluid chromatography system, set the column temperature of its chromatographic column to be 30 ℃, the column pressure to be 7MPa, and the column pressure is a constant pressure, the mobile phase flow rate is 6CV / min, and the mobile phase is composed of supercritical carbon dioxide and an entrainer, the entrainer is ethanol and the proportion of the entrainer in the mobile phase is 5% v / v, and the ultraviolet wavelength is 220nm;

[0048] (2) After the system runs stably, the chromatographic column is fully balanced, and the baseline is stable, i...

Embodiment 2

[0051] In this example, the size of the chromatographic column is 15×250 mm, the stationary phase is silica gel coated with tris(3,5-dimethylphenylcarbamoylated) amylose, and the particle size is 45 μm. The supercritical fluid chromatography system separates the coenzyme The method of Q10 includes the following steps:

[0052] (1) Dissolve the crude extract of coenzyme Q10 in ethanol to make coenzyme Q10 feed solution, the concentration is 5mg·mL -1 , the purity is 52%; open the supercritical fluid chromatography system, set the column temperature of its chromatographic column to be 35°C, the column pressure to be 8.5MPa, and the column pressure to be a constant pressure, the mobile phase flow rate to be 5CV / min, and the mobile phase to be composed of supercritical Composed of carbon dioxide and an entrainer, the entrainer is isopropanol and the proportion of the entrainer in the mobile phase is 10% v / v, and the ultraviolet wavelength is 245nm;

[0053] (2) After the system i...

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Abstract

The invention belongs to the field of substance separation and purification, and relates to a method for separating coenzyme Q10 by using a supercritical fluid chromatographic system. The method comprises the following steps: (1) starting the supercritical fluid chromatographic system, and setting system parameters; (2) after the system operates stably, the chromatographic column is fully balanced, and the baseline is stable, injecting coenzyme Q10 feed liquid, and collecting the coenzyme Q10 component according to an ultraviolet peak signal of an appearance peak; and (3) removing the solventin the coenzyme Q10 component to obtain high-purity coenzyme Q10. According to the method, the coenzyme Q10 crude extract is treated by adopting a supercritical fluid chromatography system separationmethod for the first time, the coenzyme Q10 can be well separated from homolog of the coenzyme Q10, the obtained coenzyme Q10 is relatively high in purity and yield, organic waste liquid discharge amount is small, pollution is small, consumed time is short and separation efficiency is high; and the method has the characteristics of economy, high efficiency, easiness in operation and environmentalprotection, is beneficial to large-scale industrial popularization and application, and has a wide application prospect in the aspects of quantitative analysis and preparation separation.

Description

technical field [0001] The invention belongs to the field of material separation and purification, and in particular relates to a method for separating coenzyme Q10 by using a supercritical fluid chromatography system. Background technique [0002] Coenzyme Q10 (Coenzyme Q10, abbreviated as CoQ10), also known as ubiquinone, is a vitamin-like substance widely present in animals, plants and microorganisms. Coenzyme Q10 is a cell metabolism activator and antioxidant synthesized spontaneously by organisms. It can act on certain enzymes to cause changes in their three-dimensional structures, thereby affecting their physiological activities. Past research and clinical trials have proved that coenzyme Q10 not only has the effect of increasing the body's immunity and preventing cardiovascular and cerebrovascular sclerosis, but also helps to improve hypertension, congestive heart failure, nervous system diseases and treat tumors. At present, coenzyme Q10, as a precious natural produ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/74
CPCG01N30/06G01N30/74Y02P20/54
Inventor 胡泽君王炳荣廖炜程窦婵玉何清飞
Owner KINGDOMWAY BIOTECH (JIANGSU) CO LTD
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