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Xinhui citrus fermentation liquor as well as preparation method and application thereof

A technology for fermentation broth and citrus fruit, applied in the field of Xinhui citrus fermentation broth and its preparation, can solve the problems of long fermentation time, difficulty in meeting the diverse needs of different groups of people, and a single type of fermented fruit and vegetable products, and achieves the effect of solving a large number of wastes

Active Publication Date: 2020-08-07
INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the domestic fruit and vegetable fermented beverage industry is still in its infancy. Lack of special strains, backward large-scale preparation technology of compound bacterial agents, single types of fermented fruit and vegetable products, and difficulty in meeting the diverse needs of different groups of people are still relatively prominent problems.
[0004] The fermentation of new citrus mostly adopts the traditional fermentation process, and the fermentation time is relatively long, but there are relatively few technologies for inoculation and fermentation using dominant strains, especially for specific fermentation strains such as total flavonoids and lactic acid.

Method used

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  • Xinhui citrus fermentation liquor as well as preparation method and application thereof
  • Xinhui citrus fermentation liquor as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1: Adopt unicellular Saccharomyces cerevisiae ZLG-6 and Lactobacillus plantarum Picp-2 to ferment new citrus fruit pulp to prepare new citrus citrus fermented liquid of the present invention

[0042] 1. Seed liquid culture

[0043] Configure MRS solid medium (peptone 10g, beef extract 10g, yeast extract 5g, glucose 20g, triammonium citrate 2g, sodium acetate 5g, MgSO 4 0.1g, MnSO 4 0.05g, K 2 HPO 4 2g, Tween 80 1mL, agar 13g, pH 6.2. 1L of distilled water was dissolved and packed, sterilized at 115°C for 30min), and Lactobacillus plantarum picp-2 was streaked on a solid plate for 1-2d at 37°C in the dark , Pick a single colony and culture it in 1mL MRS liquid medium to obtain primary seed liquid. Then, the Lactobacillus plantarum suspension was expanded and cultivated at a ratio of 1:20 to level 2 (37° C. dark cultivation for 16 h), which was the Lactobacillus plantarum seed solution.

[0044] Configure YPD solid medium (10g of yeast extract, 20g of pept...

Embodiment 2

[0047] Embodiment 2: Comparison of the total flavonoid content of different microbial bacteria fermenting new citrus

[0048] 1. Grouping

[0049] Original pulp group, Lactobacillus plantarum Picp-2 group, Lactobacillus plantarum Picp-2+Saccharomyces cerevisiae RV171 (highly active Saccharomyces cerevisiae for online shopping Angel wine) group, Lactobacillus plantarum Picp-2+Saccharomyces cerevisiae ZLG-6 group , Lactobacillus G4 (lactic acid bacteria screened together with Lactobacillus plantarum Picp-2 in the laboratory) Saccharomyces cerevisiae ZLG-6 group, probiotics (commercially available Lactobacillus plantarum + Lactobacillus acidophilus + Bifidobacterium longum + rhamnos Lactobacillus sugar + Lactobacillus paracasei) + Saccharomyces cerevisiae RV171;

[0050] 2. Except the original fruit pulp, all the other groups are fermented according to the method in Example 1 (the number of viable bacteria in each group is consistent when inoculated with similar bacteria), and t...

Embodiment 3

[0054] Embodiment 3: the total flavonoid content of compound microbial fermentation blueberry

[0055] According to the method of Example 2, the fermented raw material is changed to blueberry pulp, and then the total flavonoid content is counted, and the results are shown in Table 3 below;

[0056] table 3

[0057] Total flavonoid content (g / kg) Puree 3.056 Picp-2+RV171 3.653 Picp-2+ZLG-6 3.738

[0058] As can be seen from Table 2, with more blueberries containing total flavonoids as fermentation raw materials, using the unicellular Saccharomyces cerevisiae ZLG-6 of the present invention and Lactobacillus plantarum Picp-2 to ferment, the total flavonoid content of the obtained fermentation raw materials reaches 3.738g / kg, compared with the 3.056g / kg of blueberry pulp, it only increased by more than 20%, and it was basically equivalent to the combination of Picp-2+RV171, which indicated that S. Flavonoids have their suitable fermentation raw ...

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Abstract

The invention relates to the technical field of microbial fermentation, and discloses Xinhui citrus fermentation liquor as well as a preparation method and application thereof. The Xinhui citrus fermentation liquor is obtained by fermenting Xinhui citrus with lactobacillus plantarum Picp-2 with the preservation number of CCTCC No: M 20191045 and saccharomyces cerevisiae ZLG-6 with the preservationnumber of CCTCC No: M 20191046. According to the invention, specially screened saccharomyces cerevisiae and lactobacillus plantarum are selected as dominant strains for Xinhui citrus fermentation andare mainly used for fermentation preparation of total flavonoids and lactic acid. The prepared Xinhui citrus fermentation liquor has relatively high total flavone and lactic acid components and relatively low ethanol content; compared with other citrus pulp, the content of total flavonoids of the Xinhui citrus pulp can be increased by over 40%, the content of lactic acid can be increased by over1200%; the Xinhui citrus fermentation liquor can be used as an effective component of cosmetics or food, and the problem that a large amount of Xinhui citrus pulp is wasted can be effectively solved.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a new citrus citrus fermentation liquid and its preparation method and application. Background technique [0002] As a national geographical indication product, Xinhui tangerine peel has a history of more than 700 years. It is mainly produced in Xinhui area of ​​Jiangmen City. It is one of the top ten authentic medicinal materials in Guangdong Province and one of the first eight Lingnan traditional Chinese medicinal materials protected by legislation. 1. It has the effects of regulating qi and invigorating the spleen, harmonizing the stomach, relieving vomiting, drying dampness and resolving phlegm. With the promotion of tangerine peel culture and the continuous expansion and deepening of processed products, the planting area of ​​tangerines has expanded year by year, the output of citrus fruits has increased rapidly, and the waste generated by Xinhui citrus fruit ...

Claims

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Application Information

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IPC IPC(8): C12P1/02C12P1/04A23L33/00A23L29/00A61K8/9789A61Q19/00C12R1/865C12R1/25
CPCC12P1/02C12P1/04A23L33/00A23L29/065A61K8/9789A61Q19/00A61K2800/85A61K2800/10C12R2001/25C12N1/205C12R2001/865C12N1/185A23V2400/169
Inventor 周映君彭源德谢纯良龚文兵朱作华
Owner INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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