Xinhui citrus fermentation liquor as well as preparation method and application thereof
A technology for fermentation broth and citrus fruit, applied in the field of Xinhui citrus fermentation broth and its preparation, can solve the problems of long fermentation time, difficulty in meeting the diverse needs of different groups of people, and a single type of fermented fruit and vegetable products, and achieves the effect of solving a large number of wastes
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Embodiment 1
[0041] Embodiment 1: Adopt unicellular Saccharomyces cerevisiae ZLG-6 and Lactobacillus plantarum Picp-2 to ferment new citrus fruit pulp to prepare new citrus citrus fermented liquid of the present invention
[0042] 1. Seed liquid culture
[0043] Configure MRS solid medium (peptone 10g, beef extract 10g, yeast extract 5g, glucose 20g, triammonium citrate 2g, sodium acetate 5g, MgSO 4 0.1g, MnSO 4 0.05g, K 2 HPO 4 2g, Tween 80 1mL, agar 13g, pH 6.2. 1L of distilled water was dissolved and packed, sterilized at 115°C for 30min), and Lactobacillus plantarum picp-2 was streaked on a solid plate for 1-2d at 37°C in the dark , Pick a single colony and culture it in 1mL MRS liquid medium to obtain primary seed liquid. Then, the Lactobacillus plantarum suspension was expanded and cultivated at a ratio of 1:20 to level 2 (37° C. dark cultivation for 16 h), which was the Lactobacillus plantarum seed solution.
[0044] Configure YPD solid medium (10g of yeast extract, 20g of pept...
Embodiment 2
[0047] Embodiment 2: Comparison of the total flavonoid content of different microbial bacteria fermenting new citrus
[0048] 1. Grouping
[0049] Original pulp group, Lactobacillus plantarum Picp-2 group, Lactobacillus plantarum Picp-2+Saccharomyces cerevisiae RV171 (highly active Saccharomyces cerevisiae for online shopping Angel wine) group, Lactobacillus plantarum Picp-2+Saccharomyces cerevisiae ZLG-6 group , Lactobacillus G4 (lactic acid bacteria screened together with Lactobacillus plantarum Picp-2 in the laboratory) Saccharomyces cerevisiae ZLG-6 group, probiotics (commercially available Lactobacillus plantarum + Lactobacillus acidophilus + Bifidobacterium longum + rhamnos Lactobacillus sugar + Lactobacillus paracasei) + Saccharomyces cerevisiae RV171;
[0050] 2. Except the original fruit pulp, all the other groups are fermented according to the method in Example 1 (the number of viable bacteria in each group is consistent when inoculated with similar bacteria), and t...
Embodiment 3
[0054] Embodiment 3: the total flavonoid content of compound microbial fermentation blueberry
[0055] According to the method of Example 2, the fermented raw material is changed to blueberry pulp, and then the total flavonoid content is counted, and the results are shown in Table 3 below;
[0056] table 3
[0057] Total flavonoid content (g / kg) Puree 3.056 Picp-2+RV171 3.653 Picp-2+ZLG-6 3.738
[0058] As can be seen from Table 2, with more blueberries containing total flavonoids as fermentation raw materials, using the unicellular Saccharomyces cerevisiae ZLG-6 of the present invention and Lactobacillus plantarum Picp-2 to ferment, the total flavonoid content of the obtained fermentation raw materials reaches 3.738g / kg, compared with the 3.056g / kg of blueberry pulp, it only increased by more than 20%, and it was basically equivalent to the combination of Picp-2+RV171, which indicated that S. Flavonoids have their suitable fermentation raw ...
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