High performance liquid chromatography method for simultaneously determining cellulose, hemicellulose and lignin in tobaccos and tobacco products

Abstract

A high performance liquid chromatography method for simultaneous determining cellulose, hemicellulose and lignin in tobacco and tobacco products comprises the following steps: S1, crushing and uniformly mixing tobacco and tobacco products, sieving, adding into a G3 sand core funnel, pickling, and filtering to obtain a first product; S2, washing the first product, drying, hydrolyzing with a sulfuric acid solution, and filtering to obtain supernatant and filter residues; S3, washing the filter residues, drying and weighing to obtain first residues with the mass of m1; S4, weighing the first residues after being subjected to ashing treatment, and the mass of the obtained second residues being m2; analyzing the contents of glucose, xylose, arabinose, galactose and mannose in the supernatant byadopting high performance liquid chromatography to obtain the contents of cellulose and hemicellulose; and obtaining the content of lignin according to (m1-m2). The method is simple in pretreatment,accurate in result and short in analysis time, can be used for simultaneously detecting the contents of cellulose, hemicellulose and lignin in tobaccos and tobacco products, and has the advantages ofbatch, rapidness, accuracy and the like.

Description

technical field [0001] The invention belongs to the technical field of tobacco and chemistry, and in particular relates to a high performance liquid chromatography method for simultaneously measuring cellulose, hemicellulose and lignin in tobacco and tobacco products. Background technique [0002] Cellulose is a straight-chain polysaccharide composed of 1,000 to 10,000 D-glucopyranose groups connected by β-1,4 glycosidic bonds. Its basic unit is cellobiose group, and the chains are combined along the long axis to form microfibers. Beam, with a high degree of stability and resistance to chemical degradation. The composition of hemicellulose is relatively complex, including many high-molecular sugars such as pentosan and hexosan. Hemicellulose is also insoluble in water, but unlike cellulose, hemicellulose is soluble in dilute alkali and easily hydrolyzed by acid. nature. Lignin is not a sugar. It is mainly a phenolic three-dimensional network polymer composed of three types...

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Problems solved by technology

[0005] Zhang Huailing et al. used the sample to be extracted by benzene-alcohol Soxhlet for 48 hours, then extracted with hot water for 3-4 times, separated lipids, sugars, starch, protein, pectin and other substances, dried and weighed. During the process, the solvent needs to be replaced many times, and the suction filtration and cleaning are many times. The experimental operation is more cumbersome and the test cycle is longer; The content of cellulose (ADL) and crude cellulose

In this method, the sample does not need to go through the degreasing process, but washing and filtering are important pretreatment processes, there are many washing steps, and the types of reagents used in the test process are still as high as 10 or more; the document with the application number CN201410520615.7 uses acetic acid 1. The nitric acid mixture destroys the outer layer of cellulose, and then hydrolyzes the cellulose with sulfuric acid and potassium dichromate under heating. The excess potassium dichromate is titrated with sodium thiosulfate, and potassium iodide and starch solution are used as indicators. , calculate the amount of potassium dichromate, and then convert it into cellulose content. This method uses many kinds of reagents and the detection method is complicated, and it cannot detect the content of hemicellulose and lignin at the same time, which has limitations.

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