Lactobacillus johnsonii and application thereof

A technology of Lactobacillus johnsonii and bacteria, which is applied in the field of microbiology, can solve the problems of less Lactobacillus johnsonii and fewer products, and achieve the effects of inhibiting pathogenic bacteria and anti-oxidation, broad application prospects, and good colonization ability

Active Publication Date: 2020-08-14
SHENYANG BOYANG FEED
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The native bacteria isolated from the animals themselves can better adapt to the gastrointestinal tract environment of the same species of animals, so that they can better exert the

Method used

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  • Lactobacillus johnsonii and application thereof
  • Lactobacillus johnsonii and application thereof
  • Lactobacillus johnsonii and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 Isolation and identification of blue fox source Lactobacillus johnsonii

[0051] 1.1 Medium for separation: MRS medium, the composition of the medium is as follows:

[0052]

[0053]

[0054]pH 6.2-6.5, sterilized at 121°C for 30min.

[0055] 1.2 Separation method:

[0056] (1) Sample collection and microbial isolation: select healthy adult blue foxes from the fur animal breeding base of the Institute of Specialty Products, Chinese Academy of Agricultural Sciences, collect the contents of the hindgut, and quickly put them into a sterilized screw-top test tube with a liquid medium After being brought back to the laboratory, serial dilution was carried out immediately, with three plates per gradient, separated and cultured by pouring method, and cultured upside down at 37°C.

[0057] (2) Separation and purification: Take the above plate with various bacteria, select a single colony of lactic acid bacteria, and use continuous streaking method to carry out...

Embodiment 2

[0067] Embodiment 2 Physiological and biochemical characteristics

[0068] The resuscitated strains were cultured for 24 hours, and the physiological and biochemical characteristics of lactic acid bacteria were identified by referring to "Bergey's Bacteria Identification Manual", "Common Bacteria System Identification Manual" and "Lactic Acid Bacteria Classification Identification and Experimental Methods", gelatin test, V-P test, MR test , sugar fermentation test, etc. are identified by bacterial trace biochemical reaction identification tubes.

[0069] Some physiological and biochemical identification results of strain ZJBF004 are shown in Table 1. Lactobacillus johnsonii ZJBF004 can utilize monosaccharides such as glucose, fructose, and galactose, disaccharides such as cellobiose, maltose, sucrose, and lactose, and escin, and has protease and amylase activities. The choice of available carbon sources is more abundant.

[0070] Table 1 Physiological and biochemical charact...

Embodiment 3

[0074] Embodiment 3 different temperature growth test

[0075] Inoculate the fresh bacterial solution cultured for 24 hours in the MRS liquid culture medium with an inoculum amount of 2.0%, and culture in constant temperature anaerobic at 20°C, 37°C and 55°C, respectively. Measure the OD at 0, 1, 2, 3, 4, 5, 6, 8, 10, 12, 14, 24, 36, 48h 600 Value, 3 parallels for each group. After the measurement is completed, take 3 groups of parallel test results to calculate the average value, and draw a growth curve according to the calculation results. The results are as follows: image 3 shown. It can be seen from the figure that after Lactobacillus johnsonii ZJBF004 was inoculated in MRS liquid medium, the fastest growth rate was obtained at 37°C. After 0-2 hours of inoculation, the growth is slow, and it is in the growth stagnation zone; after 2 hours, the absorbance value of the bacterial solution increases rapidly, and enters the logarithmic growth phase; after 10 hours, the abso...

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Abstract

The invention relates to the technical field of microorganisms, and particularly provides lactobacillus johnsonii and an application thereof. The preservation number of the lactobacillus johnsonii provided by the invention is CCTCC NO: M 2019976. The application of the lactobacillus johnsonii in preparation of products for regulating intestinal flora and the application of the lactobacillus johnsonii in preparation of antioxidants also belong to the protection range of the application. The strain provided by the invention is an excellent strain with potential probiotic properties, can be usedas a probiotic for antibacterial drugs, food additives and animal feed additives, and has high application prospects.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a kind of Lactobacillus johnsonii and its application. Background technique [0002] Lactobacillus johnsonii (L.johnsonii) is classified in the genus Lactobacillus. Studies have shown that Lactobacillus johnsonii has good probiotic effects, such as promoting the growth of piglets and reducing diarrhea in piglets; preventing poultry necrotic enteritis, improving the growth performance of broilers, and reducing the colonization of pathogenic bacteria in chickens; Immune dysfunction caused by protein energy imbalance, increase the gene expression of antigen receptors in small intestinal epithelial cells in mice and prevent non-alcoholic fatty liver disease in obese mice; another study showed that Lactobacillus johnsonii can stimulate secretory immunoglobulin A ( sIgA) production and regulation of cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L33/135A23K10/18A61K35/747A61P31/04A61P1/00A61P31/10A61P39/06C12R1/225
CPCC12N1/20A23L33/135A23K10/18A61K35/747A61P31/04A61P1/00A61P31/10A61P39/06A23V2002/00C12R2001/225C12N1/205A23V2400/151A23V2200/30A23V2200/3204Y02A50/30
Inventor 刘晗璐蔡熙姮司华哲李光玉
Owner SHENYANG BOYANG FEED
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