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Lactobacillus johnsonii and its application

A technology of Lactobacillus johnsonii and Staphylococcus, applied in the field of microorganisms, can solve the problems of less products and less Lactobacillus canis, and achieve the effects of inhibiting pathogenic bacteria and anti-oxidation, broad application prospects, and good colonization ability

Active Publication Date: 2022-05-24
SHENYANG BOYANG FEED
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The native bacteria isolated from the animals themselves can better adapt to the gastrointestinal tract environment of the same species of animals, so that they can better exert their probiotic properties and achieve the maximum therapeutic and health effects, but the report of Lactobacillus johnsonii from canis Less, fewer corresponding products in the market

Method used

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  • Lactobacillus johnsonii and its application
  • Lactobacillus johnsonii and its application
  • Lactobacillus johnsonii and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 Isolation and identification of blue fox-derived Lactobacillus johnsonii

[0051] 1.1 Medium for separation: MRS medium, the medium composition is as follows:

[0052]

[0053]

[0054]pH 6.2-6.5, sterilized at 121°C for 30min.

[0055] 1.2 Separation method:

[0056] (1) Sample collection and microbial isolation: Select healthy adult blue foxes from the fur animal breeding base of the Institute of Specialty Products, Chinese Academy of Agricultural Sciences, take the contents of the hindgut, and quickly put them into a sterile screw-cap test tube containing a liquid medium , carry out gradient dilution immediately after bringing it back to the laboratory, three plates per gradient, separate and culture by pouring method, and invert culture at 37°C.

[0057] (2) Separation and purification: Take the above plate with various bacteria, select single colony of lactic acid bacteria, and carry out separation and purification by continuous streaking method. ...

Embodiment 2

[0067] Example 2 Physiological and biochemical properties

[0068] The recovered strains were cultured for 24 hours, and the physiological and biochemical characteristics of lactic acid bacteria were identified by referring to "Berger's Bacteria Identification Manual", "Common Bacterial System Identification Manual" and "Lactic Acid Bacteria Classification Identification and Experimental Methods", gelatin test, V-P test, MR test , sugar fermentation test, etc. are identified by bacterial trace biochemical reaction identification tube.

[0069] Some physiological and biochemical identification results of strain ZJBF004 are shown in Table 1. Lactobacillus johnsonii ZJBF004 can utilize monosaccharides such as glucose, fructose and galactose, disaccharides such as cellobiose, maltose, sucrose, lactose, and esculin, and has protease and amylase activities. The choice of available carbon sources is more abundant.

[0070] Table 1 Physiological and biochemical characteristics of La...

Embodiment 3

[0074] Example 3 Growth test at different temperatures

[0075] The fresh bacterial broth cultured for 24 hours was inoculated into MRS liquid medium with 2.0% inoculum, and incubated anaerobic at constant temperature at 20°C, 37°C and 55°C, respectively. OD was measured at 0, 1, 2, 3, 4, 5, 6, 8, 10, 12, 14, 24, 36, 48h, respectively 600 value, each set of 3 parallels. After the measurement is completed, three groups of parallel test results are taken to calculate the average value, and the growth curve diagram is drawn according to the calculation results. The results are as follows image 3 shown. It can be seen from the figure that after Lactobacillus johnsonii ZJBF004 was inoculated in MRS liquid medium, the fastest growth rate was obtained at 37°C. 0-2h after inoculation, the growth is slow, and it is in the growth retardation zone; after 2h, the absorbance value of the bacterial solution rises rapidly and enters the logarithmic growth phase; after 10h, the absorbance...

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Abstract

The invention relates to the technical field of microorganisms, in particular, it provides Lactobacillus johnsonii and its application. The preservation number of Lactobacillus johnsonii provided by the present invention is CCTCC NO: M 2019976. The application of Lactobacillus johnsoni in the preparation of products for regulating intestinal flora and the application of antioxidants also belongs to the protection scope of the present application. The bacterial strain provided by the invention is an excellent bacterial strain with potential probiotic properties, can be used as probiotics in antibacterial drugs, food additives and animal feed additives, and has very broad application prospects.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a Lactobacillus johnsonii and applications thereof. Background technique [0002] Lactobacillus johnsonii (L. johnsonii) is classified in the genus Lactobacillus. Studies have shown that Lactobacillus johnsonii has good probiotic effects, such as promoting the growth of piglets and reducing piglet diarrhea; preventing and treating necrotizing enteritis in poultry, improving the growth performance of broilers, and reducing the colonization of pathogenic bacteria in chickens; Immune dysfunction caused by protein-energy imbalance, increase the gene expression of antigen receptors in small intestinal epithelial cells in mice, and prevent non-alcoholic fatty liver disease in obese mice; another study showed that Lactobacillus johnsonii can stimulate secretory immunoglobulin A ( sIgA) production and regulation of cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α), interl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23L33/135A23K10/18A61K35/747A61P31/04A61P1/00A61P31/10A61P39/06C12R1/225
CPCC12N1/20A23L33/135A23K10/18A61K35/747A61P31/04A61P1/00A61P31/10A61P39/06A23V2002/00C12R2001/225C12N1/205A23V2400/151A23V2200/30A23V2200/3204Y02A50/30
Inventor 刘晗璐蔡熙姮司华哲李光玉
Owner SHENYANG BOYANG FEED
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