Culture method of trichloromethane degrading bacteria
A technology of chloroform and cultivation method, which is applied in the direction of using microorganisms, chemical instruments and methods, biochemical equipment and methods, etc. It can solve the problems such as the difficulty of cultivating chloroform-degrading bacteria, shorten the cultivation period and suppress volatilization , to ensure full mixing effect
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Embodiment 1
[0041] The present embodiment provides a kind of culture method of chloroform degrading bacteria, described method comprises the steps:
[0042] Adding waste water containing chloroform and nutrients required for microbial growth into the closed reactor, the concentration of chloroform in the waste water is 20-100 mg / L;
[0043] Inoculate activated sludge into the reactor, adjust the pH of the reaction solution to 6.0-9.0, pass oxygen into the liquid surface, make the pressure above the liquid surface in the reactor be 0-0.1MPa, stir, and react until culturing Finish.
[0044] Control the height of the vortex generated by the liquid level below 0.5cm during stirring, so that the wastewater in the reactor can fully contact with the oxygen above the liquid level, and at the same time avoid the volatilization of trichloromethane in the wastewater caused by violent stirring.
[0045] During the reaction, an alkaline solution is used to absorb carbon dioxide gas generated during t...
Embodiment 2
[0050] The present embodiment provides a kind of culture method of chloroform degrading bacteria, described method comprises the steps:
[0051] Adding wastewater containing chloroform and nutrients required for microbial growth into the reactor, the concentration of chloroform in the wastewater is 20-100mg / L;
[0052] Inoculate activated sludge into the reactor, adjust the pH of the reaction solution to 6.0-9.0, pass oxygen into the liquid surface, make the pressure above the liquid surface in the reactor be 0-0.1MPa, stir, and react until culturing Finish.
[0053] Control the height of the vortex generated by the liquid level below 0.5cm during stirring, so that the wastewater in the reactor can fully contact with the oxygen above the liquid level, and at the same time avoid the volatilization of trichloromethane in the wastewater caused by violent stirring.
[0054] During the reaction, an alkaline solution is used to absorb carbon dioxide gas generated during the reactio...
Embodiment 3
[0062] The present embodiment provides a kind of culture method of chloroform degrading bacteria, described method comprises the steps:
[0063] see figure 1 , set up a 11cm*11cm*11cm square plexiglass airtight reactor 1 with an effective volume of 1.2L, add 1.0L chloroform wastewater into the reactor, the concentration of chloroform in the wastewater is 40mg / L, and add 10g of water with a water content of 99 % of sewage plant aeration tank sludge, add 30mg calcium chloride, add 0.1mg ferric chloride, add 10mg magnesium sulfate, 5mg ammonium chloride, 300mg dipotassium hydrogen phosphate, adjust the pH of the solution in the reactor to be 7.5, pass Oxygen channel 4 feeds oxygen in the reactor 1, and the pressure on the liquid surface in the control reactor by the pressure gauge 2 is 0.05Mpa, and in the open reservoir 5, add the sodium hydroxide solution of 3mL 15% (mass concentration), The carbon dioxide gas produced during the cultivation of the degrading bacteria reacts wit...
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