A kind of recombinant escherichia coli producing mannan and its application

A technology for recombining Escherichia coli and mannan, which is applied in the field of bioengineering, can solve the problems of low yield, complex extraction process, and high molecular weight of konjac mannan, and achieves a technology with less extracellular secretion impurities, strong growth efficiency, and easy separation and purification. Effect
CN111607548BActive Publication Date: 2021-11-16ZHEJIANG FORESTRY UNIVERSITY
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Patent Information

Authority / Receiving Office
CN ยท China
Patent Type
Patents(China)
Current Assignee / Owner
ZHEJIANG FORESTRY UNIVERSITY
Publication Date
2021-11-16

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Abstract

The invention discloses a mannan-producing recombinant escherichia coli and an application thereof, belonging to the technical field of bioengineering. The present invention adopts the mannan synthase gene ManS derived from the plant guar, integrates it into the pET21a plasmid controlled by the T7 promoter, and realizes the synthesis of mannan in the recombinant plasmid in Escherichia coli E.coli BL21 (DE3). Heterogeneous synthesis, the yield can reach 0.68g / L. The invention lays a certain foundation for the high-efficiency fermentation and preparation of mannan by microorganisms using cheap carbon sources, and is suitable for industrial production and application.
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Description

technical field

[0001] The invention relates to a mannan-producing recombinant Escherichia coli and an application thereof, belonging to the technical field of bioengineering. Background technique

[0002] Mannan is a hemicellulose polysaccharide widely present in various life forms. As an excellent low-calorie, high-cellulose water-soluble dietary fiber, mannan is widely used in food and medicine industries. Mannan has unique biological activity due to its unique molecular structure and physical and chemical properties, and can participate in some very important physiological processes in the body, such as stimulating the body's immune response, promoting the vegetative growth of beneficial bacteria in the intestine, and reducing serum cholesterol levels Wait.

[0003] Mannan is widely found in plants such as konjac flour, guar gum, field gum and dendrobium, as well as in the cell walls of various microorganisms. The current commercialized mannan is mainly extracted from ...

Claims

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