Anaerobic immobilized bacterial preparation, as well as preparation method and application thereof

Abstract

The invention belongs to the field of environmental engineering materials and particularly relates to an anaerobic immobilized bacterial preparation, as well as a preparation method and application thereof. The preparation method comprises the following steps: preparing corresponding culture bacterial solutions from four different anaerobic functional strains by utilizing a pure bacterial culturetechnology; mixing the four culture bacterial solutions in a certain volume ratio to obtain a compound functional bacterial solution; concentrating the compound functional bacterial solution into functional flora precipitation, and dissolving the functional flora precipitation in an polyvinyl alcohol aqueous solution; dropping the solution into a first buffer solution to obtain polyvinyl alcohol gel beads; and putting the gel beads into a second buffer solution containing sulfate to obtain sulfate modified polyvinyl alcohol gel beads, namely the anaerobic immobilized bacterial preparation. Thepreparation method is simple, has low energy consumption; and the anaerobic immobilized bacterial preparation is acid-resistant and ammonia-resistant, can remarkably enhance the treatment performanceof flora inside an anaerobic digestion reactor on accumulated organic acid and solves the problems of functional strain loss and poor self-healing performance of anaerobic flora.

Description

technical field [0001] The invention belongs to the field of environmental engineering materials, and in particular relates to an anaerobic immobilized bacterial agent, a preparation method and an application thereof. Background technique [0002] As one of the effective biological treatment technologies, anaerobic digestion can not only control the environmental pollution of easily degradable biomass waste, but also convert the organic matter contained in it into methane-based energy gas. When the feed is biomass waste with high solid content (such as kitchen waste, poultry manure, sewage sludge, etc.), organic acids mainly acetic acid are easy to accumulate rapidly in the anaerobic digestion reactor, causing Acid inhibition of methanogens can lead to instability in the methanation process and even failure of the reactor. Especially when ammonia inhibition occurs at the same time, ammonia inhibition will further aggravate the problem of organic acid accumulation, forming d...

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Problems solved by technology

At present, there are four main problems in bioaugmentation measures: 1. Suspension culture mode is easy to cause the loss of additional functional flora; 2. It is difficult for the additional flora to adapt to the physical and chemical conditions inside the reactor, and it will die quickly, or its competitive advantage will be lost by the reactor. The internal indigenous microorganisms surpass; 3. The structure of the external bacterial group is not specific, a single strain is easy to die, and the compound strain lacks synergistic metabolism, resulting in low efficiency, and it is necessary to increase the inoculation amount and inoculation frequency; 4. Difficulty in cultivating anaerobic pure strains Large, long generation time, unable to meet the large-scale needs of large-scale reactors; 5. The external bacterial colony is not tolerant to high ammonia concentrations, and cannot solve the problem of organic acid accumulation under ammonia-inhibited conditions

Publication number is that the Chinese invention patent of CN103014070B has prepared the compound enzyme agent based on liquefying enzyme, glucoamylase, cellulase and lipase, and the Chinese invention patent of publication number is CN106085926A has prepared Pelotomaculum shinkii (Shi's dark color anaerobic sausage Such technologies promote the metabolism of microorganisms, thereby alleviating the inhibition of organic acids, but none of these technologies have achieved the immobilization of enzymes and anaerobic agents, resulting in the loss of enzymes and agents , to weaken the effect of

However, the method of using immobilized bacterial agents to release the inhibition of anaerobic digestion organic acids in situ has not been reported.

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