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Injectable hydrogel system of combining chemotherapy with photothermal therapy and preparation thereof

A hydrogel and injectable technology, applied in the field of nano-biomedicine, can solve the problems of the biosafety and degradability of hydrogels, the limited effect of tumor treatment, and the inability to directly apply tumor treatment. Chemotherapy, rich drug loading, slow release effect

Inactive Publication Date: 2020-10-02
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the biosafety and degradability of these hydrogels are of concern
In addition, many chemotherapeutic drugs cannot be directly applied to tumor treatment because they are insoluble in water.
[0003] In addition, the therapeutic effect of a single treatment method on tumors is very limited,

Method used

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  • Injectable hydrogel system of combining chemotherapy with photothermal therapy and preparation thereof
  • Injectable hydrogel system of combining chemotherapy with photothermal therapy and preparation thereof
  • Injectable hydrogel system of combining chemotherapy with photothermal therapy and preparation thereof

Examples

Experimental program
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Effect test

example 1

[0039] Example 1: PC 10 Preparation of A / PTX / HAuNS / DOX Injectable Hydrogel

[0040] Hollow nano-gold shells were prepared by "cobalt template sacrificial method": 0.1mM CoCl 2 ·6H 2 O and 0.1mM C 6 h 5 N 3 o 7 2H 2 O was dissolved in 100 mL of water, and the mixture was stirred for 60 min to generate cobalt nanoparticles, and finally 430 μL of HAuCl was added dropwise to the mixture4 · 3H2O. Continue to stir the mixture for 25 minutes to form a gold shell, and finally use the oxygen in the air to oxidize cobalt to cobalt ions to form a hollow nano-gold shell. Centrifuge at 12,000 rpm for 15-25 minutes, suspend in ultrapure water, and repeat the centrifugation three times to obtain purified hollow gold nanoparticles. shell.

[0041] Mix 0.5 ml of 8 mg / mL hydrophilic chemotherapeutic drug doxorubicin (DOX) aqueous solution with purified 0.5 ml of 80 ug / mL hollow gold nano shell solution and stir overnight. 12000rpm centrifugation removes excess chemotherapeutic drugs, a...

example 2

[0044] Example 2: PC 10 Sustained Drug Release of A / PTX / HAuNS / DOX Injectable Hydrogel

[0045] 100 microliters of PC prepared in Example 1 10 A / PTX / HAuNS / DOX injectable hydrogel was horizontally centrifuged and transferred to the cap of a 1.5 ml PE tube, then loaded with PC 10 The lid of the A / PTX / HAuNS / DOX injectable hydrogel was placed upside down in pH 5.0 acetate buffer to simulate drug release in an acidic tumor environment. The drug release behavior of PTX and DOX was observed by ultraviolet absorption spectroscopy and high performance liquid chromatography. Such as figure 2 Experimental results show that after a week of drug release, the cumulative release amounts of the two drugs reach 60-85% respectively, indicating that the injectable hydrogel has a good drug sustained-release effect.

example 3

[0046] Example 3: PC 10 Photothermal effects of A / PTX / HAuNS / DOX injectable hydrogel

[0047] PC 10 A / PTX / HAuNS / DOX injectable hydrogel at different power (0.5w / cm 2 ,1.0w / cm 2 ,1.5 w / cm 2 ,2.0w / cm 2 ,2.5w / cm 2 ) Changes in temperature within 9 minutes under 808nm laser irradiation. Experimental results such as image 3 As shown, as the laser power increases and the illumination time prolongs, the PC 10 The A / PTX / HAuNS / DOX injectable hydrogel was gradually raised under different power laser irradiation, and the temperature exceeded 42 degrees, which was enough to kill tumor cells.

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Abstract

The invention discloses an injectable hydrogel system of combining a chemotherapy with a photothermal therapy and preparation thereof. The injectable hydrogel system is an injectable hydrogel and comprises a PC10A nano hydrogel loaded with a hydrophobic drug and a nanogold shell system loaded with a hydrophilic drug, wherein the hydrophobic drug is supported in a hydrophobic cavity of the PC10A nano hydrogel; the hydrophilic drug is loaded in a nanogold shell; and the PC10A nano hydrogel is formed by denaturation, cooling and self-assembly of a polypeptide PC10A. The injectable hydrogel disclosed by the invention can load the hydrophilic drug and the hydrophobic drug at the same time, has high drug loading capacity and long drug release time, can achieve slow release of the hydrophilic drug and the hydrophobic drug, has a good photothermal effect, can be successfully and noninvasively applied to long-term treatment of tumors by combining the chemotherapy with the photothermal therapy in situ through an injector, and can significantly improve the treatment effect.

Description

technical field [0001] The invention belongs to the technical field of nano-biomedicine, and specifically relates to the preparation of an injectable hydrogel that can be loaded with various drugs with different solubility properties, realize slow release of drugs, and combine chemotherapy and photothermal therapy. Background technique [0002] Conventional chemotherapeutic drugs are directly used for tumor treatment through intravenous injection, which tends to cause serious side effects on normal tissues. After the drug enters the blood circulation, it is easily eliminated by metabolism, and the injectable hydrogel-coated nanoparticle drug-loading system can realize in-situ drug delivery. Common hydrogels include chemically synthesized hydrogels and natural macromolecular hydrogels, such as UV-induced synthetic hydrogels and polyacrylamide hydrogels. However, the biosafety and degradability of these hydrogels are concerns. In addition, many chemotherapeutic drugs cannot ...

Claims

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Application Information

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IPC IPC(8): A61K9/06A61K47/42A61K47/02A61K41/00A61K31/704A61K31/337A61P35/00
CPCA61K9/0019A61K9/06A61K31/337A61K31/704A61K41/0052A61K47/02A61K47/42A61P35/00A61K2300/00
Inventor 刘波金瑞美杨洁赵元弟
Owner HUAZHONG UNIV OF SCI & TECH
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