Micro-ecological composite additive containing saccharomyces cerevisiae and application of micro-ecological composite additive

A compound additive and Saccharomyces cerevisiae technology, which is applied to Saccharomyces cerevisiae and its application in aquaculture, can solve problems such as damage to normal flora structure, harm to human health, and drug resistance of pathogenic bacteria, so as to promote the growth of farmed animals and promote Effect of animal growth and improvement of meat quality

Active Publication Date: 2020-10-02
青岛玛斯特生物技术有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, excessive use of antibiotics will not only destroy the normal flora structure of the prawn intestinal tract, but also easily lead to drug resistance of pathogenic bacteria.

Method used

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  • Micro-ecological composite additive containing saccharomyces cerevisiae and application of micro-ecological composite additive
  • Micro-ecological composite additive containing saccharomyces cerevisiae and application of micro-ecological composite additive
  • Micro-ecological composite additive containing saccharomyces cerevisiae and application of micro-ecological composite additive

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: Isolation, screening and identification of strains

[0029] 1. Sample:

[0030] It was collected from a Litopenaeus vannamei farm in Rizhao City. Healthy and vigorous prawn intestines were picked by aseptic operation for subsequent separation.

[0031] 2. Screening method

[0032] Take the intestinal sample and put it into a grinder, put it into a normal saline, homogenize it, and centrifuge at a low speed, so that the bacteria in the sample are fully dispersed in the upper layer of normal saline, dilute it in a 10-fold gradient, and inoculate it on 2216E, TSB and MRS solid medium respectively. Cultivate at 28°C for 24h-48h. Select a uniform and clear single colony and streak to purify the bacteria. The single colonies were named MST01, MST02, MST03..., MST30 accordingly.

[0033] Using Vibrio parahaemolyticus and Vibrio harveyi as indicator bacteria, probiotics were screened by plate antagonism method. Spot the bacterial liquid to be screened on a pl...

Embodiment 2

[0042] Example 2 Effects of Saccharomyces cerevisiae MST01 on growth, immunity and stress resistance of Litopenaeus vannamei

[0043] In this experiment, a control group and a probiotic group were set up, and each group was set up in three parallels, with 200 Litopenaeus vannamei in each parallel. The high-temperature-cured commercial compound feed is used as the basic feed. Among them, the control group was fed with basal feed, and the probiotic group was fed with 10 7 CFU / g feed for S. cerevisiae MST01. The breeding experiment lasted for 56 days. The prawns were fed at 3-5% of their body weight every day, and the amount of bait was adjusted at any time according to the food intake. They were fed once a day in the morning and evening, and sucked and discharged once a day. During the experiment, the dissolved oxygen was ≥7mg / l, the temperature was 29±1°C, the salinity was 21-22‰, and the pH was 8.0±0.3. After the breeding experiment, the Litopenaeus vannamei were counted an...

Embodiment 3

[0052] Example 3 Effects of Saccharomyces cerevisiae MST01 on muscle quality and fatty acid composition of Litopenaeus vannamei

[0053] Saccharomyces cerevisiae MST01 was added to the diet of Litopenaeus vannamei, and the texture analyzer and gas chromatography were used to analyze the changes of muscle texture and fatty acid content of the shrimp after feeding, so as to study the effect of Saccharomyces cerevisiae MST01 on the muscle quality of Litopenaeus vannamei.

[0054] 1. Preparation of bacterial suspension

[0055] Saccharomyces cerevisiae MST01 was inoculated in the nutrient medium with an inoculum of 1%, cultured statically at 37°C for 20 hours, centrifuged at 4000r / min for 15 minutes after cultivation, washed 3 times with sterile normal saline, and resuspended in sterile normal saline , adjust the bacterial suspension concentration to 10 9 CFU / mL.

[0056] 2. Preparation of bacteria-containing feed

[0057] Add the treated bacterial suspension to the commercial ...

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Abstract

The invention relates to the technical fields of screening and application of functional microorganisms, and specifically provides a micro-ecological composite additive and application thereof in aquaculture. The micro-ecological composite additive contains Saccharomyces cerevisiae MST01 and has a preservation number of CCTCC NO:M2020092. According to the micro-ecological composite additive, the freed utilization rate of bred animals can be remarkably increased, growth of the bred animals is promoted, the meat quality is improved, the immunity of aquatic animals can be further enhanced, the full-cycle survival rate is improved, and the micro-ecological composite additive can be widely applied to the field of aquatic feeds.

Description

technical field [0001] The invention relates to the technical field of functional microorganism screening, in particular to a strain of Saccharomyces cerevisiae and its application in aquaculture. Background technique [0002] With the increase in demand for aquatic products and the international development of aquatic product trade, the aquaculture industry has developed by leaps and bounds in recent years. However, the intensification and high-density scale of aquaculture is expanding day by day, the deterioration of the aquaculture environment and the harm of diseases have brought huge economic losses to the aquaculture industry. [0003] Litopenaeus vannamei occupies a large proportion in my country's aquaculture industry due to its adaptability to a wide range of salinity. With the rapid development of Litopenaeus vannamei aquaculture, the diseases of Litopenaeus vannamei are becoming more and more serious, and antibiotics have become the traditional preferred solution...

Claims

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Application Information

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IPC IPC(8): C12N1/18A23K50/80A23K10/18A23K20/163A23K20/174C12R1/865
CPCA23K10/18A23K20/163A23K20/174A23K50/80C12N1/18
Inventor 王磊周怡刘广郭本月魏万权倪梦丽
Owner 青岛玛斯特生物技术有限公司
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