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Method for preparing solid-phase carbon quantum dots by adopting biological collagen waste and application of solid-phase carbon quantum dots

A technology of carbon quantum dots and biological glue, which is applied in the field of nanomaterial preparation, can solve the problems of difficult purification of liquid phase products, complicated preparation process, and difficult transportation, and achieve good fluorescence properties and modifiability, wide sources, and high safety Effect

Inactive Publication Date: 2020-10-13
ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these methods can prepare fluorescent carbon quantum dots, a series of reaction conditions such as complex preparation process, long time consumption, low yield, and expensive precursors often limit the possibility of large-scale preparation of carbon quantum dots. properties, thus limiting the practical application prospects of carbon quantum dots
Although there are some reports on methods for preparing carbon quantum dots in large quantities, the use of a large number of oxidizing reagents and expensive and complex precursors makes the preparation method not environmentally friendly; Many defects such as low cost and difficult transportation

Method used

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  • Method for preparing solid-phase carbon quantum dots by adopting biological collagen waste and application of solid-phase carbon quantum dots
  • Method for preparing solid-phase carbon quantum dots by adopting biological collagen waste and application of solid-phase carbon quantum dots
  • Method for preparing solid-phase carbon quantum dots by adopting biological collagen waste and application of solid-phase carbon quantum dots

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Experimental program
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Effect test

Embodiment 1

[0042] (1) Carbon source preparation: add 100 g of bovine tendon waste remaining after collagen extraction in the factory to 5 L of 0.5 M acetic acid solution, add 10 g of pepsin, and stir at 4°C for 2 days. Filter the collagen waste after soaking with gauze, and wash thoroughly with deionized water. After cleaning, freeze overnight in a -20°C freezer, then freeze-dry in a freeze dryer for 36 hours, and dry at an oven temperature of 40°C for 8 hours, and finally obtain a white gauze wing-shaped solid that is the carbon source precursor;

[0043] (2) Carbonized carbon source: Weigh 20 g of the carbon source obtained in step (1) and place it in a porcelain boat, and place it in a tube furnace. Under the protection of nitrogen, the temperature is raised from room temperature to 300 ℃ and keep it warm for 6 hours; when the temperature drops to room temperature, take out the porcelain boat to obtain the brown-yellow carbon quantum dot product;

[0044] (3) Purification: Dissolve all...

Embodiment 2

[0046] (1) Preparation of carbon source: Add 100g of rat tail waste left after collagen extraction in the factory to 5L of 0.1M hydrochloric acid-nitric acid mixture (volume ratio 1:1), and add 10g of pepsin, and stir at 4°C 3d. Filter the collagen waste after soaking with gauze, and wash thoroughly with deionized water. After cleaning, freeze overnight in a freezer at -20°C, then freeze-dry in a freeze dryer for 36 hours, and dry at an oven temperature of 50°C for 6 hours, and finally obtain a white gauze wing-shaped solid that is the carbon source precursor;

[0047] (2) Carbonized carbon source: Weigh 20 g of the carbon source obtained in step (1) and place it in a porcelain boat, and place it in a tube furnace. Under the protection of argon, the temperature is raised from room temperature to 400°C and keep it warm for 4 hours; when the temperature drops to room temperature, take out the porcelain boat to get the brown-yellow carbon quantum dot product;

[0048] (3) Purif...

Embodiment 3

[0050] (1) Preparation of carbon source: add 5L of 0.3M nitric acid-silicotungstic acid mixture (volume ratio 3:1) to 100g of mixed waste of bovine tendon, rat tail, and fish skin after extracting collagen from the factory, and add 10g of pepsin, stirred at 4°C for 5d. Remove the remaining large pieces of tissue and then filter with gauze to obtain the enzymatic hydrolysis and acidification product, which is fully washed with deionized water. After cleaning, freeze overnight in a -20°C freezer, then freeze-dry for 48 hours in a lyophilizer, and dry at an oven temperature of 60°C for 4 hours, and finally obtain a white gauze wing-like solid that is the carbon source precursor;

[0051] (2) Carbonized carbon source: Weigh 20g of the carbon source obtained in step (1) and place it in a porcelain boat, and place it in a tube furnace, and raise the temperature to 600°C at a rate of 10°C / min under the protection of argon And keep it warm for 2 hours; when the temperature drops to r...

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Abstract

The invention relates to a method for preparing solid-phase carbon quantum dots by adopting biological collagen waste and application of the solid-phase carbon quantum dots. The preparation method ofthe carbon quantum dots comprises the steps of (1) collecting the biological collagen waste generated by a factory as a carbon source; (2) further carrying out enzymolysis and acidification treatmenton the obtained biological collagen waste, freeze-drying and calcining to obtain a solid-phase carbon quantum dot crude product; and (3) collecting and purifying the crude product powder to obtain thecarbon quantum dot material. The preparation method is simple and easy to operate; raw materials are wide in source and easy to obtain; the reutilization of the biological collagen waste is realized;the method has the advantages of being green and free of pollution, biological tissue waste is recycled with higher value, the yield of the prepared carbon quantum dots is high, complete conversion from a carbon source to carbon quantum dot products is basically achieved, the method is particularly suitable for large-scale preparation of the carbon quantum dots, and the prepared carbon quantum dots can be used as in-vivo and in-vitro imaging agents and have the advantages of being high in safety and low in toxic and side effects.

Description

technical field [0001] The invention relates to a method and application for preparing solid-phase carbon quantum dots by using biological collagen waste, and belongs to the technical field of nanomaterial preparation. Background technique [0002] Fluorescent carbon quantum dots with a size below 10nm have received great attention due to their low photobleaching, good biocompatibility, good water solubility, and excellent cell membrane permeability. These properties make carbon quantum dots superior to organic dyes and inorganic heavy metal semiconductor quantum dots in biological systems. [0003] The preparation of carbon quantum dots is mainly divided into "top-down" method and "bottom-up" method. The "top-down" method refers to cutting or decomposing carbon quantum dots from bulk graphite structures by chemical oxidation, hydrothermal, solvothermal, electrochemical exfoliation, laser ablation, etc. The "bottom-up" method is obtained by polymerization carbonization of ...

Claims

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Application Information

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IPC IPC(8): C01B32/15C09K11/65B82Y20/00B82Y40/00C12Q1/02G01N21/64
CPCC01B32/15C09K11/65C09K11/0883B82Y20/00B82Y40/00G01N33/5005G01N21/6428G01N2021/6432
Inventor 秦肖雲赵建波陈凤华秦笑梅
Owner ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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