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Method for detecting hydrogen peroxide and related targets based on CaS nano fluorescent probe

A nanoprobe and detection method technology, applied in the field of detecting hydrogen peroxide and related targets based on CaS nanofluorescent probes, can solve demanding problems

Pending Publication Date: 2020-10-27
FUJIAN INST OF RES ON THE STRUCTURE OF MATTER CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the main methods for the detection of hydrogen peroxide and related substances include electrochemical methods, chromatography and spectrophotometer methods, etc., but these methods have certain deficiencies. For example, these methods have relatively high requirements for instruments and samples. , and cannot avoid the interference of complex components in blood, making these methods unable to achieve rapid and accurate direct detection of hydrogen peroxide and related substances

Method used

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  • Method for detecting hydrogen peroxide and related targets based on CaS nano fluorescent probe
  • Method for detecting hydrogen peroxide and related targets based on CaS nano fluorescent probe
  • Method for detecting hydrogen peroxide and related targets based on CaS nano fluorescent probe

Examples

Experimental program
Comparison scheme
Effect test

preparation example 1

[0086] Preparation example 1 oil-soluble CaS:0.07mol%Ce 3+ ,0.5mol%Er 3+ Preparation of nanoparticles

[0087] 1) Weigh 0.9943mmol Ca(CH 3 COO) 2 ·H 2 O, 0.0007mmol Ce(CH 3 COO) 3 4H 2 O, 0.005 mmol Er(CH 3 COO) 3 4H 2 O Then add 2mL oleic acid, 6mL oleylamine and 12mL trioctylamine, heat to 120°C with nitrogen gas and keep it warm for 30 minutes to form a transparent solution A;

[0088] 2) Then cool down to room temperature; weigh 3mmol of DPTU (N,N'-diphenylthiourea), dissolve it in 10mL of ethanol, and add it dropwise to solution A, continue to stir for 10min to fully mix, and heat up Heat at 80°C for 30 minutes to form a transparent solution B;

[0089] 3) Raise the temperature to 320°C, keep it warm for 1 hour, and then lower it to room temperature; add 20mL of ethanol to precipitate, separate and wash several times to obtain oil-soluble CaS of about 26nm: 0.07mol%Ce 3+ ,0.5mol%Er 3+ nanoparticles.

[0090] figure 2 In a is oil-soluble CaS:0.07mol%Ce 3+ ...

preparation example 2

[0091] Preparation example 2 water-soluble CaS:0.07mol%Ce 3+ ,0.5mol%Er 3+ Preparation of nanoparticles

[0092] 1) the oil-soluble CaS obtained in Preparation Example 1:0.07mol%Ce 3+ ,0.5mol%Er 3+ Nanoparticles were dissolved in chloroform to obtain solution one, CaS in solution one: 0.07mol% Ce 3+ ,0.5mol%Er 3+ The concentration of nanoparticles is 2 mg / mL;

[0093] 2) DSPE-PEG-NH dissolved in chloroform 2 Add it dropwise into solution 1 under constant stirring to obtain solution 2;

[0094] Step (1) oil-soluble CaS:0.07mol%Ce 3+ ,0.5mol%Er 3+ Nanoparticles with DSPE-PEG-NH 2 The mass ratio is 1:5;

[0095] 3) React solution 2 at 30°C for about 5 hours, and wait for the chloroform to evaporate completely;

[0096] 4) Centrifuge after step 3) and wash with deionized water several times to obtain DSPE-PEG-NH 2 The modified water-soluble rare earth-doped CaS nanomaterials are redispersed in ultrapure water and stored at 4°C for use;

[0097] image 3 For CaS:0.07m...

Embodiment 1

[0100] The present embodiment is used for the biological detection of hydrogen peroxide, and the following are specific operation steps:

[0101] (1) Use a polystyrene 96-well plate as the carrier used for detection, and add 8 rows of 100 μL of CaS:0.07mol% Ce obtained in Preparation Example 2 to the set microwells 3+ ,0.5mol%Er 3+ Aqueous solution of nanoparticles, CaS:0.07mol% Ce 3+ ,0.5mol%Er 3+ The concentration of the aqueous solution of nanoparticles is 200, 100, 50, 25, 10, 5, 2.5, 1 μg / mL in turn, and 100 μL of hydrogen peroxide with a concentration of 20 μM is added in turn to obtain 8 groups of mixed solutions; Shake for 2 hours, respectively measure the luminous intensity of the 8 groups of mixed solutions, and the luminous quenching efficiency of the mixed solutions of each group; when the luminous quenching efficiency is the largest, the corresponding CaS of the mixed solution: 0.07mol% Ce 3+ ,0.5mol%Er 3+ The nanoparticle concentration was 0.5 mg / mL.

[0102...

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Abstract

The invention belongs to the technical field of biological detection, and discloses a method for detecting hydrogen peroxide and related targets based on a nanoprobe. According to the method, a water-soluble rare earth doped CaS: Ce<3+> / Ln<3+> nano material is used as a fluorescent probe, rare earth ion luminescence is quenched through an oxidation-reduction reaction of hydrogen peroxide and cerium ions, and the concentration of hydrogen peroxide is detected by utilizing the change of the fluorescence intensity of doped rare earth ions. The method can be used for detecting hydrogen peroxide ina standard solution or reactants in an enzymatic reaction for generating hydrogen peroxide, can further realize the detection of hydrogen peroxide, biological enzyme or substrate (such as xanthine) in the serum, has the advantages of being simple and convenient to operate, good in anti-interference performance, rapid, sensitive, economical, practical and the like, can provide theoretical basis and technical support for real-time detection of hydrogen peroxide and hydrogen peroxide generation system related substances in a complex system, and has certain clinical application potential.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a method for detecting hydrogen peroxide and related targets based on a CaS nanometer fluorescent probe. Background technique [0002] Hydrogen peroxide (H 2 o 2 , commonly known as hydrogen peroxide) solution is a colorless and odorless liquid, and is currently a strong oxidizing agent widely used in the paper and textile industries. Many biochemical reactions in the human body will produce hydrogen peroxide, such as xanthine will produce hydrogen peroxide under the catalysis of xanthine oxidase. However, excessive accumulation of hydrogen peroxide will accelerate the aging of the human body, induce cancer, and cause diseases such as diabetes and kidneys. At present, more and more people suffer from hyperuricemia, gout and other diseases due to bad living habits and eating habits, and patients suffer from diseases. Therefore, realizing accurate and r...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/643G01N2021/6432
Inventor 郑伟张美然陈学元刘龑委娇娇宫仲亮
Owner FUJIAN INST OF RES ON THE STRUCTURE OF MATTER CHINESE ACAD OF SCI
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