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PH response slow release selenium-containing protein composite nanoparticles and preparation and application

A composite nanoparticle and selenoprotein technology, which is applied in the field of selenoprotein-containing composite nanoparticles and the preparation thereof, can solve the problems of less comprehensive utilization, and achieve the effects of improving the degree of deep processing and utilization, reducing the cost of products, and improving the insoluble properties of drugs

Active Publication Date: 2020-11-06
DALIAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using chlorogenic acid-grafted macromolecules to improve solubility, antioxidant activity, and inductive directivity, etc., so that it can be used to improve the tolerance of drug carriers in the gastrointestinal tract, and achieve a certain degree of non-specific targeted release and absorption sustained release function, but the comprehensive utilization of the combination of the two is relatively rare

Method used

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  • PH response slow release selenium-containing protein composite nanoparticles and preparation and application
  • PH response slow release selenium-containing protein composite nanoparticles and preparation and application
  • PH response slow release selenium-containing protein composite nanoparticles and preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The preparation of a pH-responsive slow-release selenoprotein composite nanoparticle comprises the following steps:

[0047] A. Pre-treat fresh selenium-enriched black soybeans and extract 11S selenium-enriched black soybean protein:

[0048] Soak fresh selenium-enriched black soybeans, pulverize them at a low temperature of 4°C, mix the powder with water, defat, and air-dry to obtain selenium-enriched black soybean defatted powder; at a solid-liquid ratio of 1:15, use 0.01 of pH 6.4 under the mechanism of alkali extraction and acid precipitation 11S protein was extracted with mol / L sodium sulfite, desalted by dialysis, and freeze-dried to obtain selenium-enriched black soybean 11S protein powder.

[0049] B. Chlorogenic acid radical grafting chitosan modification:

[0050] Dissolve chitosan in 2% acetic acid solution at a ratio of 1:20 to prepare 5% chitosan solution; mix chitosan:hydrogen peroxide:ascorbic acid mass ratio of 250:17:27, stir for 30min, and follow The...

Embodiment 2

[0059] The preparation of a pH-responsive slow-release selenoprotein composite nanoparticle comprises the following steps:

[0060] A, the extraction of selenium-enriched black soybean 11S protein powder is the same as in Example 1.

[0061] B, the preparation of chlorogenic acid grafted chitosan is the same as that in Example 1.

[0062] C. Compounding the selenium-enriched black soybean 11S protein of step A with the chlorogenic acid-grafted chitosan of step B to prepare a nanocomposite carrier: 6 mg / mL of selenium-enriched black soybean 11S protein solution and the modified polysaccharide in step B by mass The ratio of 3:1-1:3 is mixed (protein: polysaccharide), the pH is 3.5, and the composite nanoparticles are prepared by physical mixing. The particle size and potential of composite nanoparticles prepared with different proteoglycan ratios are shown in Table 2.

[0063] D. Dissolve curcumin in ethanol and mix with the carrier of step C, stir in the dark, disperse the so...

Embodiment 3

[0069] The preparation of a pH-responsive slow-release selenoprotein composite nanoparticle comprises the following steps:

[0070] A, the extraction of selenium-enriched black soybean 11S protein powder is the same as in Example 1.

[0071] B, the preparation of chlorogenic acid grafted chitosan is the same as that in Example 1.

[0072] C. Compounding the selenium-enriched black soybean 11S protein in step A with the chlorogenic acid-grafted chitosan in step B to prepare a nanocomposite carrier: 6 mg / mL selenium-enriched black soybean 11S protein solution and the modified polysaccharide or shell in step B Polysaccharides were mixed at a mass ratio of 3:1 (protein: polysaccharides), pH was 3.5, and composite nanoparticles were prepared by physical mixing or saccharification reaction and heating at 90°C for 30 minutes. The particle size and potential of composite nanoparticles prepared by different polysaccharides and composite methods are shown in Table 3.

[0073] D. Disso...

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Abstract

The invention belongs to the technical field of a nano-carrier material, and particularly relates to pH response slow release selenium-containing protein composite nanoparticles and preparation and application thereof. The pH response slow release selenium-containing protein composite nanoparticles disclosed by the invention specifically is of a core-shell structure, wherein a selenium-containingblack bean 11S protein is used as a core, and chlorogenic acid grafted chitosan is used as a shell. According to the invention, the cores of the nano-carrier particles are constructed by using a new resource selenium-containing black bean 11S protein component as a base material, a non-specific targeted chlorogenic acid active ingredient is adopted to be grafted with a food byproduct chitosan, andthe chlorogenic acid grafted chitosan is used as an outer layer to improve solubility, environment resistance and the induction targeting ability of the composite nanoparticles, so that compatibilityand non-specific specificityof the nanoparticles are obviously improved; and the specific self-assembly property of the selenium-containing 11S protein component enables a hydrophilic and lyophobic functional composite carrier to be formed in the compounding process; and when the pH response slow release selenium-containing protein composite nanoparticles are applied to the fields of the food industry and drug loading, a loading amount reaches over 19%, and encapsulation efficiency is over 95%.

Description

technical field [0001] The invention belongs to the technical field of nano-carrier materials, in particular to a pH-responsive slow-release selenoprotein-containing composite nanoparticle, a preparation method and application thereof. Background technique [0002] Dosage forms provide key technologies for the dissolution of various drugs and the formation of different crystal forms of drugs. The drug dosage form of the response slow control system can be designed according to the specific environmental conditions or the internal environment of special parts of the human body, which solves the problem of hydrophobic drugs such as curcumin. Due to problems such as easy oxidation, large crystal form and insoluble water, fast metabolism, and low bioavailability, it provides the possibility for effective relief of specific symptoms. The selection of carrier materials follows the principle of non-toxic and harmless to the human body, but most of the carrier materials on the marke...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K9/52A61K47/36A61K47/42A61K31/12A61K31/704A61K31/216A61K33/04A61P39/06A23L33/185A23L33/125A23L29/10A23P10/30
CPCA61K9/5161A61K9/5169A61K31/12A61K31/704A61K31/216A61K33/04A61P39/06A23L33/185A23L33/125A23L29/10A23P10/30A23V2002/00A61K2300/00A23V2200/222A23V2250/1626A23V2250/511A23V2250/548
Inventor 朱雪梅牟光庆陈桢隆熊华
Owner DALIAN POLYTECHNIC UNIVERSITY
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