Bacillus amyloliquefaciens KY09 and application thereof in deodorization

A technology of amylolytic spores and bacilli, applied in the field of microorganisms, can solve problems such as high cost, large energy consumption, and environmental pollution, and achieve the effects of reducing odor pollution, increasing economic value, and reducing energy consumption

Active Publication Date: 2020-11-06
JINHUA KOYON ENVIRONMENTAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] If the ammonia and hydrogen sulfide produced by the aerobic fermentation of kitchen waste are not treated, it will not only pollute the environment, but also seriously affect the physical and mental health of the surrounding residents.
Therefore, how to remove ammonia and hydrogen sulfide efficiently and at low cost has become one of the technical problems in the resource utilization of aerobic fermentation of kitchen waste. Although some commercially available biomass waste treatment equipment has deodorization systems, there are still Problems such as poor deodorization effect, large energy consumption, and high cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The cow dung samples in the cow dung heap were obtained from Jinhua Jiufeng Ranch, and then the samples were inoculated into the screening medium. The preparation of the screening medium was as follows: 5.0 g of beef extract, 10.0 g of protein, 5.0 g of sodium thiosulfate, chlorine Ammonium chloride 5.0g, water 1000ml, pH value 7.0; prepare 100ml screening medium, add appropriate amount of sterile glass beads, insert 1.0g cow dung sample, and then carry out enrichment for 7 days.

[0034] The enriched culture solution was inserted into the separation medium by the gradient dilution method. The preparation of the separation medium was as follows: 10.0 g of glucose, 6.0 g of ammonium chloride, 5.0 g of sodium thiosulfate, 4.0 g of dipotassium hydrogen phosphate, Potassium dihydrogen phosphate 2.0g, magnesium chloride 0.2g, trace elements 2ml (zinc chloride 4.0g / L, calcium chloride 5.0g / L, manganese chloride 5.0g / L, ferrous chloride 5.0g / L, molybdic acid Ammonium 1.0g / L, c...

Embodiment 2

[0036] The bacterial classification that embodiment 1 obtains carries out the preliminary test of ammonia nitrogen and hydrogen sulfide degradation, specifically as follows:

[0037] Preparation of liquid medium: glucose 10.0g, ammonium chloride 6.0g, sodium thiosulfate 5.0g, dipotassium hydrogen phosphate 4.0g, potassium dihydrogen phosphate 2.0g, magnesium chloride 0.2g, trace elements 2ml (zinc chloride 4.0g / L, calcium chloride 5.0g / L, manganese chloride 5.0g / L, ferrous chloride 5.0g / L, ammonium molybdate 1.0g / L, copper chloride 2.0g / L, cobalt chloride 1.0g / L , adjust the pH to 7.0), water 1000ml. In a 500ml Erlenmeyer flask, add 100ml of liquid medium, and sterilize it at 121°C in an autoclave for 20 minutes; establish 3 parallel experimental groups and 3 control groups, wherein 3 experimental groups are connected to Example 1 The obtained bacterial strain (the inoculum amount is 1% of the culture medium) was not added any bacterial species to the 3 control groups.

[0...

Embodiment 3

[0041] This example provides a method for understanding the fermentation of Bacillus amylobacter KY09.

[0042] After the strain activation of Bacillus amyloliquefaciens KY09, inoculate in fermentation medium (beef extract 5.0g, egg white 10.0g, sodium thiosulfate 5.0g, ammonium chloride 6.0g, manganese sulfate 0.05g, water 1000ml, pH value 7.0), cultured at 37° C. and 200 rpm for 24 hours to prepare the fermentation broth of Bacillus amyloliquefaciens KY09.

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PUM

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Abstract

The invention relates to bacillus amyloliquefaciens KY09 and application thereof in deodorization. The classification name of the bacterial strain is Bacillus amyloliquefaciens KY09, the bacterial strain is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO:M 2020153. The strain can remove ammonia nitrogen and hydrogen sulfide at the same time, can effectively degrade odor generated by kitchen garbage fermentation, reduces odor pollution, and has the advantages of high efficiency and no need of additional energy consumption. When the strain is used for treating the odor of the kitchen garbage, the energy consumption in the resource utilization process of the kitchen garbage can be remarkably reduced, energy conservation and emission reductionare achieved, and more economic values are brought to resource utilization of the kitchen garbage.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a strain of bacillus amyloliquefaciens KY09 and its application in deodorization. Background technique [0002] Aerobic fermentation resource utilization of kitchen waste is one of the new treatment methods for kitchen waste disposal. However, a large amount of ammonia and hydrogen sulfide will be produced during the aerobic fermentation process. Both ammonia and hydrogen sulfide have pungent odor and corrosion. Sex, not only harms human health, but also pollutes the environment. Ammonia and hydrogen sulfide are the main components of the fermentation odor of kitchen waste. [0003] Ammonia gas is very soluble in water, and reacts with acid to produce ammonium salt, which mainly exists in two forms of ammonium sulfate and ammonium nitrate. Ammonium nitrogen is one of the main forms of ammonia gas, and it is easy to change under alkaline conditions. Ammonia gas escapes fr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A62D3/02B01D53/84B01D53/52B01D53/58C12R1/07
CPCC12N1/20A62D3/02B01D53/84B01D53/52B01D53/58B01D2251/95C12R2001/07C12N1/205Y02A50/20
Inventor 张康东潘盛淇李卓蕾
Owner JINHUA KOYON ENVIRONMENTAL TECH CO LTD
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