Meyerozyma guilliermondii and application thereof in biological deodorization
A biological deodorization and yeast technology, applied in the biological field, can solve the problems of secondary pollution, high equipment cost and high operation requirements, and achieve the effect of no biological hazard, high deodorization efficiency and obvious degradation effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0031] Example 1 Screening and identification of Pichia pastoris
[0032] 1. Pick out bones, napkins and other substances from the kitchen waste in Yuxiu Canteen of Zhejiang University of Technology, and then use a crusher to smash the remaining waste.
[0033] 2. Take 1g of chopped kitchen waste and dilute it to 1×10 with ultrapure water. -6 , 1×10 -7 , 1×10 -8 , and take 200 μL of each dilution to solid YPD medium, spread evenly with a coating rod, and place it in a constant temperature incubator at 28°C for 48h to 72h, pick a single colony for streak purification and culture;
[0034] The formula of YPD medium is: yeast powder 10g, peptone 20g, glucose 20g, agar 15g.
[0035] 3. After purifying each single colony, select the strains with pure white color, smooth and shiny shape, no wrinkle, and slight wine smell, and pick the single colony to extract the genome with the FastDNA Spin Kit for Soil kit.
[0036] 4. Use the extracted genome as a template for PCR. The PCR sy...
Embodiment 2
[0038] The preparation of embodiment 2 biological deodorant bacteria
[0039] 1. The Pichia moniliformis strain screened in Example 1 was inserted into the YPD medium.
[0040] 2. Inoculate the activated strains into YPD liquid medium for shaking culture (37°C, 180r / min) for more than 12 hours.
[0041] 3. Count the cultured bacterial liquid, and adjust the bacterial content to 10 8 ~10 9 CFU / ml.
[0042] 4. Centrifuge the bacterial liquid (12000 r / min, 10 min), discard the supernatant after centrifugation, and collect the bacterial cells.
[0043] 5. Mix the bacterial cells with diatomite and corn stalks at a ratio of 1:1 (w / w), and then place them in a 28°C incubator to dry and constant weight, that is, various bacterial agents. Diatomite is a molecular-grade product purchased from the market; corn stalks are pre-treated and then mixed with bacterial agents. The pre-treatment steps are: firstly crush the raw materials of corn stalks into 80-100 mesh powder, wash and dry ...
Embodiment 3
[0048] Example 3 Carry out airtight deodorization test with kitchen waste as substrate
[0049] The fresh kitchen waste was crushed with a blender and divided into 500ml Erlenmeyer flasks. Each Erlenmeyer flask was divided into 100g (wet weight) of fresh food waste. Three parallel experiments were set up for each group of experiments. Then the bacterial agent prepared in Example 2 was added in an amount of 10g / bottle, and a blank control was set simultaneously. Then seal it with three layers of plastic wrap, and tie the mouth of the bottle tightly with a rubber band. Place in an incubator at 28°C, and measure the concentrations of ammonia and hydrogen sulfide after 24 hours.
[0050] After three groups of parallel experiments, it was found that the degradation rate of hydrogen sulfide in kitchen waste was 79.23%, and the degradation rate of ammonia gas was 25.71% after inoculation of biological deodorizing bacteria agent for 24 hours. From the above experimental results, it ...
PUM
Login to view more Abstract
Description
Claims
Application Information
Login to view more - R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap