High performance liquid chromatography analysis method for determining vitamin A in complex component
A high-performance liquid chromatography, vitamin technology, applied in the direction of analysis of materials, measuring devices, material separation, etc., can solve the problems of complex and cumbersome operation process and many reagents, and achieve good repeatability, high accuracy and good accuracy.
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Embodiment 1
[0030] A1111-A2131-A3333 brand multivitamin and mineral tablets (containing vitamin A, vitamin E, vitamin B) produced by Shenzhen Flat Nutrition and Health Co., Ltd. 1 , Vitamin B 2 , niacin, pantothenic acid, vitamin B 6 , biotin, vitamin C, vitamin D, folic acid, vitamin B 12 , iron, zinc, selenium, magnesium, copper, calcium, chromium, potassium and other 20 components) determination of vitamin A content in samples. The specific detection steps of the first embodiment are as follows:
[0031] Step 1: Extraction solvent 0.025% ammonia solution Configuration: Measure 1.0ml of concentrated ammonia water, add it to 1000ml of ultrapure water, and shake well.
[0032] Step 2: Preparation of reference solution: Accurately weigh 42.0mg of vitamin A acetate reference substance, put it in a 50ml volumetric flask, add an appropriate amount of absolute ethanol to ultrasonically dissolve it, dilute it to the mark with absolute ethanol, shake well, and use it as a reference Product s...
Embodiment 2
[0046] Determination of vitamin A content in vitamin A tablet samples produced by Shenzhen Flat Nutrition and Health Co., Ltd. Specific steps:
[0047] Step 1: Preparation of 0.025% ammonia solution as extraction solvent: Measure 1.0ml of concentrated ammonia water, add it to 1000ml of ultrapure water, and shake well.
[0048] Step 2: Preparation of reference substance solution: Accurately weigh 4.0mg of vitamin A acetate reference substance, put it in a 50ml volumetric flask, add 95% ethanol to sonicate it to dissolve, dilute to the mark with 95% ethanol, shake well, and use it as a control Product stock solution Ⅰ; accurately measure 1ml of reference product stock solution Ⅰ, put it in a 100ml volumetric flask, dilute to the mark with 95% ethanol, shake well, and obtain.
[0049] Step 3: Preparation of sample solution: just before using the new preparation and avoiding light, take 20 pieces of this product, accurately weigh, grind into powder, accurately weigh and put an ap...
Embodiment 3
[0062] The multivitamin capsules (containing vitamin A, vitamin E, and vitamin B) produced by Shenzhen Flat Nutrition and Health Co., Ltd. 1 , Vitamin B 2 , niacin, pantothenic acid, vitamin B 6 , biotin, vitamin C, vitamin D, folic acid, vitamin B 12 , iron, zinc, selenium, magnesium, copper, calcium, chromium, potassium and other 20 components) determination of vitamin A content in samples. Specific steps are as follows:
[0063] Step 1: Preparation of 0.025% ammonia solution as extraction solvent: Measure 1.0ml of concentrated ammonia water, add it to 1000ml of ultrapure water, and shake well.
[0064] Step 2: Preparation of reference solution: Accurately weigh 42.0mg of vitamin A acetate reference substance, put it in a 50ml volumetric flask, add an appropriate amount of absolute ethanol to ultrasonically dissolve it, dilute it to the mark with absolute ethanol, shake well, and use it as a reference Product stock solution Ⅰ; accurately measure 1ml of the reference prod...
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