High performance liquid chromatography analysis method for determining vitamin A in complex component

A high-performance liquid chromatography, vitamin technology, applied in the direction of analysis of materials, measuring devices, material separation, etc., can solve the problems of complex and cumbersome operation process and many reagents, and achieve good repeatability, high accuracy and good accuracy.

Pending Publication Date: 2021-01-05
SHENZHEN AUSA PHARM CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the pretreatments used in the detection of vitamin A at this stage are high-temperature saponification, multiple extractions with n-hexane, sodium hydroxide and other reagents, nitrogen blowing, and re-dissolution. Many reagents are needed, and the operation process is complicated and cumbersome.

Method used

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  • High performance liquid chromatography analysis method for determining vitamin A in complex component
  • High performance liquid chromatography analysis method for determining vitamin A in complex component
  • High performance liquid chromatography analysis method for determining vitamin A in complex component

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A1111-A2131-A3333 brand multivitamin and mineral tablets (containing vitamin A, vitamin E, vitamin B) produced by Shenzhen Flat Nutrition and Health Co., Ltd. 1 , Vitamin B 2 , niacin, pantothenic acid, vitamin B 6 , biotin, vitamin C, vitamin D, folic acid, vitamin B 12 , iron, zinc, selenium, magnesium, copper, calcium, chromium, potassium and other 20 components) determination of vitamin A content in samples. The specific detection steps of the first embodiment are as follows:

[0031] Step 1: Extraction solvent 0.025% ammonia solution Configuration: Measure 1.0ml of concentrated ammonia water, add it to 1000ml of ultrapure water, and shake well.

[0032] Step 2: Preparation of reference solution: Accurately weigh 42.0mg of vitamin A acetate reference substance, put it in a 50ml volumetric flask, add an appropriate amount of absolute ethanol to ultrasonically dissolve it, dilute it to the mark with absolute ethanol, shake well, and use it as a reference Product s...

Embodiment 2

[0046] Determination of vitamin A content in vitamin A tablet samples produced by Shenzhen Flat Nutrition and Health Co., Ltd. Specific steps:

[0047] Step 1: Preparation of 0.025% ammonia solution as extraction solvent: Measure 1.0ml of concentrated ammonia water, add it to 1000ml of ultrapure water, and shake well.

[0048] Step 2: Preparation of reference substance solution: Accurately weigh 4.0mg of vitamin A acetate reference substance, put it in a 50ml volumetric flask, add 95% ethanol to sonicate it to dissolve, dilute to the mark with 95% ethanol, shake well, and use it as a control Product stock solution Ⅰ; accurately measure 1ml of reference product stock solution Ⅰ, put it in a 100ml volumetric flask, dilute to the mark with 95% ethanol, shake well, and obtain.

[0049] Step 3: Preparation of sample solution: just before using the new preparation and avoiding light, take 20 pieces of this product, accurately weigh, grind into powder, accurately weigh and put an ap...

Embodiment 3

[0062] The multivitamin capsules (containing vitamin A, vitamin E, and vitamin B) produced by Shenzhen Flat Nutrition and Health Co., Ltd. 1 , Vitamin B 2 , niacin, pantothenic acid, vitamin B 6 , biotin, vitamin C, vitamin D, folic acid, vitamin B 12 , iron, zinc, selenium, magnesium, copper, calcium, chromium, potassium and other 20 components) determination of vitamin A content in samples. Specific steps are as follows:

[0063] Step 1: Preparation of 0.025% ammonia solution as extraction solvent: Measure 1.0ml of concentrated ammonia water, add it to 1000ml of ultrapure water, and shake well.

[0064] Step 2: Preparation of reference solution: Accurately weigh 42.0mg of vitamin A acetate reference substance, put it in a 50ml volumetric flask, add an appropriate amount of absolute ethanol to ultrasonically dissolve it, dilute it to the mark with absolute ethanol, shake well, and use it as a reference Product stock solution Ⅰ; accurately measure 1ml of the reference prod...

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Abstract

The invention provides a liquid chromatographic analysis method for efficiently determining vitamin A in complex components. The method takes methanol water as a mobile phase for isocratic elution, can avoid the interference of other components, and can accurately and sensitively detect the content of vitamin A within 6 minutes. The method has the advantages of strong specificity, high precision,good stability, high accuracy, simple operation, and cheap and easily available reagents and consumables. The method is suitable for detecting the content of vitamin A in complex components of tablets, powder, capsules and other dosage forms containing vitamin A, the detection quality is controlled, and the detection cost and time can be effectively saved.

Description

technical field [0001] The invention relates to a liquid chromatography analysis method for efficiently and rapidly determining vitamin A content in complex components. Specifically, through the optimization of sample pretreatment and chromatographic conditions, the experiment is simple, convenient, accurate and fast to detect the content of vitamin A in complex components. Background technique [0002] Vitamin A (vitaminA), also known as retinol (its aldehyde derivative retinal) or anti-dry eye factor, is an unsaturated monohydric alcohol with an alicyclic ring, which plays an important role in the human body. For example, it can not only maintain normal visual function, maintain the health of epithelial tissue cells, promote the synthesis of immunoglobulin, maintain normal bone growth and development, promote growth and reproduction, but also inhibit tumor growth; Health is good. The structural formula of vitamin A is as follows: [0003] [0004] Vitamin A is insolu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/74
CPCG01N30/02G01N30/06G01N30/34G01N30/74G01N2030/027
Inventor 钟晓玲李勇白洁于多田敏卿徐希平
Owner SHENZHEN AUSA PHARM CO LTD
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