Nano-drug as well as preparation method and medical application thereof
A technology of nano-medicine, cholesterol, applied in the field of medicine
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Embodiment 1
[0088] (1) Dissolve egg yolk lecithin, cholesterol, dioleoylphosphatidylserine (DOPS), distearoylphosphatidylethanolamine-polyethylene glycol 2000 (DSPE-PEG2000) at a molar ratio of 36:20:20:4 In an organic solvent, a solution with a concentration of 10 mg / mL was obtained, and was rotated at 40°C at 120 rpm to remove the organic solvent to obtain a phospholipid film. The organic solvent was a mixture of chloroform and methanol with a volume ratio of 70:30;
[0089] (2) Dissolve 10 mg of bisphosphorus (LuH-6) in 1 mL of water to form an aqueous solution, dissolve the phospholipid film in the aqueous solution, the weight ratio of the aqueous solution to the phospholipid film is 1:10, and then mix at 25 ° C for 2 hours to obtain drug-loaded mixture;
[0090] (3) The modified transferrin receptor nucleic acid aptamer SEQ ID NO: 6, whose 5' end is modified with cholesterol to ensure that it can be inserted into the phospholipid hydrophobic layer, and the 3' end is modified with inv...
Embodiment 2
[0095] (1) Dissolve soybean lecithin, cholesterol, dipalmitoylphosphatidylserine (DPPS), distearoylphosphatidylethanolamine-polyethylene glycol 2000 (DSPE-PEG2000) at a molar ratio of 45:20:10:5 In an organic solvent, a solution with a concentration of 20 mg / mL was obtained, and the phospholipid film was obtained by rotary evaporation at 40° C. at 150 rpm to remove the organic solvent. The organic solvent was a mixture of chloroform and methanol with a volume ratio of 50:50;
[0096] (2) Dissolve 20mg of bisphosphorus (LuH-6) in 1mL of water to form an aqueous solution, dissolve the phospholipid film in the aqueous solution, the weight ratio of the aqueous solution to the phospholipid film is 1:5, and then mix at 25°C for 3h to obtain drug-loaded mixture;
[0097] (3) The modified transferrin receptor nucleic acid aptamer SEQ ID NO: 6, whose 5' end is modified with cholesterol to ensure that the nucleic acid can be inserted into the phospholipid hydrophobic layer, and the 3' e...
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