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Preparation method of bifidobacterium microcapsules based on endogenous emulsification

A technology of bifidobacteria and microcapsules, which is applied in the field of preparation of bifidobacteria microcapsules, can solve the problems of too large particle size and fixed-point release, and achieve the effect of less crack distribution, easy control and dense surface

Pending Publication Date: 2021-01-29
ZHENGZHOU UNIV OF IND TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the encapsulation of probiotics by mixed wall materials and coated wall materials can protect the bacterial viability to a certain extent, but both have shortcomings, such as too large particle size, unable to release at fixed points, etc.

Method used

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  • Preparation method of bifidobacterium microcapsules based on endogenous emulsification

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] combine figure 1 , a preparation method based on endogenous emulsified bifidobacterium microcapsules, comprising the steps of:

[0036] S1. Activation of strains and preparation of seed solution: Subculture bifidobacteria stored at low temperature in 37°C constant temperature MRS liquid medium, and inoculate in MRS solid medium for 24 hours after dilution of the three-generation cultured bacteria solution. -48h, pick a single colony and inoculate it into the MRS liquid medium to activate the seed liquid of passage 2-3, centrifuge and wash it, and then obtain the concentrated bacterial liquid and store it at low temperature for later use;

[0037] S2. Preparation of microcapsules: use sodium alginate and calcium carbonate as wall materials, and bifidobacteria as capsule materials, activate the concentrated bacterial solution obtained in S1 for 2-3 generations and collect the bacteria sludge, and sterilize the sodium alginate Mix the solution, bacteria slime and calcium ...

Embodiment 2

[0040] On the basis of embodiment 1, a kind of preparation method based on the bifidobacterium microcapsule of endogenous emulsification is characterized in that: comprise the steps:

[0041] S1: Activation of strains and preparation of seed liquid: The bifidobacteria preserved at low temperature were subcultured in 37°C constant temperature MRS liquid medium, and the bacteria liquid after the third-generation culture was diluted and inoculated in MRS solid medium for 36 hours. , pick a single colony and inoculate it into the MRS liquid medium to activate the seed liquid of passage 2-3, centrifuge and wash it, and then obtain the concentrated bacterial liquid and store it at low temperature for later use;

[0042] Liquid MRS medium: peptone 10.0g, yeast extract 5.0g, glucose 20.0g, ethyl sodium 5.0g, beef 10.0g, Tween 80 1.0ml, diamine hydrogen citrate 2.0g, dipotassium hydrogen phosphate 2.0g, MgSO 4 ·7H 2 O 0.2g, MnSO 4 ·H 2 O 1.0g, distilled water 1000mL, pH6.2-6.4, ster...

Embodiment 3

[0052] On the basis of Example 2, the morphology observation of microcapsules and the analysis of particle size: get 1 drop of the dispersed solution of microcapsules with glass rod, place on glass slide, carry out morphology observation with optical microscope, measure with micrometer The particle diameter of the capsules, count more than 100, take the average value, put the prepared microcapsules into a vacuum freeze dryer after pre-freezing, take them out after 24h, measure the entrapment rate and The number of viable bacteria, the ratio of bacteria slime and protective agent is 1:2.5, the concentration of trehalose in the protective agent is 10%, the concentration of skim milk is 15%, the concentration of glycerin is 3%, and the concentration of L-cysteine ​​is 2%, the embedding rate reaches the highest is 84.5%, and the number of live bacteria is at least 7.88×10 9 cfu / mL, the structure of microcapsules after freeze-drying was observed by scanning electron microscope.

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Abstract

The invention discloses a preparation method of bifidobacterium microcapsules based on endogenous emulsification. The preparation method comprises the following steps of S1, activation of strains andpreparation of a seed solution, S2, preparation of microcapsules, S3, secondary coating of microcapsules, and S3, preparation of microcapsules embedded for the second time so that the tolerance of thalli in freeze-dried bacterial powder to an acid environment is effectively improved, and the freeze-dried bacterial powder has good enteric solubility; the wrapped probiotics can be released in time in the intestines.

Description

technical field [0001] The invention belongs to the technical field of microcapsule products, and in particular relates to a preparation method of bifidobacterium microcapsules based on endogenous emulsification. Background technique [0002] A microcapsule is a microscopic container or package with polymeric or inorganic walls. Microcapsule granulation technology is to embed and seal solid, liquid or gas in a microcapsule to become a solid particle product. [0003] Bifidobacterium is an important intestinal beneficial microorganism. Probiotics can be roughly divided into categories: Bifidobacterium and Lactobacillus. Bifidobacteria include Bifidobacterium longum, Bifidobacterium breve, Bifidobacterium adolescent, etc. The main functions of the probiotic-like group are: to improve the immunity of the organism by protecting the body from the infection of pathogenic bacteria; to promote the peristalsis of the intestinal tract by inhibiting the growth and reproduction of path...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/135A23L33/125A23L33/16A23P10/30C12N1/04C12N1/20C12R1/01
CPCA23L33/135A23L33/125A23L33/16A23P10/30C12N1/04C12N1/20A23V2200/00A23V2200/3204A23V2400/517
Inventor 王庆卫
Owner ZHENGZHOU UNIV OF IND TECH
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