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Hydrolyzed protein liposome as well as preparation method and application thereof

A technology for hydrolyzing keratin and protein lipids, which can be used in pharmaceutical formulations, cosmetic preparations, cosmetic preparations, etc. It can solve the problems of low concentration of hydrolyzed keratin, unstable active properties, surface denaturation, etc., and increase the concentration of active substances , Improve encapsulation efficiency, improve the effect of stability

Active Publication Date: 2021-02-05
JINAN UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, if the concentration of hydrolyzed keratin in the solution is too low, it is easy to cause subunit dissociation and surface denaturation, and the activity in the solution is unstable and poor in stability.

Method used

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  • Hydrolyzed protein liposome as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The preparation of embodiment 1 hydrolyzed keratin

[0041] The preparation of hydrolyzed keratin of the present invention comprises the following steps:

[0042] S1. Wash and dry wool tops, put them into molten urea containing 7 times the weight of wool, and dissolve and react at 140°C for 1 hour;

[0043] S2, add 10 times the volume of NaHSO 4 Dissolve the aqueous solution, stir for 1 hour at the same time, use a cut-off 4-6kD dialysis bag, dialyze in distilled water for 30 hours, replace the distilled water every 15 hours, filter, filter and collect the filter residue, and dry to obtain hydrolyzed keratin with a molecular weight of 4-6kD;

[0044] S3, add 10 times the volume of Na to the filtrate 2 CO 3 Carry out alkaline hydrolysis, stir for 2 hours at the same time, use a cut-off quality 2-4kD dialysis bag, dialyze in mercaptoethanol 0.08% aqueous solution for 30 hours, replace mercaptoethanol 0.08% aqueous solution every 15 hours, filter and collect the filter ...

Embodiment 2

[0046] Embodiment 2 a kind of hydrolyzed liposome proteosome

[0047] The components and parts by weight of the hydrolyzed proteoliposome of Example 2 of the present invention are as shown in Table 1:

[0048] 40 parts of hydrolyzed keratin, 15 parts of ceramide-3, 150 parts of sitosterol, 5 parts of sodium deoxycholate, 110 parts of egg yolk lecithin, 13 parts of sesamol, 4 parts of carbomer, 1 part of essence, 1200 parts of trehalose parts, 120 parts of glycine;

[0049] The molecular weight of the hydrolyzed protein is a mixture of 4-6KD hydrolyzed protein and 2-4KD hydrolyzed protein; the weight fraction ratio of 4-6KD hydrolyzed protein and 2-4KD hydrolyzed protein is 1:0.6;

[0050]The preparation method of the hydrolyzed liposome specifically comprises the following steps:

[0051] 1) dissolving sitosterol and egg yolk lecithin in tert-butanol in a water bath at 85°C, and rotating to evaporate to obtain a uniform lipid film;

[0052] 2) Take hydrolyzed keratin, ceram...

Embodiment 3

[0055] Embodiment 3 a kind of hydrolyzed liposome proteosome

[0056] The components and parts by weight of the hydrolyzed proteoliposome of Example 3 of the present invention are as follows:

[0057] 45 parts of hydrolyzed keratin, 20 parts of ceramide-3, 130 parts of cholesterol, 5 parts of sodium cholate, 130 parts of sphingomyelin, 15 parts of tocopheryl acetate, 4 parts of carbomer, 1 part of essence, 1300 parts of trehalose , 130 parts of glycine;

[0058] The molecular weight of the hydrolyzed protein is a mixture of 4-6KD hydrolyzed protein and 2-4KD hydrolyzed protein; the weight fraction ratio of 4-6KD hydrolyzed protein and 2-4KD hydrolyzed protein is 1:0.7;

[0059] The preparation method of the hydrolyzed liposome specifically comprises the following steps:

[0060] 1) dissolving cholesterol and sphingomyelin in tert-butanol in a water bath at 75°C, and rotary evaporating to obtain a uniform lipid film;

[0061] 2) Take hydrolyzed keratin, ceramide-3, cholester...

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Abstract

The invention belongs to the technical field of cosmetics, and particularly relates to hydrolyzed protein lipidosome as well as a preparation method and application thereof. The hydrolyzed protein lipidosome comprises the following components of cosmetic active components, lipidosome framework components, object components and a freeze-drying protective agent, wherein the cosmetic active components include hydrolyzed keratin and ceramide; the liposome framework components include sterol and phospholipid; and the object components include a surfactant, an antioxidant, a thickener and essence. The lipidosome is used as a carrier of the hydrolyzed keratin, so that the stability of the hydrolyzed keratin can be improved on one hand, and the transdermal absorption of the hydrolyzed keratin canbe promoted on the other hand. Meanwhile, the concentration of active substances is continuously increased in a process of being in contact with the skin, so that the active substances act on cells more sufficiently, the moisture and elasticity of the skin are increased, and the moisturizing performance is high.

Description

technical field [0001] The invention relates to the technical field of cosmetics, in particular to a hydrolyzed proteosome and a preparation method and application thereof. Background technique [0002] The skin is the largest organ of the human body, and the skin barrier refers to the "brick wall structure" composed of stratum corneum cells and cells, which together form a natural protective barrier for the human body. Radiation, pollution, and ultraviolet rays in modern life can inhibit the normal physiological metabolism of structural proteins and natural moisturizing factors in keratinocytes, reduce the water content of the stratum corneum, down-regulate the expression of related moisturizing factors in human skin keratinocytes, and trigger skin hydration Decreased ability, which in turn causes damage to the skin barrier function. [0003] Keratin (Keratin, KRT) is the main structural protein of epidermal cells, which belongs to the intermediate filament protein family ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/14A61K8/65A61K8/68A61K8/63A61K8/55A61K8/60A61K8/34A61K8/44A61K8/73A61K8/67A61K8/49A61Q19/00
CPCA61K8/14A61K8/65A61K8/68A61K8/63A61K8/553A61K8/60A61K8/345A61K8/44A61K8/733A61K8/676A61K8/678A61K8/347A61K8/498A61K8/4973A61Q19/00A61Q19/005A61K2800/522
Inventor 刘忠杨涛
Owner JINAN UNIVERSITY
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