Preparation method of lvsong peptide rhzomorph C

A technology of peptidocin and actinomycetes, applied in the field of biopharmaceuticals, can solve the problems of high cost, complex structure, low yield and the like
CN112359048AActive Publication Date: 2021-02-12ZHEJIANG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
ZHEJIANG UNIV
Publication Date
2021-02-12

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Abstract

The invention discloses a preparation method of lvsong peptide rhzomorph C, and belongs to the field of biological pharmacy. The preparation method comprises the following steps: taking actinomyces lschsoni DSM43766 as a starting strain, inactivating an acetylase gene luz27 by virtue of a gene inactivation technology, and enabling the nucleotide sequence of the acetylase gene luz27 to be shown asSEQ ID NO.2. The invention further discloses the application of the actinomyces lschsoni DSM43766 in preparation of the acetylase gene luz27. And then inoculating the constructed mutated strain of actinomyces lschsonii into a fermentation culture medium, carrying out fermentation culture, and separating and purifying a fermentation product to obtain the lschsonii peptide rhzomorph C. According tothe invention, the acetylase gene luz27 of actinomyces lschsonii is inactivated by a genetic engineering means to block the acetylation modification process of lschsonii bacteriocin C, so that the component lschsonii bacteriocin C with higher biological activity is specifically accumulated. The lvsong peptide bacteriocin C is specifically produced through a microbial fermentation method, and the method is green, economical and efficient and has industrial production prospects.
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Description

technical field

[0001] The invention relates to the field of biopharmaceuticals, in particular to a green, efficient and specific method for preparing the antiviral active molecule luzonin C. Background technique

[0002] Luzopeptin (luzopeptin) is a kind of double intercalation family non-ribosomal peptide compound produced by Actinomadura sp.DSM43766. The strain was isolated in 1980 by Japanese scientists from soil samples in Luzon, Philippines. Lusopeptide has good antitumor activity and antibacterial activity, and is also a highly effective HIV reverse transcriptase inhibitor.

[0003] Actinomyces madura luzon can produce A, B, C and other components under different fermentation conditions. The general structural formula of luzonin A~C is as formula (I),

[0004]

[0005] Lusopeptide A:R 1 =R 2 =CH 3 CO; Lusopeptin B:R 1 =H,R 2 =CH 3 CO; Lusopeptin C:R 1 =R 2 =H.

[0006] Lureotin C contains two 4-hydroxy-2,3,4,5-tetrahydropyridazine-3-carboxylic acid stru...

Claims

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