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Production method of molecularly imprinted composite paste electrode sensor for detecting ribavirin

An electrode sensor and molecular imprinting technology, which is applied to instruments, measuring devices, scientific instruments, etc., can solve the problems of difficulty in accurately detecting ribavirin, mutual interference of coexisting substances, and low ribavirin content, and achieve high affinity. The effect of stability and selectivity, easy surface and low cost

Active Publication Date: 2021-02-12
INNER MONGOLIA UNIV OF SCI & TECH
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AI Technical Summary

Problems solved by technology

[0003] At present, the methods for detecting ribavirin mainly include high-performance liquid chromatography, liquid chromatography-mass spectrometry, and ultraviolet spectrophotometry. The instruments of these methods are relatively expensive and require professional operation, and the detection period is long, which also limits their application.
In addition, due to the relatively low content of ribavirin in biological samples and the mutual interference of coexisting substances, it is difficult to accurately detect ribavirin

Method used

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preparation example Construction

[0030] Further, the present invention provides a method for preparing a molecularly imprinted composite paste electrode sensor for detecting ribavirin, which includes the following steps:

[0031] Step 1), preparation of nano-dysprosium nitride / nano-strontium titanate / graphene oxide composite paste electrode: the composite paste electrode uses nano-dysprosium nitride, nano-strontium titanate and graphene oxide as conductive materials, and 1-aminopropyl -3-methylimidazolium hexafluorophosphate as an adhesive, with triethanolamine and ethanol as a dispersant;

[0032]Step 2), preparing ribavirin molecularly imprinted polymer: in an oxygen-free atmosphere, in an aqueous solution, using aconitic acid as a functional monomer, methylenebisacrylamide as a crosslinking agent, and azobisiso Heptanitril is used as an initiator, and ribavirin is used as a template for cross-linking polymerization to prepare ribavirin molecularly imprinted polymers;

[0033] Step 3), preparing a composit...

Embodiment 1

[0056](1) Preparation of nano-dysprosium nitride / nano-strontium titanate / graphene oxide composite paste electrode: In an agate mortar, add respectively, nano-dysprosium nitride: 24g, 1-aminopropyl-3-methylimidazole hexa Fluorophosphate: 6g, nano-strontium titanate: 6g, graphene oxide: 5g, triethanolamine: 4g, ethanol: 5g, grind evenly to get the mixture carbon paste; In a Φ5mm glass tube, compact, dry, grind with polishing powder, polish, and wash with deionized water. After testing, the conductivity of the composite paste electrode is 5-6 times higher than that of ordinary carbon paste electrodes.

[0057] (2) Preparation of ribavirin molecularly imprinted polymer: In the reactor, add deionized water: 31g, aconitic acid: 9g, ribavirin: 4g, methylenebisacrylamide: 5g, Azobisisoheptanonitrile: 1.0g, stirred and dissolved, deoxygenated by argon for 10 minutes, in an oxygen-free atmosphere, stirred and reacted at 65±2°C for 7 hours, separated from solid and liquid, washed several...

Embodiment 2

[0060] (1) Preparation of nano-dysprosium nitride / nano-strontium titanate / graphene oxide composite paste electrode: In an agate mortar, add nano-dysprosium nitride: 49g, 1-aminopropyl-3-methylimidazole hexa Fluorophosphate: 14g, nano-strontium titanate: 11g, graphene oxide: 12g, triethanolamine: 6g, ethanol: 8g, grind evenly to get the mixture carbon paste; In a Φ5mm glass tube, compact, dry, grind with polishing powder, polish, and wash with deionized water. After testing, the conductivity of the composite paste electrode is 5-6 times higher than that of ordinary carbon paste electrodes.

[0061] (2) Preparation of ribavirin molecularly imprinted polymer: In the reactor, add deionized water: 15g, aconitic acid: 4g, ribavirin: 2.5g, methylenebisacrylamide: 3g , azobisisoheptanonitrile: 0.35g, stirred and dissolved, deoxygenated by argon for 10min, in an oxygen-free atmosphere, stirred and reacted at 65±2°C for 8h, separated from solid and liquid, washed several times, and drie...

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Abstract

The invention relates to a production method of a molecularly imprinted composite paste electrode sensor for detecting ribavirin. The production method comprises the following steps: producing a nanodysprosium nitride / nano strontium titanate / graphene oxide composite paste electrode; preparing a ribavirin molecularly imprinted polymer: in an oxygen-free atmosphere, taking aconitic acid as a functional monomer, taking methylene bisacrylamide as a cross-linking agent, taking azobisisoheptonitrile as an initiator and taking ribavirin as a template to carry out a cross-linking polymerization reaction to prepare the ribavirin molecularly imprinted polymer; and producing a composite paste electrode sensor: dropwise adding a ribavirin molecularly imprinted polymer modification liquid into the nano dysprosium nitride / nano strontium titanate / graphene oxide composite paste electrode by adopting a dispensing method, volatilizing a solvent, and removing template molecules to obtain the composite paste electrode sensor. According to the method, a molecular imprinting technology, a layer-by-layer self-assembly method and a dispensing method are combined, and the produced molecular imprinting composite paste electrode sensor is good in response performance, good in selectivity and high in sensitivity.

Description

technical field [0001] The invention relates to the field of electrochemical sensors, in particular to a preparation method of a molecularly imprinted composite paste electrode sensor for detecting ribavirin. Background technique [0002] Ribavirin (ribavirin, Ribavirin), this product is 1-β-D-ribofuranosyl-1H-1,2,4-triazole-3-carboxamide, the molecular formula is C 8 h 12 N 4 o 5 , the molecular weight is 244.21. Ribavirin is a broad-spectrum antiviral drug that can inhibit the growth of a variety of DNA or RNA viruses. It is widely used clinically in the treatment of chronic hepatitis C, influenza A or B, herpes zoster, virus Rhinitis, pharyngitis, acute upper respiratory tract infection, etc. However, animal experiments have found that long-term use of ribavirin can lead to problems such as poisoning, immunosuppression, and drug residues, which are harmful to human health. Therefore, it is of great significance to develop a rapid and accurate detection method for ri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/30G01N27/327
CPCG01N27/26G01N27/30G01N27/3278
Inventor 李冬梅田忠贞
Owner INNER MONGOLIA UNIV OF SCI & TECH
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