Application of pomfret oil or component extracts thereof in preparation of breast milk fat substitutes
A technology to replace fat and pomfret, applied in application, edible oil/fat, dairy products, etc., can solve the problem of low similarity, achieve high similarity, improve hardness, and reduce the occurrence of constipation and intestinal diseases.
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Embodiment 1
[0053] Example 1: Golden Pomfret Oil
[0054] Chloroform-methanol method was used to extract golden pomfret oil (oval pomfret fish oil), and fresh golden pomfret liver was freeze-dried and crushed. Take 10g of crushed powder, add 100mL of methanol, shake, then add 200mL of chloroform, ultrasonic for 30min, take clear liquid after suction filtration, add 100mL of sodium chloride solution with a mass fraction of 0.88%, centrifuge the mixed solution at 4500rpm After 10 minutes, absorb the lower layer, place it in a round bottom flask, and remove the solvent by rotary evaporation to obtain golden pomfret oil. The measured total palmitic acid, sn-2 palmitic acid, OPO and OPL accounted for 23.52%, 37.25%, 15.84% and 25.67% of the total triglyceride content in golden pomfret oil respectively. 52.79% of the total palmitic acid content. And the content of OPL is higher than that of OPO, so breast milk substitute fat similar to Chinese breast milk fat can be formulated without adding ...
Embodiment 2
[0055] Embodiment 2: freshwater white pomfret oil
[0056] The freshwater pomfret oil was extracted with n-hexane as solvent. Take 10 g of the pulverized powder, add 100 mL of n-hexane, and ultrasonicate for 30 minutes. After suction filtration, put the clear liquid in a round bottom flask, and remove the solvent by rotary evaporation to obtain freshwater pomfret oil. The total palmitic acid, sn-2-position palmitic acid, OPO and OPL accounted for 25.26%, 36.96%, 26.39% and 19.38% of the total triglycerides in freshwater pomfret oil were measured. The calculation shows that the sn-2 palmitic acid accounts for 48.77% of the total palmitic acid content. Although the content of OPL is slightly lower than that of OPO and golden pomfret oil, it is higher than that of other fish oils. Therefore, a small amount of OPL structural fat can be added to adjust the ratio of OPL to OPO to replace breast milk fat. deployment.
Embodiment 3
[0057] Embodiment 3: a kind of preparation method of the fractionation product of gold pomfret oil
[0058] The golden pomfret oil obtained in Example 1 was fractionated by solvent method, the solvent used was acetone, the ratio of golden pomfret oil to solvent (w / v) was 1:8, the fractionation temperature was -25°C, and the fractionation time was 16h, to obtain Golden Pomfret Oil Fraction. The contents of total palmitic acid, sn-2 palmitic acid, OPO and OPL in the golden pomfret oil fraction were 27.82%, 45.37%, 20.28% and 29.46%, respectively. The calculation shows that the sn-2 palmitic acid accounts for 54.36% of the total palmitic acid content.
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