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Bacillus paralicheniformis ZYGT1811 and application thereof

A technology of Bacillus licheniformis and bacterial liquid, applied in the application, bacteria, fungicides and other directions, can solve the problems of few good varieties, aggravating the resistance of soil pesticide residues to pathogenic bacteria, abuse of chemical agents, overdose use, etc. Effect

Active Publication Date: 2021-02-26
INST OF PLANT PROTECTION GANSU ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The chemical control of this disease has not yet found a specific agent, and currently it mainly relies on common fungicides, such as thiram, mancozeb, carbendazim, etc., but its control effect is not very satisfactory
In order to pursue the prevention and control effect and obtain greater economic benefits in production, the phenomenon of chemical agent abuse and overdose use occurs from time to time, which easily aggravates pesticide residues in the soil, induces drug resistance of pathogenic bacteria, and has serious negative effects on food safety and environmental safety. influences
Physical control methods such as steam disinfection, hot water disinfection, and solar disinfection are time-consuming, laborious and costly, and are not applicable in actual production
The selection of disease-resistant varieties is the most economical and effective means to prevent and control the disease, but the breeding cycle of excellent disease-resistant varieties is relatively long. At present, there are relatively few excellent varieties resistant to Fusarium wilt selected in my country

Method used

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  • Bacillus paralicheniformis ZYGT1811 and application thereof
  • Bacillus paralicheniformis ZYGT1811 and application thereof
  • Bacillus paralicheniformis ZYGT1811 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Isolation and screening of biocontrol bacteria ZYGT1811 and determination of its inhibitory effect on common plant pathogens

[0026] 1.1 Isolation of biocontrol bacteria ZYGT1811

[0027] Soil samples were collected from the salinized plot in Gaotai County, Zhangye City, Gansu Province. The five-point sampling method was used for sampling. The soil samples were mixed and brought back in sealed bags. The soil clods and plant residues were removed with a mesh sieve and air-dried at room temperature. Weigh 10g soil sample and put it into a triangular flask filled with 90mL sterilized water to make 10 -1 Concentration of soil suspension, let it stand until the soil particles settle, draw 1mL supernatant and transfer it into a test tube filled with 9mL sterilized water, and perform gradient dilution in sequence to obtain a final concentration of 10 -5 soil suspension. choose 10 -3 , 10 -4 and 10 -5 200 μL of each of the three concentrations of the suspension...

Embodiment 2

[0037] Antibacterial and life-promoting activity assay of embodiment 2 biocontrol bacteria ZYGT1811

[0038] 1. Detection of enzyme production activity in vitro

[0039] 1.1 Detection of amylase production ability

[0040] Inoculate the biocontrol bacteria ZYGT1811 bacterial strain in LB solid medium containing soluble starch (10g soluble starch, 5g yeast powder, 10g peptone, 10g NaCl, 15g agar, 1000mL water), culture at 28°C for 3-5d, and use Gram The iodine solution submerges the amylase detection medium, and a clear transparent hydrolysis circle can be seen around the amylase-producing colonies. Observe and record the diameters of colonies and transparent circles, and judge the amylase-producing ability of the strains by calculating the difference.

[0041] 1.2 Detection of Protease Production Ability

[0042] Inoculate the bacterial strain ZYGT1811 of biocontrol bacteria in LB solid medium containing skimmed milk powder (yeast powder 5g, peptone 10g, NaCl 10g, agar 15g,...

Embodiment 3

[0065] Example 3 Pot experiment of biocontrol bacterium ZYGT1811 fermented liquid controlling cabbage wilt

[0066] 1.1 Preparation of biocontrol bacteria ZYGT1811 fermentation broth

[0067] Strain ZYGT1811 was inoculated on LB solid medium and activated at 28°C, then transferred to a 100mL Erlenmeyer flask containing 50mL LB liquid medium, and cultured at 28°C for 24 hours with shaking at 150r / min to obtain seed liquid. Inoculate the seed solution in the fermentation medium at a volume ratio of 1:100 After culturing on a shaker at 28°C and 150r / min for 24 hours, the fermentation broth of biocontrol bacteria ZYGT1811 was obtained.

[0068] 1.2 Determination of potted plant control effect of biocontrol bacteria ZYGT1811 fermented liquid on cabbage wilt

[0069] Inoculate the cabbage wilt pathogen Fusarium oxysporum into PLB liquid medium (potato 200g / L, lactose 20g / L, water 1000mL), culture on a shaker for 3 days, collect the bacterial liquid, and dilute the bacterial liqui...

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Abstract

The invention provides Bacillus paralicheniformis ZYGT1811. The preservation number of the Bacillus paralicheniformis ZYGT1811 is CCTCC M 2020759. The biocontrol bacterium ZYGT1811 can significantly inhibit the growth of cabbage wilt pathogenic bacteria, so that the phenomena of expansion deformation and branch internode shortening of pathogenic bacteria hyphae occur. The strain can produce amylase, protease, pectinase, beta-1, 3-glucanase, siderophore, NH3 and IAA, has a nitrogen fixation effect, and does not have the ability to dissolve phosphorus and potassium. The strain has an inhibitoryeffect on common plant pathogens (rhizoctonia solani, sclerotinia sclerotiorum, phytophthora capsici, fusariumverticillioides, F.solani and F.graminearum), and has certain broad-spectrum activity. Thecontrol effect of the strain on fusarium oxysporum is 57.73%, and the difference between the control effect of the strain and the control effect of a control group is significant.

Description

technical field [0001] The invention belongs to the technical field of plant fusarium wilt biological control, and in particular relates to a strain of bacillus paralicheniformis and its application, especially the application in preparing bacterial agents or medicines for preventing and treating cabbage wilt. Background technique [0002] Cabbage wilt is a typical soil-borne disease caused by Fusarium oxysporum, which can remain in the soil for several years or even decades, seriously affecting the quality and yield of cabbage. In recent years, the disease has occurred in various cabbage planting areas in my country. With the increase of the planting area and the extension of the planting years, the disease is becoming more and more serious. The chemical control of this disease has not yet found a specific agent, and currently it mainly relies on common fungicides, such as thiram, mancozeb, carbendazim, etc., but its control effect is not very satisfactory. In order to pur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01P3/00A01P21/00C12R1/07
CPCA01N63/22C12N1/20C12N1/205C12R2001/07
Inventor 刘永刚陈云云徐生军郁继华李惠霞张海英
Owner INST OF PLANT PROTECTION GANSU ACAD OF AGRI SCI
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