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Paracoccus for degrading N, N-dimethylformamide and application thereof in wastewater treatment

A technology for wastewater treatment and paracoccus, which is applied in the field of biological treatment of organic pollutants, can solve the problem of high operating costs, and achieve the effects of high treatment efficiency, high-efficiency denitrification and denitrification, and high-efficiency DMF degradation.

Active Publication Date: 2021-02-26
NANJING UNIV OF SCI & TECH +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Aiming at the current biological process for treating waste water containing pollutant DMF all needs to be carried out under oxygen-consuming conditions, and the problem of high operating costs, the invention provides a paracoccus (Paracoccus) bacterial strain that efficiently degrades DMF, and the bacterial strain can utilize DMF As an electron donor, nitrate nitrogen is used as an electron acceptor for denitrification and denitrification reaction, and the degradation of DMF is achieved while anoxic denitrification and denitrification

Method used

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  • Paracoccus for degrading N, N-dimethylformamide and application thereof in wastewater treatment
  • Paracoccus for degrading N, N-dimethylformamide and application thereof in wastewater treatment
  • Paracoccus for degrading N, N-dimethylformamide and application thereof in wastewater treatment

Examples

Experimental program
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Effect test

Embodiment 1

[0056] Screening, isolation and identification of DMF-degrading Paracocci.

[0057] (1) Screening and isolation of strains

[0058] Sampling 10mL from the activated sludge that has been used to treat DMF wastewater for a long time was added to 100mL sterile physiological saline (0.85% sodium chloride solution), stirred evenly, and left to stand for two hours. Take 1 mL of the supernatant and add it to 50 mL of inorganic salt liquid medium, and enrich and cultivate it in a constant temperature shaking incubator for three days (28 ° C ~ 32 ° C, 180 rpm). Bacterial normal saline (0.85% sodium chloride solution) gradient dilution to 10 -4 -10 -10 times.

[0059] To prepare inorganic salt agar solid medium plates, spread 20 μL of the diluted culture solution on the inorganic salt agar solid medium plates, and place them in a biochemical incubator at 28°C to 32°C for three days. Single colonies with obvious differences on the petri dish were selected, purified and cultivated by ...

Embodiment 2

[0074] Application of strain Paracoccus sp.NJUST48 in the treatment of wastewater containing DMF and nitrate nitrogen.

[0075] Inoculate Paracoccus sp.NJUST48 into LB liquid medium containing 6.8mM DMF, adjust the pH to 6.5-7.5, and culture it on a shaking table at 180 rpm at 28°C-32°C to enrich the strain of NJUST48. After entering the logarithmic growth phase (about 48 hours), the obtained bacterial liquid was centrifuged with an ultra-low temperature centrifuge for 10 minutes (4° C., 6000 rpm) to obtain deposited bacterial cells. Resuspend in sterilized inorganic salt liquid medium, centrifuge, repeat washing three times, resuspend bacteria in sterile liquid inorganic salt medium, and obtain seed liquid when OD600 is 1.5-2.0.

[0076] Add 100mL of inorganic salt liquid medium containing DMF with an initial concentration of 13.7±0.9mM and sodium nitrate with an initial concentration of 17.6±1.9mM into a 120mL serum bottle as simulated wastewater, and continuously inject hig...

Embodiment 3

[0079] Application of strain Paracoccus sp.NJUST48 in the treatment of wastewater containing DMF and nitrate nitrogen under different pH conditions.

[0080] Prepare 100 mL of inorganic salt liquid culture medium containing DMF with an initial concentration of 13.5 ± 1.8 mM, an initial concentration of nitrate nitrogen of 17.6 ± 2.0 mM, and an initial pH of 5.0, 6.0, 7.0, 8.0, and 9.0 as simulated wastewater, and add it to 120 mL of serum In the bottle, and continuously feed high-purity helium to remove the dissolved oxygen in the serum bottle and wastewater, and construct an anoxic denitrification system. Subsequently, the serum bottle was sealed with a butyl rubber stopper and an aluminum cap, autoclaved at 121° C. for 20 minutes, and left to stand at room temperature. Inoculate the above seed solution into serum bottles with an inoculum amount of 5% to 10%, culture on a shaking table at 28°C to 32°C and 180 rpm, and monitor the concentration changes of DMF and nitrate nitro...

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Abstract

The invention discloses a paracoccus for efficiently degrading N, N-Dimethylformamide (DMF) and application thereof, belonging to the field of biological treatment of organic pollutants in the environment. In the application, activated sludge used for treating DMF for a long time is used as a bacteria source, an inorganic salt culture medium using DMF as a carbon source is used as a screening culture medium, and separation and purification are performed to obtain the paracoccus which can efficiently degrade DMF and perform nitrogen removal by denitrification at the same time, wherein the collection number is CCTCC NO: M2020683. The paracoccus can perform anoxic nitrogen removal by denitrification by using DMF as an electron donor so to realize mineralization degradation of DMF, and the paracoccus has efficient organic matter degradation capacity and denitrification capacity, and is suitable for removal treatment of high-concentration nitrate nitrogen and refractory organic pollutants in wastewater.

Description

technical field [0001] The invention belongs to the technical field of biological treatment of organic pollutants, and relates to a strain of Paracoccus that degrades N,N-dimethylformamide, in particular to a strain that takes refractory organic pollutant N,N-dimethylformamide as an electron Paracocci as donors for denitrification and their use in wastewater treatment. Background technique [0002] N,N-Dimethylformamide (N,N-dimethylformamide, DMF) is a synthetic organic polar solvent, which is widely used due to its good water miscibility and the ability to dissolve different kinds of organic substances. In chemical, pharmaceutical and textile industries. It is reported that more than 5000 tons of DMF are discharged or transferred to the environment every year. DMF has a stable structure, is not easy to be hydrolyzed or photolyzed, and is not easy to be degraded by environmental microorganisms. It is easy to accumulate and transfer in the natural environment. It has the c...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/16
CPCC12N1/20C02F3/34C02F3/348C02F2101/16C02F2101/163C12R2001/01C12N1/205
Inventor 沈锦优王静张洪玲迟强江心白陈丹
Owner NANJING UNIV OF SCI & TECH
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