Genetically engineered bacterium for producing N-acetylglucosamine and application of genetically engineered bacterium

A technology of genetically engineered bacteria and acetylamino, applied in the field of genetic engineering, can solve problems such as limiting the conversion rate of N-acetylglucosamine, and achieve the effects of strengthening industrial production potential, improving conversion rate, and improving synthesis efficiency.
CN112430562AActive Publication Date: 2021-03-02TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
Publication Date
2021-03-02

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Abstract

The invention relates to the technical field of genetic engineering, in particular to a genetically engineered bacterium for producing N-acetylglucosamine based on metabolic division of a composite carbon source and application of the genetically engineered bacterium. By constructing an enhanced N-acetylglucosamine synthesis pathway, knocking out a 6-phosphofructokinase gene and directionally mutating a glycerokinase expression gene, metabolic division is carried out on the utilization of a glycerol-glucose composite carbon source, so that glucose is mainly used for producing a target productN-acetylglucosamine, glycerol is mainly used for cell growth, and the yield of the Nacetylglucosamine is increased. Therefore, the synthesis efficiency of the N-acetylglucosamine is improved. After the mixed carbon source culture medium is subjected to shake-flask fermentation for 48 hours, the yield of the Nacetylglucosamine reaches 107.8 g / L at most, the conversion rate of the composite carbon source reaches 49% at most, compared with a glucose utilization strain, the yield is increased by 151.7%, the conversion rate is increased by 60.2%, and the method has high industrial production potential.
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Description

Technical field:

[0001] The invention relates to the technical field of genetic engineering, in particular to a genetically engineered bacterium for producing N-acetylglucosamine based on complex carbon source metabolism and division of labor and its application. Background technique:

[0002] N-acetylglucosamine is a functional monosaccharide that plays an important role in the repair and health care of bones and joints, and relieves osteoarthritis. Therefore, it has a broad market application demand as health care products and medicines. The traditional production method is mainly to extract from shrimp shells and crab shells through acid hydrolysis, which has problems such as environmental pollution and easy allergies. In recent years, with the rapid development of metabolic engineering, reports on the production of N-acetylglucosamine by microbial fermentation are emerging. At present, the fermentation production of Escherichia coli, Bacillus subtilis, Corynebacterium g...

Claims

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