Ester compound in purslane as well as extraction and separation method and application thereof

An ester compound and separation method technology, applied in the field of an ester compound in purslane and its extraction and separation, can solve the problem of low structural novelty, achieve environmental protection of the process method, significant anti-tumor and anti-choline ester Enzyme activity, simple operation method and rapid effect

Active Publication Date: 2021-03-12
LIAONING UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, many scholars have focused on the determination of the chemical components of purslane, pharmacodynamics and pharmacokinetics, etc. However, there has never been a separation of a new ester compound in purslane and analysis in

Method used

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  • Ester compound in purslane as well as extraction and separation method and application thereof
  • Ester compound in purslane as well as extraction and separation method and application thereof
  • Ester compound in purslane as well as extraction and separation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1 extracts and separates the ester compound and its extraction and separation method from purslane.

[0036] A kind of ester compound extracted from purslane, molecular formula is C 20 h 34 o 3 , named (7E, 9E)-6-oxoctadecyl-7,9-dienoic acid ethyl ester, the chemical structural formula is:

[0037]

[0038] Table 1 is the nuclear magnetic data of this new compound: 1 H-NMR with 13 C-NMR in DMSO.

[0039] Table 1. NMR data of the new compounds of the present invention.

[0040] serial number δ C

Types of δ H (J in Hz)

1 172.50 C=O 2 33.45 CH 2

2.25,t(7.02) 3 23.53 CH 2

1.48,m 4 23.53 CH 2

1.48,mr 5 39.24 CH 2

2.54,t(7.50) 6 200.10 C=O 7 128.05 CH 6.10,d(15.54) 8 142.74 CH 7.18,m 9 128.96 CH 6.26,m 10 145.37 CH 6.26,m 11 32.38 CH 2

2.17,m 12 24.40 CH 2

1.48,m 13 24.56 CH 2

1.48,m 14 28.42 CH ...

Embodiment 2

[0050] Example 2 Anti-tumor effect of the new ester compound of the present invention.

[0051] 1 main material.

[0052] 1.1 Drugs and reagents.

[0053] The new ester compounds used in the experiment were prepared by the above method with a purity of 90% to 99%. They were accurately weighed and diluted with DMSO to the required solutions for the following dosage groups. DMEM high-glucose medium, fetal bovine serum (Hyclone Company of the United States); penicillin and streptomycin (Hangzhou Sijiqing Company).

[0054] 1.2 Cell lines.

[0055] Human colon cancer cell Caco-2, human breast cancer cell MCF-7, human gastric cancer cell BGC-823, human lung adenocarcinoma cell SPC-A1, human liver cancer cell BEL-7402, human cervical cancer cell Hela-229, ovarian cancer cell Ho-8910, human oral epidermoid carcinoma cells KB (Shanghai Cell Bank, Chinese Academy of Sciences).

[0056] 1.3 Grouping.

[0057] Divided into control group, experimental group and zero adjustment group ...

Embodiment 3

[0067] Example 3 The anticholinesterase effect of the new compound of the present invention.

[0068] 1. Main materials.

[0069] 1.1 Drugs and reagents.

[0070] The new compound used in the experiment was prepared by the above method with a purity of 90-99%. Sodium dihydrogen phosphate, disodium hydrogen phosphate (Sinopharm Chemical Reagent Co., Ltd.), physostigmine (Hanxiang Biotechnology), phosphorus 5,5'- Dithiobisnitrobenzoic acid (Dithiobisnitrobenzoic acid, DTNB, Shanghai Jinsui Biotechnology Co., Ltd.), acetylcholinesterase (AChE) and acetylthiocholine iodide (ATCI, Dalian Meilun Biotechnology Co., Ltd. Ltd.).

[0071] 1.2 Grouping.

[0072] Divided into negative control group, positive control group and experimental group, each group.

[0073] 2 Experimental methods.

[0074] 2.1 Sample preparation.

[0075] Precisely weigh the sample and 0.11 mg of physostigmine, respectively, and use methanol as the solvent to prepare five gradient concentrations of 2.5, 5.0...

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Abstract

The invention relates to the field of traditional Chinese medicine extraction and separation, in particular to a new compound extracted, separated and identified from a purslane medicinal material aswell as an extraction and separation method and application thereof. The invention provides (7E,9E)-6-oxooctadecyl-7,9-dienoic acid ethyl ester extracted from purslane as well as an extraction and separation method and application thereof. The ester compound is prepared by sequentially adopting alcohol decoction extraction, silica gel column chromatography, polyamide column chromatography, ODS medium-pressure column, Sephadex LH-20 purification, and liquid phase separation. The extraction separation method is simple, convenient, rapid and environment-friendly; the compound separated by the method is relatively high in purity; Pharmacological experiments prove that the obtained compound has anti-tumor and anti-cholinesterase effects, so that the new ester compound extracted from purslane and the salt and derivative thereof can be used as natural products to develop new traditional Chinese medicine, and have broad medical application prospects.

Description

technical field [0001] The present invention relates to the field of extraction and separation of traditional Chinese medicine, in particular to a new compound extracted, separated and identified from purslane medicinal material and its extraction and separation method, specifically an ester compound in purslane and its extraction and separation method and application . Background technique [0002] Purslane (Portulaca oleracea L.), also known as longevity dish, horse amaranth, is a plant of the family Amaranthaceae. Purslane is drought and waterlogging resistant, light and shade resistant, widely distributed, and rich in resources. It has attracted much attention as a wild plant for both medicine and food. The 2015 edition of "Pharmacopia of the People's Republic of China" recorded the dry aerial parts of purslane as medicine. , has the functions of clearing away heat and detoxification, cooling blood to stop bleeding, and stopping dysentery. [0003] Modern pharmacologic...

Claims

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Application Information

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IPC IPC(8): C07C69/738C07C67/48C07C67/52C07C67/56A61P35/00A61K31/231A23L33/105A23L33/12B01D15/18
CPCC07C69/738C07C67/48C07C67/52C07C67/56A61P35/00A23L33/105A23L33/12B01D15/1892C07B2200/09A23V2002/00A23V2200/308A23V2250/18A23V2250/21A23V2300/38A23V2300/02A23V2300/14A23V2300/50Y02A50/30
Inventor 成泽东陈以国齐姝博孙瑞英锡相顾莹莹
Owner LIAONING UNIV OF TRADITIONAL CHINESE MEDICINE
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