Alcaligenes faecalis GBW-HB1905 for efficiently removing COD (Chemical Oxygen Demand) in aquaculture wastewater and application of alcaligenes faecalis GBW-HB1905

A technology of GBW-HB1905 and Alcaligenes faecalis is applied in the field of microorganisms to achieve the effects of improving removal efficiency, ensuring stable operation, and broad application prospects

Inactive Publication Date: 2021-03-16
QINGDAO SHANGDE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Due to the good biodegradability of livestock and poultry breeding wastewater, biological treatment technology is often used to treat it. At present, biochemical treatment technology mainly includes aerobic treatment technology, anaerobic treatment technology, and anaerobic and aerobic combined technology; and At present, there are not many functional single bacteria that can efficiently and directly degrade COD in aquaculture wastewater, so finding functional strains that can efficiently degrade COD in aquaculture wastewater can not only promote and improve the removal efficiency of COD in aquaculture wastewater, but also make the discharge of COD in the effluent reach the standard , will further enhance and guarantee the impact resistance and operational stability of the wastewater system, which is of great value and significance

Method used

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  • Alcaligenes faecalis GBW-HB1905 for efficiently removing COD (Chemical Oxygen Demand) in aquaculture wastewater and application of alcaligenes faecalis GBW-HB1905
  • Alcaligenes faecalis GBW-HB1905 for efficiently removing COD (Chemical Oxygen Demand) in aquaculture wastewater and application of alcaligenes faecalis GBW-HB1905
  • Alcaligenes faecalis GBW-HB1905 for efficiently removing COD (Chemical Oxygen Demand) in aquaculture wastewater and application of alcaligenes faecalis GBW-HB1905

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1: Screening, isolation and identification of Alcaligenes faecalis GBW-HB1905

[0043] 1. Screening and purification of Alcaligenes faecalis GBW-HB1905

[0044] Take 15ml of mixed sewage to be treated from a municipal sewage plant, add it to 135mL of PBS buffer solution, oscillate for 20 minutes to fully mix the sample, centrifuge at 1000rpm for 1min, collect the sample supernatant, and set aside. Take 1 mL of the supernatant of the above sample, add it to an Erlenmeyer flask filled with 100 mL of high-salinity ammonium nitrogen degrading bacteria enrichment and screening liquid medium, culture at 28 °C, 180 rpm constant temperature shaker for 2 days, and enrich for 3 times. After gradient dilution of the cultured bacterial suspension, 100 μL was spread on the high-salinity ammonium nitrogen degrading bacteria enrichment and screening solid medium, and placed in a 28°C incubator for cultivation. After 48 hours, single colonies of different shapes were picked an...

Embodiment 2

[0049] Example 2: Growth determination and physiological and biochemical characteristics of Alcaligenes faecalis GBW-HB1905

[0050] 1. Growth determination of Alcaligenes faecalis GBW-HB1905

[0051] Inoculate the Alcaligenes faecalis GBW-HB1905 cultured on the slant into the improved NB medium, and culture it in a constant temperature shaker with a pH of 7.0-7.5 and a temperature of 28°C for 22 hours to prepare a GBW-HB1905 bacterial liquid, wherein every 1.0 hours Take a sample once, measure the absorbance value at OD600nm, and draw the growth curve. Such as figure 2 As shown, Alcaligenes faecalis GBW-HB1905 was in the growth retardation phase in the first 4 hours of culture, then entered the logarithmic growth phase, and entered the logarithmic growth phase after 14-16 hours of culture, and the number of bacteria reached 6.0×109CFU / mL, 16 -20h is the stable growth period, and then enters the decline period, thus completing the entire growth cycle.

[0052] 2. Physiolog...

Embodiment 3

[0058] Example 3: Salinity tolerance test of Alcaligenes faecalis GBW-HB1905

[0059] In order to test the survival performance of Alcaligenes faecalis GBW-HB1905 under high salinity conditions, its growth under different salinity conditions was analyzed and detected. Adjust the amount of sodium chloride in the NB medium to prepare medium with different salt concentrations, and then inoculate Alcaligenes faecalis GBW-HB1905 cultured on the slant into NB medium with different salt concentrations, at pH7.0-7.5, The culture solution of Alcaligenes faecalis GBW-HB1905 was obtained by culturing in a constant temperature shaker at 28°C for 18 hours, and the growth status of Alcaligenes faecalis GBW-HB1905 in media with different salt concentrations was detected. The results are shown in Table 2. GBW-HB1905 has good salt-tolerant growth characteristics, and can grow well in the salinity range of 15-35‰.

[0060] Table 2 Growth of Alcaligenes faecalis GBW-HB1905 in different salt con...

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Abstract

The invention provides alcaligenes faecalis GBW-HB1905 for efficiently removing COD (Chemical Oxygen Demand) in aquaculture wastewater and application of the alcaligenes faecalis GBW-HB1905. The alcaligenes faecalis GBW-HB1905 is classified and named as Alcaligenes faecalis, the preservation number of the alcaligenes faecalis is CGMCC No.20364, bacterial colonies of the alcaligenes faecalis are round and milky white, the diameter of the bacterial colonies is 1-2mm, the surfaces of the bacterial colonies are smooth, wet, slightly glossy, relatively flat, slightly convex in the middles and opaque, edges are neat, and no halos exist; and the optimum growth temperature is 25-32 DEG C, the pH value is 7.5-8.5, and the alcaligenes faecalis GBW-HB1905 has wide salt tolerance and can normally growunder the salt concentration of 15 permillage-35 permillage. The alcaligenes faecalis GBW-HB1905 has the capability of efficiently degrading COD in the aquaculture wastewater, and the removal efficiency of the COD in an aquaculture wastewater treatment system can be remarkably promoted and improved.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and relates to a strain of Alcaligenes faecalis GBW-HB1905 and its application. Background technique [0002] At present, the manure discharge produced by farms has caused serious pollution to surface water, groundwater, soil and ambient air, directly affecting people's health, and untreated manure contains a large amount of pollutants. If this organic waste water If it is directly discharged or washed into rivers, lakes and other water bodies with rainwater, it can consume a large amount of dissolved oxygen in the water body, make the water body black and smelly, and cause water pollution. A large amount of sewage discharge will not only seriously affect and destroy the health of the ecological environment, but also cause eutrophication of a large number of rivers, lakes and coastal waters, resulting in a large number of algal blooms, which will damage the health and ecological balance of...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C02F103/20C12R1/05
CPCC02F3/34C02F2103/20C02F2209/08C12N1/20
Inventor 袁绍辉王熙涛张宗国赵金超张晓春孙志刚
Owner QINGDAO SHANGDE BIOTECH
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