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Bioactive material capable of efficiently combining and stimulating bFGF activity function and application

A biologically active and functional technology, applied in the field of biomedical materials, to achieve the effect of protecting against hydrolysis by biological enzymes, high efficiency, and promoting biological activity

Pending Publication Date: 2021-03-23
SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, research and development of drug-loaded materials that bind efficiently and do not affect the biological activity of basic fibroblast growth factor is still a major challenge

Method used

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  • Bioactive material capable of efficiently combining and stimulating bFGF activity function and application
  • Bioactive material capable of efficiently combining and stimulating bFGF activity function and application
  • Bioactive material capable of efficiently combining and stimulating bFGF activity function and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Heparan sulfate proteoglycan efficiently binds and releases basic skin growth factor

[0068] 1. Heparan sulfate mucopolysaccharide efficiently binds alkaline skin growth factor

[0069] First, the combination of heparan sulfate proteoglycan HSPG (0.1 μM) and basic skin growth factor molecules in vitro was detected by enzyme-linked immunosorbent immunoassay (ELISA), and heparin molecules and heparan sulfate molecules of the same concentration were used as control materials. About 100 μl of HSPG (0.1 μM) was added to a 96-well microtiter plate (Thermo Fisher, USA) and incubated overnight at 4°C. Equal concentrations of heparin and heparan sulfate (0.1 [mu]M) were added to separate wells for comparison. After plating with HSPG, plates were dried by discarding excess solution, then washed 2-3 times with phosphate-buffered saline (PBS), and blocked with 2.5% bovine serum albumin containing 0.1% Tween 20 for at least 1 hour at 37°C. After blocking and washing (2-3 times), ...

Embodiment 2

[0075] Using HSPG as biological material for cell culture and detection of bFGF biological activity

[0076] 1. Using HSPG as a biomaterial for the culture of cardiomyocytes

[0077] Hearts were isolated from neonatal SD rats within 3 days of age and digested using a mixed collagenase method. Transfer to a cell culture dish for culture, and conduct the following cell adhesion experiments after stable passage for 3 times. In order to study the effect of HSPG on the adhesion ability of cardiomyocytes, 100 μl of HSPG (0.1 μM) was added to a 96-well plate. As a control, 100 μl of PBS was also added to separate wells. Cardiomyocytes were seeded into each well (5×10 4 / well) and incubated at 37°C for 2 hours. Then, the medium was discarded, the cells were washed two to three times with PBS, and fixed with 4% paraformaldehyde. Cells were stained with Hoechst 33342 (Sigma), and fields were randomly selected through six passes using a Nikon (Japan) confocal microscope and Hoechst-...

Embodiment 3

[0085] The delivery of bFGF using HSPG as a biomaterial can promote the recovery of cardiac function after myocardial infarction

[0086] 1. Using HSPG as the delivery carrier of bFGF to inhibit the hyperplasia of scar after myocardial infarction

[0087] Myocardial infarction was induced by ligating the left anterior descending artery of SD rats. After 4 weeks of treatment with HSPG combined with bFGF in the myocardium, the rats were anesthetized by intraperitoneal injection of 4% pentobarbital sodium. Hearts were fixed in 4% paraformaldehyde to prepare paraffin sections of cardiac tissue. Masson's trichrome staining and hematoxylin and eosin staining were used, and the results were used to analyze scar size and infarct wall thickness. The obtained paraffin sections of cardiac tissue were observed and photographed under a microscope, and the obtained pictures were analyzed using Image J pro software. Such as Figure 3A-Figure 3D As shown, histological analysis of the effec...

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Abstract

The invention discloses a bioactive material capable of efficiently combining and stimulating bFGF activity function and application. The invention also discloses application of the bioactive materialin preparation of a product for treating myocardial infarction. The bioactive material comprises an extracellular matrix molecular material, and the extracellular matrix molecular material comprisesheparan sulfate proteoglycan. According to the bioactive material, binding of heparan sulfate proteoglycan to basic fibroblast growth factor has high efficiency, good stability, and can greatly protect the basic fibroblast growth factor from being hydrolyzed by biological enzymes, and further promote biological activity of the basic fibroblast growth factor, etc. In addition, the bioactive material is used as a carrier of basic fibroblast growth factors, can well help reduce scar accumulation after myocardial infarction, relieve ventricular hypertrophy caused by injury and provide help for cardiac function recovery, and also provides a new thought for drug loading mode change of clinical myocardial infarction treatment.

Description

technical field [0001] The present invention relates to a bioactive material, in particular to an extracellular matrix molecule heparan sulfate proteoglycan (HSPG) material that efficiently binds and can stimulate the activity of basic fibroblast growth factor (bFGF), and it is used as a carrier in The application in promoting cardiac function recovery after myocardial infarction belongs to the technical field of biomedical materials. Background technique [0002] Myocardial infarction (MI), usually caused by coronary occlusion and ischemia, can cause apoptosis of billions of cardiomyocytes and necrosis of left ventricular (LV) myocardium, in addition to concomitant inflammation and scarring. At present, the clinical methods for treating MI mainly include percutaneous transluminal angioplasty (PTCA) and coronary artery bypass grafting (CABG). Mechanical vascular reconstruction can be performed effectively through surgical intervention. However, mechanical vascular reconstruc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/42A61K38/18A61P9/10
CPCA61K47/42A61K38/1825A61P9/10
Inventor 戴建武施家佳赵燕南
Owner SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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